Cellular reactions to three-dimensional matrices of polylactic acid and hydroxyapatite generated by 3D-printing
The purpose of this study is to estimate Ex vivo physicochemical and biological features of three-dimensional (3D) biodegradable matrices “polylactic acid/calcium phosphates” (hereafter 3D composites) designed with the help of additive technologies (3D printing) as potential materials for bone tissu...
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Siberian State Medical University (Tomsk)
2017-01-01
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| Series: | Бюллетень сибирской медицины |
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| Online Access: | https://bulletin.ssmu.ru/jour/article/view/729 |
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| author | T. V. Druzhinina S. Ya. Talalaev N. P. Zakirov S. V. Shchadenko Sh. A. Khabibulin I. A. Khlusov L. S. Litvinova |
| author_facet | T. V. Druzhinina S. Ya. Talalaev N. P. Zakirov S. V. Shchadenko Sh. A. Khabibulin I. A. Khlusov L. S. Litvinova |
| author_sort | T. V. Druzhinina |
| collection | DOAJ |
| description | The purpose of this study is to estimate Ex vivo physicochemical and biological features of three-dimensional (3D) biodegradable matrices “polylactic acid/calcium phosphates” (hereafter 3D composites) designed with the help of additive technologies (3D printing) as potential materials for bone tissue regeneration.Materials and methods. Experimental samples (disks 1,2–1,6 mm thick, and 11 mm or 8 mm in diameter) of composite biodegradable 3D matrices (hereafter 3D composites) have been produced from initial mixture of 95 mas% polylactic acid (PLA) and 5 mas% hydroxyapatite (HAP). Computer-aided design method, Blender software and fused filament fabrication (FFF; fiber diameter 1,75 mm) with 3D printing were used in sample production. 100 mas% PLA disks served as control. One of the sample surfaces was textured with 0,3–0,5 mm wide grooves. Physicochemical properties of 11 mm disks (geometry, mass, morphology, roughness, electrostatic voltage, surface wettability, and element composition) were studied. Biological trials included the evaluation of 24-hour cytotoxicity of 8 mm samples in culture of mononuclear leukocytes of a healthy volunteer or human Jurkat T cell leukemia-derived cell line (hereafter Jurkat T cells). Moreover, osteogenic potential of 11 mm disks was determined in 21-day culture of human adipose-derived multipotent mesenchymal stem cells (AMMSCs) be means of osteocalcin secretion and intercellular matrix mineralization visualized by alizarin red S staining.Results. The features of PLA-HAP 3D composites generated by 3D printing correspond to physicochemical parameters which are crucial for bone tissue recovery. In case of small amount of calcium and phosphorus they facilitated ex vivo mineralization of extracellular matrix formed in AMMSCs culture. The number of died (by necrosis, mainly) leukemic Jurkat T cells but not mononuclear leukocytes of a health volunteer increased to 9–10% in 24-hour in vitro contact with PLA-HAP 3D composites unlike PLA samples alone.Conclusion. Polar reaction of tumor and normal cells to PLA-HAP samples in case of increasing amount of calcium phosphates in 3D-composite may be valuable for the development of new materials used for osteosynthesis of fractures and endoprosthesis in patients with hematological malignancies. |
| format | Article |
| id | doaj-art-68f37e1baa5e45cdb000dbd35ee6b89f |
| institution | Kabale University |
| issn | 1682-0363 1819-3684 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Siberian State Medical University (Tomsk) |
| record_format | Article |
| series | Бюллетень сибирской медицины |
| spelling | doaj-art-68f37e1baa5e45cdb000dbd35ee6b89f2025-08-20T03:38:08ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842017-01-01155162910.20538/1682-0363-2016-5-16-29598Cellular reactions to three-dimensional matrices of polylactic acid and hydroxyapatite generated by 3D-printingT. V. Druzhinina0S. Ya. Talalaev1N. P. Zakirov2S. V. Shchadenko3Sh. A. Khabibulin4I. A. Khlusov5L. S. Litvinova6Federal State Unitary Enterprise “Experimental production workshops”, Federal Medical and Biological AgencyFederal State Unitary Enterprise “Experimental production workshops”, Federal Medical and Biological AgencyFederal State Unitary Enterprise “Experimental production workshops”, Federal Medical and Biological AgencySiberian State Medical University; National Research Tomsk Polytechnic UniversityNational Research Tomsk Polytechnic UniversitySiberian State Medical University; National Research Tomsk Polytechnic University; Bioconstructor-S Ltd.Immanuel Kant Baltic Federal UniversityThe purpose of this study is to estimate Ex vivo physicochemical and biological features of three-dimensional (3D) biodegradable matrices “polylactic acid/calcium phosphates” (hereafter 3D composites) designed with the help of additive technologies (3D printing) as potential materials for bone tissue regeneration.Materials and methods. Experimental samples (disks 1,2–1,6 mm thick, and 11 mm or 8 mm in diameter) of composite biodegradable 3D matrices (hereafter 3D composites) have been produced from initial mixture of 95 mas% polylactic acid (PLA) and 5 mas% hydroxyapatite (HAP). Computer-aided design method, Blender software and fused filament fabrication (FFF; fiber diameter 1,75 mm) with 3D printing were used in sample production. 100 mas% PLA disks served as control. One of the sample surfaces was textured with 0,3–0,5 mm wide grooves. Physicochemical properties of 11 mm disks (geometry, mass, morphology, roughness, electrostatic voltage, surface wettability, and element composition) were studied. Biological trials included the evaluation of 24-hour cytotoxicity of 8 mm samples in culture of mononuclear leukocytes of a healthy volunteer or human Jurkat T cell leukemia-derived cell line (hereafter Jurkat T cells). Moreover, osteogenic potential of 11 mm disks was determined in 21-day culture of human adipose-derived multipotent mesenchymal stem cells (AMMSCs) be means of osteocalcin secretion and intercellular matrix mineralization visualized by alizarin red S staining.Results. The features of PLA-HAP 3D composites generated by 3D printing correspond to physicochemical parameters which are crucial for bone tissue recovery. In case of small amount of calcium and phosphorus they facilitated ex vivo mineralization of extracellular matrix formed in AMMSCs culture. The number of died (by necrosis, mainly) leukemic Jurkat T cells but not mononuclear leukocytes of a health volunteer increased to 9–10% in 24-hour in vitro contact with PLA-HAP 3D composites unlike PLA samples alone.Conclusion. Polar reaction of tumor and normal cells to PLA-HAP samples in case of increasing amount of calcium phosphates in 3D-composite may be valuable for the development of new materials used for osteosynthesis of fractures and endoprosthesis in patients with hematological malignancies.https://bulletin.ssmu.ru/jour/article/view/7293d-simulationprototypingbiodegradable disksin vitrocytotoxicitystromal stem cellsosteocalcin secretionalizarin red staining |
| spellingShingle | T. V. Druzhinina S. Ya. Talalaev N. P. Zakirov S. V. Shchadenko Sh. A. Khabibulin I. A. Khlusov L. S. Litvinova Cellular reactions to three-dimensional matrices of polylactic acid and hydroxyapatite generated by 3D-printing Бюллетень сибирской медицины 3d-simulation prototyping biodegradable disks in vitro cytotoxicity stromal stem cells osteocalcin secretion alizarin red staining |
| title | Cellular reactions to three-dimensional matrices of polylactic acid and hydroxyapatite generated by 3D-printing |
| title_full | Cellular reactions to three-dimensional matrices of polylactic acid and hydroxyapatite generated by 3D-printing |
| title_fullStr | Cellular reactions to three-dimensional matrices of polylactic acid and hydroxyapatite generated by 3D-printing |
| title_full_unstemmed | Cellular reactions to three-dimensional matrices of polylactic acid and hydroxyapatite generated by 3D-printing |
| title_short | Cellular reactions to three-dimensional matrices of polylactic acid and hydroxyapatite generated by 3D-printing |
| title_sort | cellular reactions to three dimensional matrices of polylactic acid and hydroxyapatite generated by 3d printing |
| topic | 3d-simulation prototyping biodegradable disks in vitro cytotoxicity stromal stem cells osteocalcin secretion alizarin red staining |
| url | https://bulletin.ssmu.ru/jour/article/view/729 |
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