Comprehensive review on anti-obesity effects of plant-derived compounds: Evidence from 3T3-L1 adipocytes and high-fat diet models
Obesity, a multifactorial chronic disease, poses a growing global health concern, contributing to increased incidences of type 2 diabetes, cardiovascular diseases, osteoarthritis, and several cancers. Despite various pharmacological attempts targeting lipid metabolism enzymes, the associated adverse...
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Elsevier
2025-06-01
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| Series: | Aspects of Molecular Medicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2949688825000279 |
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| author | Sachin Gudasi Mrityunjaya B. Patil |
| author_facet | Sachin Gudasi Mrityunjaya B. Patil |
| author_sort | Sachin Gudasi |
| collection | DOAJ |
| description | Obesity, a multifactorial chronic disease, poses a growing global health concern, contributing to increased incidences of type 2 diabetes, cardiovascular diseases, osteoarthritis, and several cancers. Despite various pharmacological attempts targeting lipid metabolism enzymes, the associated adverse effects have led to numerous drug withdrawals, underscoring the urgent need for safer and more effective therapeutic strategies. In this context, the present study explores the novel therapeutic potential of plant-derived bioactives, specifically formulated using gold nanoparticles (GNPs), for the management of obesity. We systematically investigated the modulation of critical adipogenic and lipogenic regulatory proteins—C/EBP-α, PPAR-α, perilipin-1, adiponectin, FABP4, FAS, and ACC in 3T3-L1 pre-adipocytes and high-fat diet-induced obese mice. Our findings demonstrate that GNP-encapsulated phytoconstituents significantly reduce intracellular lipid accumulation by activating AMPK, a key energy sensor that downregulates pro-adipogenic and lipogenic genes (PPAR-α, C/EBP-α, AP2, SREBP-1c, ACC1, FAS, and LPL), while concurrently upregulating lipolytic and thermogenic genes (HSL, PGC-1α, and SIRT1) and enhancing adiponectin expression. The novelty of this study lies in the synergistic application of nanotechnology and traditional plant-based therapeutics to target obesity at a molecular level, offering a dual advantage of enhanced bioavailability and targeted action. These outcomes provide compelling evidence for the use of functionalized nanoparticles as a next-generation anti-obesity strategy, with potential translational value for clinical application. |
| format | Article |
| id | doaj-art-68e55c8394f44225b0b5ba0282f755c8 |
| institution | DOAJ |
| issn | 2949-6888 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aspects of Molecular Medicine |
| spelling | doaj-art-68e55c8394f44225b0b5ba0282f755c82025-08-20T03:07:57ZengElsevierAspects of Molecular Medicine2949-68882025-06-01510008910.1016/j.amolm.2025.100089Comprehensive review on anti-obesity effects of plant-derived compounds: Evidence from 3T3-L1 adipocytes and high-fat diet modelsSachin Gudasi0Mrityunjaya B. Patil1Department of Pharmacognosy, KLE College of Pharmacy, KLE Academy of Higher Education and Research, Nehru Nagar, Belagavi, Karnataka, 590010, IndiaCorresponding author.; Department of Pharmacognosy, KLE College of Pharmacy, KLE Academy of Higher Education and Research, Nehru Nagar, Belagavi, Karnataka, 590010, IndiaObesity, a multifactorial chronic disease, poses a growing global health concern, contributing to increased incidences of type 2 diabetes, cardiovascular diseases, osteoarthritis, and several cancers. Despite various pharmacological attempts targeting lipid metabolism enzymes, the associated adverse effects have led to numerous drug withdrawals, underscoring the urgent need for safer and more effective therapeutic strategies. In this context, the present study explores the novel therapeutic potential of plant-derived bioactives, specifically formulated using gold nanoparticles (GNPs), for the management of obesity. We systematically investigated the modulation of critical adipogenic and lipogenic regulatory proteins—C/EBP-α, PPAR-α, perilipin-1, adiponectin, FABP4, FAS, and ACC in 3T3-L1 pre-adipocytes and high-fat diet-induced obese mice. Our findings demonstrate that GNP-encapsulated phytoconstituents significantly reduce intracellular lipid accumulation by activating AMPK, a key energy sensor that downregulates pro-adipogenic and lipogenic genes (PPAR-α, C/EBP-α, AP2, SREBP-1c, ACC1, FAS, and LPL), while concurrently upregulating lipolytic and thermogenic genes (HSL, PGC-1α, and SIRT1) and enhancing adiponectin expression. The novelty of this study lies in the synergistic application of nanotechnology and traditional plant-based therapeutics to target obesity at a molecular level, offering a dual advantage of enhanced bioavailability and targeted action. These outcomes provide compelling evidence for the use of functionalized nanoparticles as a next-generation anti-obesity strategy, with potential translational value for clinical application.http://www.sciencedirect.com/science/article/pii/S29496888250002793T3-L1 adipocytesHigh fat dietAdipogenicLipogenicLipolytic |
| spellingShingle | Sachin Gudasi Mrityunjaya B. Patil Comprehensive review on anti-obesity effects of plant-derived compounds: Evidence from 3T3-L1 adipocytes and high-fat diet models Aspects of Molecular Medicine 3T3-L1 adipocytes High fat diet Adipogenic Lipogenic Lipolytic |
| title | Comprehensive review on anti-obesity effects of plant-derived compounds: Evidence from 3T3-L1 adipocytes and high-fat diet models |
| title_full | Comprehensive review on anti-obesity effects of plant-derived compounds: Evidence from 3T3-L1 adipocytes and high-fat diet models |
| title_fullStr | Comprehensive review on anti-obesity effects of plant-derived compounds: Evidence from 3T3-L1 adipocytes and high-fat diet models |
| title_full_unstemmed | Comprehensive review on anti-obesity effects of plant-derived compounds: Evidence from 3T3-L1 adipocytes and high-fat diet models |
| title_short | Comprehensive review on anti-obesity effects of plant-derived compounds: Evidence from 3T3-L1 adipocytes and high-fat diet models |
| title_sort | comprehensive review on anti obesity effects of plant derived compounds evidence from 3t3 l1 adipocytes and high fat diet models |
| topic | 3T3-L1 adipocytes High fat diet Adipogenic Lipogenic Lipolytic |
| url | http://www.sciencedirect.com/science/article/pii/S2949688825000279 |
| work_keys_str_mv | AT sachingudasi comprehensivereviewonantiobesityeffectsofplantderivedcompoundsevidencefrom3t3l1adipocytesandhighfatdietmodels AT mrityunjayabpatil comprehensivereviewonantiobesityeffectsofplantderivedcompoundsevidencefrom3t3l1adipocytesandhighfatdietmodels |