Serine Deficiency Exacerbates Inflammation and Oxidative Stress via Microbiota-Gut-Brain Axis in D-Galactose-Induced Aging Mice
Inflammation and oxidative stress play key roles in the process of aging and age-related diseases. Since serine availability plays important roles in the support of antioxidant and anti-inflammatory defense system, we explored whether serine deficiency affects inflammatory and oxidative status in D-...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2020/5821428 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832568501190000640 |
|---|---|
| author | Fengen Wang Hongbin Zhou Ligang Deng Lei Wang Jingqing Chen Xihong Zhou |
| author_facet | Fengen Wang Hongbin Zhou Ligang Deng Lei Wang Jingqing Chen Xihong Zhou |
| author_sort | Fengen Wang |
| collection | DOAJ |
| description | Inflammation and oxidative stress play key roles in the process of aging and age-related diseases. Since serine availability plays important roles in the support of antioxidant and anti-inflammatory defense system, we explored whether serine deficiency affects inflammatory and oxidative status in D-galactose-induced aging mice. Male mice were randomly assigned into four groups: mice fed a basal diet, mice fed a serine- and glycine-deficient (SGD) diet, mice injected with D-galactose and fed a basal diet, and mice injected with D-galactose and fed an SGD diet. The results showed that D-galactose resulted in oxidative and inflammatory responses, while serine deficiency alone showed no such effects. However, serine deficiency significantly exacerbated oxidative stress and inflammation in D-galactose-treated mice. The composition of fecal microbiota was affected by D-galactose injection, which was characterized by decreased microbiota diversity and downregulated ratio of Firmicutes/Bacteroidetes, as well as decreased proportion of Clostridium XIVa. Furthermore, serine deficiency exacerbated these changes. Additionally, serine deficiency in combination with D-galactose injection significantly decreased fecal butyric acid content and gene expression of short-chain fatty acid transporters (Slc16a3 and Slc16a7) and receptor (Gpr109a) in the brain. Finally, serine deficiency exacerbated the decrease of expression of phosphorylated AMPK and the increase of expression of phosphorylated NFκB p65, which were caused by D-galactose injection. In conclusion, our results suggested that serine deficiency exacerbated inflammation and oxidative stress in D-galactose-induced aging mice. The involved mechanisms might be partially attributed to the changes in the microbiota-gut-brain axis affected by serine deficiency. |
| format | Article |
| id | doaj-art-6923dbaa0c5244c0833968b1c3bcbf37 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-6923dbaa0c5244c0833968b1c3bcbf372025-02-03T00:58:57ZengWileyMediators of Inflammation0962-93511466-18612020-01-01202010.1155/2020/58214285821428Serine Deficiency Exacerbates Inflammation and Oxidative Stress via Microbiota-Gut-Brain Axis in D-Galactose-Induced Aging MiceFengen Wang0Hongbin Zhou1Ligang Deng2Lei Wang3Jingqing Chen4Xihong Zhou5Institute of Quality Standard & Testing Technology for Agro-Products, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaDalian Chengsan Husbandry Co., Ltd., Dalian, ChinaInstitute of Quality Standard & Testing Technology for Agro-Products, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaInstitute of Quality Standard & Testing Technology for Agro-Products, Shandong Academy of Agricultural Sciences, Jinan 250100, ChinaState Key Laboratory of Animal Nutrition, China Agricultural University, Beijing 100193, ChinaKey Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, ChinaInflammation and oxidative stress play key roles in the process of aging and age-related diseases. Since serine availability plays important roles in the support of antioxidant and anti-inflammatory defense system, we explored whether serine deficiency affects inflammatory and oxidative status in D-galactose-induced aging mice. Male mice were randomly assigned into four groups: mice fed a basal diet, mice fed a serine- and glycine-deficient (SGD) diet, mice injected with D-galactose and fed a basal diet, and mice injected with D-galactose and fed an SGD diet. The results showed that D-galactose resulted in oxidative and inflammatory responses, while serine deficiency alone showed no such effects. However, serine deficiency significantly exacerbated oxidative stress and inflammation in D-galactose-treated mice. The composition of fecal microbiota was affected by D-galactose injection, which was characterized by decreased microbiota diversity and downregulated ratio of Firmicutes/Bacteroidetes, as well as decreased proportion of Clostridium XIVa. Furthermore, serine deficiency exacerbated these changes. Additionally, serine deficiency in combination with D-galactose injection significantly decreased fecal butyric acid content and gene expression of short-chain fatty acid transporters (Slc16a3 and Slc16a7) and receptor (Gpr109a) in the brain. Finally, serine deficiency exacerbated the decrease of expression of phosphorylated AMPK and the increase of expression of phosphorylated NFκB p65, which were caused by D-galactose injection. In conclusion, our results suggested that serine deficiency exacerbated inflammation and oxidative stress in D-galactose-induced aging mice. The involved mechanisms might be partially attributed to the changes in the microbiota-gut-brain axis affected by serine deficiency.http://dx.doi.org/10.1155/2020/5821428 |
| spellingShingle | Fengen Wang Hongbin Zhou Ligang Deng Lei Wang Jingqing Chen Xihong Zhou Serine Deficiency Exacerbates Inflammation and Oxidative Stress via Microbiota-Gut-Brain Axis in D-Galactose-Induced Aging Mice Mediators of Inflammation |
| title | Serine Deficiency Exacerbates Inflammation and Oxidative Stress via Microbiota-Gut-Brain Axis in D-Galactose-Induced Aging Mice |
| title_full | Serine Deficiency Exacerbates Inflammation and Oxidative Stress via Microbiota-Gut-Brain Axis in D-Galactose-Induced Aging Mice |
| title_fullStr | Serine Deficiency Exacerbates Inflammation and Oxidative Stress via Microbiota-Gut-Brain Axis in D-Galactose-Induced Aging Mice |
| title_full_unstemmed | Serine Deficiency Exacerbates Inflammation and Oxidative Stress via Microbiota-Gut-Brain Axis in D-Galactose-Induced Aging Mice |
| title_short | Serine Deficiency Exacerbates Inflammation and Oxidative Stress via Microbiota-Gut-Brain Axis in D-Galactose-Induced Aging Mice |
| title_sort | serine deficiency exacerbates inflammation and oxidative stress via microbiota gut brain axis in d galactose induced aging mice |
| url | http://dx.doi.org/10.1155/2020/5821428 |
| work_keys_str_mv | AT fengenwang serinedeficiencyexacerbatesinflammationandoxidativestressviamicrobiotagutbrainaxisindgalactoseinducedagingmice AT hongbinzhou serinedeficiencyexacerbatesinflammationandoxidativestressviamicrobiotagutbrainaxisindgalactoseinducedagingmice AT ligangdeng serinedeficiencyexacerbatesinflammationandoxidativestressviamicrobiotagutbrainaxisindgalactoseinducedagingmice AT leiwang serinedeficiencyexacerbatesinflammationandoxidativestressviamicrobiotagutbrainaxisindgalactoseinducedagingmice AT jingqingchen serinedeficiencyexacerbatesinflammationandoxidativestressviamicrobiotagutbrainaxisindgalactoseinducedagingmice AT xihongzhou serinedeficiencyexacerbatesinflammationandoxidativestressviamicrobiotagutbrainaxisindgalactoseinducedagingmice |