Effects of resource packaging on the adaptative and pleiotropic consequences of evolution

Abstract Adaptation to an environment is enabled by the accumulation of beneficial mutations. How do adaptive trajectories and pleiotropic effects of adaptation change in response to “subtle” changes in the environment? Since there exists no molecular framework to quantify “subtle” environmental cha...

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Main Authors: Neetika Ahlawat, Pavithra Venkataraman, Raman Gulab Brajesh, Supreet Saini
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:npj Systems Biology and Applications
Online Access:https://doi.org/10.1038/s41540-025-00558-2
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author Neetika Ahlawat
Pavithra Venkataraman
Raman Gulab Brajesh
Supreet Saini
author_facet Neetika Ahlawat
Pavithra Venkataraman
Raman Gulab Brajesh
Supreet Saini
author_sort Neetika Ahlawat
collection DOAJ
description Abstract Adaptation to an environment is enabled by the accumulation of beneficial mutations. How do adaptive trajectories and pleiotropic effects of adaptation change in response to “subtle” changes in the environment? Since there exists no molecular framework to quantify “subtle” environmental change, designing experiments to answer this question has been challenging. In this work, we address this question by studying the effects of evolution in environments which differ solely in the way sugars are presented to a bacterial population. Specifically, we focus on glucose and galactose, which can be supplied to an E. coli population as a mixture of glucose and galactose, lactose, or melibiose. We evolve six replicate populations of E coli for 300 generations in these three chemically correlated or “synonymous” environments, and show that the adaptive responses of these populations are not similar. When tested for pleiotropic effects of fitness in a range of non-synonymous environments, our results show that despite uncorrelated adaptive changes, the nature of pleiotropic effects is largely predictable based on the fitness of the ancestor in the non-home environments. Overall, our results highlight how subtle changes in the environment can alter adaptation, but despite sequence-level variations, pleiotropy is qualitatively predictable.
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spelling doaj-art-68e90e12eac44585bff7ad893ac580da2025-08-20T03:42:55ZengNature Portfolionpj Systems Biology and Applications2056-71892025-07-011111910.1038/s41540-025-00558-2Effects of resource packaging on the adaptative and pleiotropic consequences of evolutionNeetika Ahlawat0Pavithra Venkataraman1Raman Gulab Brajesh2Supreet Saini3Department of Chemical Engineering, Indian Institute of Technology BombayDepartment of Chemical Engineering, Indian Institute of Technology BombayDepartment of Chemical Engineering, Indian Institute of Technology BombayDepartment of Chemical Engineering, Indian Institute of Technology BombayAbstract Adaptation to an environment is enabled by the accumulation of beneficial mutations. How do adaptive trajectories and pleiotropic effects of adaptation change in response to “subtle” changes in the environment? Since there exists no molecular framework to quantify “subtle” environmental change, designing experiments to answer this question has been challenging. In this work, we address this question by studying the effects of evolution in environments which differ solely in the way sugars are presented to a bacterial population. Specifically, we focus on glucose and galactose, which can be supplied to an E. coli population as a mixture of glucose and galactose, lactose, or melibiose. We evolve six replicate populations of E coli for 300 generations in these three chemically correlated or “synonymous” environments, and show that the adaptive responses of these populations are not similar. When tested for pleiotropic effects of fitness in a range of non-synonymous environments, our results show that despite uncorrelated adaptive changes, the nature of pleiotropic effects is largely predictable based on the fitness of the ancestor in the non-home environments. Overall, our results highlight how subtle changes in the environment can alter adaptation, but despite sequence-level variations, pleiotropy is qualitatively predictable.https://doi.org/10.1038/s41540-025-00558-2
spellingShingle Neetika Ahlawat
Pavithra Venkataraman
Raman Gulab Brajesh
Supreet Saini
Effects of resource packaging on the adaptative and pleiotropic consequences of evolution
npj Systems Biology and Applications
title Effects of resource packaging on the adaptative and pleiotropic consequences of evolution
title_full Effects of resource packaging on the adaptative and pleiotropic consequences of evolution
title_fullStr Effects of resource packaging on the adaptative and pleiotropic consequences of evolution
title_full_unstemmed Effects of resource packaging on the adaptative and pleiotropic consequences of evolution
title_short Effects of resource packaging on the adaptative and pleiotropic consequences of evolution
title_sort effects of resource packaging on the adaptative and pleiotropic consequences of evolution
url https://doi.org/10.1038/s41540-025-00558-2
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