Urinary apolipoprotein A4 as a biomarker for renal allograft injury in kidney transplant recipients.
Chronic renal allograft injury (CRAI) is a major cause of allograft loss in kidney transplant recipients (KTRs). The aim of this study was to evaluate the associations of urinary apolipoprotein A4 (ApoA-IV) levels with renal function and rapid renal function decline in KTRs. This study included 50 K...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2025-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0324529 |
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| author | Youn Kyung Kee Dong Ho Shin Jieun Oh Ji Young Park Dong Hyun Kim Kyungjai Ko Samuel Lee Hee Jung Jeon |
| author_facet | Youn Kyung Kee Dong Ho Shin Jieun Oh Ji Young Park Dong Hyun Kim Kyungjai Ko Samuel Lee Hee Jung Jeon |
| author_sort | Youn Kyung Kee |
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| description | Chronic renal allograft injury (CRAI) is a major cause of allograft loss in kidney transplant recipients (KTRs). The aim of this study was to evaluate the associations of urinary apolipoprotein A4 (ApoA-IV) levels with renal function and rapid renal function decline in KTRs. This study included 50 KTRs. Proteomic analysis via liquid chromatography‒mass spectrometry and tandem mass spectrometry (LC-MS/MS) was performed to identify potential urinary biomarkers. The SWATH (sequential window acquisition of all theoretical mass spectra) method was used for protein quantification. Urinary ApoA-IV levels were validated by enzyme-linked immunosorbent assay (ELISA). Rapid renal function decline was defined as an estimated glomerular filtration rate (GFR) decrease of >3 mL/min/1.73 m2 per year or initiation of dialysis. The log-transformed urinary ApoA-IV levels measured by ELISA had a significantly inverse correlation with the estimated GFR (r = -0.72, P < 0.001). Moreover, urinary ApoA-IV levels were higher in patients with rapid renal function decline than in those with stable renal function (215.4 ± 181.8 μg/mL vs. 42.5 ± 72.4 μg/mL, P = 0.001). Univariate logistic regression analysis revealed that log-transformed urinary ApoA-IV levels were significantly associated with rapid renal function decline (odds ratio [OR] 6.70, 95% confidence interval [CI] 2.56-22.83; P < 0.001). Multiple logistic regression showed urinary ApoA-IV levels remained a significant risk factor for rapid renal function decline (OR 4.10, 95% CI 1.10-19.55; P = 0.047). ROC curve analysis revealed the area under the curve (AUC) of 0.834 (95% CI 0.722-0.945, P < 0.001) for urinary ApoA-IV levels in predicting rapid renal function decline. Our results suggest that urinary ApoA-IV levels might be a potential biomarker for renal allograft function and could be used as a predictor for rapid renal function decline in KTRs. |
| format | Article |
| id | doaj-art-690fa9844e9d4cafb21cc72fa70ca916 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj-art-690fa9844e9d4cafb21cc72fa70ca9162025-08-20T03:53:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01205e032452910.1371/journal.pone.0324529Urinary apolipoprotein A4 as a biomarker for renal allograft injury in kidney transplant recipients.Youn Kyung KeeDong Ho ShinJieun OhJi Young ParkDong Hyun KimKyungjai KoSamuel LeeHee Jung JeonChronic renal allograft injury (CRAI) is a major cause of allograft loss in kidney transplant recipients (KTRs). The aim of this study was to evaluate the associations of urinary apolipoprotein A4 (ApoA-IV) levels with renal function and rapid renal function decline in KTRs. This study included 50 KTRs. Proteomic analysis via liquid chromatography‒mass spectrometry and tandem mass spectrometry (LC-MS/MS) was performed to identify potential urinary biomarkers. The SWATH (sequential window acquisition of all theoretical mass spectra) method was used for protein quantification. Urinary ApoA-IV levels were validated by enzyme-linked immunosorbent assay (ELISA). Rapid renal function decline was defined as an estimated glomerular filtration rate (GFR) decrease of >3 mL/min/1.73 m2 per year or initiation of dialysis. The log-transformed urinary ApoA-IV levels measured by ELISA had a significantly inverse correlation with the estimated GFR (r = -0.72, P < 0.001). Moreover, urinary ApoA-IV levels were higher in patients with rapid renal function decline than in those with stable renal function (215.4 ± 181.8 μg/mL vs. 42.5 ± 72.4 μg/mL, P = 0.001). Univariate logistic regression analysis revealed that log-transformed urinary ApoA-IV levels were significantly associated with rapid renal function decline (odds ratio [OR] 6.70, 95% confidence interval [CI] 2.56-22.83; P < 0.001). Multiple logistic regression showed urinary ApoA-IV levels remained a significant risk factor for rapid renal function decline (OR 4.10, 95% CI 1.10-19.55; P = 0.047). ROC curve analysis revealed the area under the curve (AUC) of 0.834 (95% CI 0.722-0.945, P < 0.001) for urinary ApoA-IV levels in predicting rapid renal function decline. Our results suggest that urinary ApoA-IV levels might be a potential biomarker for renal allograft function and could be used as a predictor for rapid renal function decline in KTRs.https://doi.org/10.1371/journal.pone.0324529 |
| spellingShingle | Youn Kyung Kee Dong Ho Shin Jieun Oh Ji Young Park Dong Hyun Kim Kyungjai Ko Samuel Lee Hee Jung Jeon Urinary apolipoprotein A4 as a biomarker for renal allograft injury in kidney transplant recipients. PLoS ONE |
| title | Urinary apolipoprotein A4 as a biomarker for renal allograft injury in kidney transplant recipients. |
| title_full | Urinary apolipoprotein A4 as a biomarker for renal allograft injury in kidney transplant recipients. |
| title_fullStr | Urinary apolipoprotein A4 as a biomarker for renal allograft injury in kidney transplant recipients. |
| title_full_unstemmed | Urinary apolipoprotein A4 as a biomarker for renal allograft injury in kidney transplant recipients. |
| title_short | Urinary apolipoprotein A4 as a biomarker for renal allograft injury in kidney transplant recipients. |
| title_sort | urinary apolipoprotein a4 as a biomarker for renal allograft injury in kidney transplant recipients |
| url | https://doi.org/10.1371/journal.pone.0324529 |
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