Micro‐CT Analysis of Rodent Temporal Bones: Identifying Optimal Species for Otological Research
ABSTRACT Objectives Rodents are used in most otological research studies and the choice of the most appropriate animal model may be crucial in studies of ear diseases and in the development of effective treatments. Here, we used micro‐CT to compare temporal bone anatomy between four rodent model ani...
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| Format: | Article |
| Language: | English |
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Wiley
2025-06-01
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| Series: | Laryngoscope Investigative Otolaryngology |
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| Online Access: | https://doi.org/10.1002/lio2.70190 |
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| author | Hannah Daoudi Evelyne Ferrary Cerine Moula Florence Agnely Amélie Bochot Catherine Cailleau Yann Nguyen Ghizlène Lahlou Renato Torres |
| author_facet | Hannah Daoudi Evelyne Ferrary Cerine Moula Florence Agnely Amélie Bochot Catherine Cailleau Yann Nguyen Ghizlène Lahlou Renato Torres |
| author_sort | Hannah Daoudi |
| collection | DOAJ |
| description | ABSTRACT Objectives Rodents are used in most otological research studies and the choice of the most appropriate animal model may be crucial in studies of ear diseases and in the development of effective treatments. Here, we used micro‐CT to compare temporal bone anatomy between four rodent model animals (guinea pigs, gerbils, rats, and mice) and humans, aiming to better characterize the anatomy of the inner and middle ear, and facial nerve to support informed animal model selection in otologic research. Methods We generated three‐dimensional reconstructions and measured the various middle (tympanic membrane, ossicular chain, and facial nerve) and inner (cochlea, vestibular labyrinth) ear structures. Results Each structure of the middle or inner ear of each rodent was described and measured. Conclusion This micro‐CT analysis of rodents can guide researchers in their choice of the most suitable middle or inner ear models based on the specific anatomic area of interest. Our findings highlight the strengths and limitations of each species, providing essential insight that could enhance the precision and applicability of otological studies. Level of Evidence 4. |
| format | Article |
| id | doaj-art-68e45e974b094fc686e12035647f4ef2 |
| institution | Kabale University |
| issn | 2378-8038 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Laryngoscope Investigative Otolaryngology |
| spelling | doaj-art-68e45e974b094fc686e12035647f4ef22025-08-20T03:27:07ZengWileyLaryngoscope Investigative Otolaryngology2378-80382025-06-01103n/an/a10.1002/lio2.70190Micro‐CT Analysis of Rodent Temporal Bones: Identifying Optimal Species for Otological ResearchHannah Daoudi0Evelyne Ferrary1Cerine Moula2Florence Agnely3Amélie Bochot4Catherine Cailleau5Yann Nguyen6Ghizlène Lahlou7Renato Torres8Université Paris Cité, Institut Pasteur, AP‐HP, Inserm, Fondation Pour l'audition, Institut de l'audition, IHU Re‐Connect F‐75012, Technologies and Gene Therapy for Deafness Paris FranceUniversité Paris Cité, Institut Pasteur, AP‐HP, Inserm, Fondation Pour l'audition, Institut de l'audition, IHU Re‐Connect F‐75012, Technologies and Gene Therapy for Deafness Paris FranceParis‐Saclay University, CNRS, Galien Paris‐Saclay Institute Orsay FranceParis‐Saclay University, CNRS, Galien Paris‐Saclay Institute Orsay FranceParis‐Saclay University, CNRS, Galien Paris‐Saclay Institute Orsay FranceParis‐Saclay University, CNRS, Galien Paris‐Saclay Institute Orsay FranceUniversité Paris Cité, Institut Pasteur, AP‐HP, Inserm, Fondation Pour l'audition, Institut de l'audition, IHU Re‐Connect F‐75012, Technologies and Gene Therapy for Deafness Paris FranceUniversité Paris Cité, Institut Pasteur, AP‐HP, Inserm, Fondation Pour l'audition, Institut de l'audition, IHU Re‐Connect F‐75012, Technologies and Gene Therapy for Deafness Paris FranceUniversité Paris Cité, Institut Pasteur, AP‐HP, Inserm, Fondation Pour l'audition, Institut de l'audition, IHU Re‐Connect F‐75012, Technologies and Gene Therapy for Deafness Paris FranceABSTRACT Objectives Rodents are used in most otological research studies and the choice of the most appropriate animal model may be crucial in studies of ear diseases and in the development of effective treatments. Here, we used micro‐CT to compare temporal bone anatomy between four rodent model animals (guinea pigs, gerbils, rats, and mice) and humans, aiming to better characterize the anatomy of the inner and middle ear, and facial nerve to support informed animal model selection in otologic research. Methods We generated three‐dimensional reconstructions and measured the various middle (tympanic membrane, ossicular chain, and facial nerve) and inner (cochlea, vestibular labyrinth) ear structures. Results Each structure of the middle or inner ear of each rodent was described and measured. Conclusion This micro‐CT analysis of rodents can guide researchers in their choice of the most suitable middle or inner ear models based on the specific anatomic area of interest. Our findings highlight the strengths and limitations of each species, providing essential insight that could enhance the precision and applicability of otological studies. Level of Evidence 4.https://doi.org/10.1002/lio2.70190animal modelcochleaimagingmiddle ear anatomythree‐dimensional anatomy |
| spellingShingle | Hannah Daoudi Evelyne Ferrary Cerine Moula Florence Agnely Amélie Bochot Catherine Cailleau Yann Nguyen Ghizlène Lahlou Renato Torres Micro‐CT Analysis of Rodent Temporal Bones: Identifying Optimal Species for Otological Research Laryngoscope Investigative Otolaryngology animal model cochlea imaging middle ear anatomy three‐dimensional anatomy |
| title | Micro‐CT Analysis of Rodent Temporal Bones: Identifying Optimal Species for Otological Research |
| title_full | Micro‐CT Analysis of Rodent Temporal Bones: Identifying Optimal Species for Otological Research |
| title_fullStr | Micro‐CT Analysis of Rodent Temporal Bones: Identifying Optimal Species for Otological Research |
| title_full_unstemmed | Micro‐CT Analysis of Rodent Temporal Bones: Identifying Optimal Species for Otological Research |
| title_short | Micro‐CT Analysis of Rodent Temporal Bones: Identifying Optimal Species for Otological Research |
| title_sort | micro ct analysis of rodent temporal bones identifying optimal species for otological research |
| topic | animal model cochlea imaging middle ear anatomy three‐dimensional anatomy |
| url | https://doi.org/10.1002/lio2.70190 |
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