Analysis of fatty acid composition and miRNA profile in adipose tissue after application of Allium mongolicum Regel extract in diet of sheep
Extensive regulation of microRNAs (miRNAs) that belong to a type of endogenous small noncoding RNAs on gene expression in sheep has been recognised. Meanwhile, utilisation of water extract of Allium mongolicum Regel (WEA) in diet of sheep has been proved to regulate fatty acid composition and flavou...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Italian Journal of Animal Science |
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| Online Access: | http://dx.doi.org/10.1080/1828051X.2023.2222743 |
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| author | Zejun Fan Changjin Ao Khas Erdene Chen Bai Ruli Sa Qina Cao Yankai Zheng |
| author_facet | Zejun Fan Changjin Ao Khas Erdene Chen Bai Ruli Sa Qina Cao Yankai Zheng |
| author_sort | Zejun Fan |
| collection | DOAJ |
| description | Extensive regulation of microRNAs (miRNAs) that belong to a type of endogenous small noncoding RNAs on gene expression in sheep has been recognised. Meanwhile, utilisation of water extract of Allium mongolicum Regel (WEA) in diet of sheep has been proved to regulate fatty acid composition and flavour in mutton. However, the regulatory mechanism remains unclear. We conducted miRNA and mRNA sequencing in this study. Briefly, 30 male small-tailed Han sheep (4.5 months old) were randomly assigned to two groups. A basal diet with 3.4 g·sheep−1·d−1 WEA supplementation (WEA group) or with no WEA supplementation (CON group) was fed to sheep, respectively. Subcutaneous and visceral adipose tissue samples were collected during slaughter after 75-day feeding experiment. We obtained 30 differentially expressed miRNAs (DE-miRNAs) after application of WEA in diet of sheep in subcutaneous adipose tissue. Likewise, 15 DE-miRNAs in visceral adipose tissue were identified. A total of 746 differentially expressed mRNAs (DE-mRNAs) with 90 lipid-related DE-mRNAs in subcutaneous adipose tissue and 569 DE-mRNAs with 73 lipid-related DE-mRNAs in visceral adipose tissue were identified after WEA supplementation. The intersection genes from predicted target genes of DE-miRNAs by databases and actual DE-mRNAs by mRNA sequencing were enriched in several lipid-related pathways both in subcutaneous adipose and in visceral adipose tissue. We constructed differential miRNAs-mRNAs networks by Cytoscape software. Our results will help to explain regulatory mechanism of fatty acid composition in mutton by dietary WEA and will provide a theoretical basis for rational use of the plant extract in diet of sheep.HIGHLIGHTS Utilisation of Allium mongolicum Regel water extract led to alterations of endogenous miRNA and gene expression profiles in subcutaneous and visceral adipose tissue of sheep. Allium mongolicum Regel water extract improved nutritional value and flavour of mutton by changing fatty acid composition in adipose tissue. Establishment of miRNA-mRNA regulatory network with regard to manipulation of fatty acid composition in mutton by Allium mongolicum Regel water extract, and provided a theoretical basis for rational utilisation of this plant extract in diet of sheep. |
| format | Article |
| id | doaj-art-68dc0f9c720a4e779d43cfe7e079b73c |
| institution | Kabale University |
| issn | 1594-4077 1828-051X |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Italian Journal of Animal Science |
| spelling | doaj-art-68dc0f9c720a4e779d43cfe7e079b73c2025-08-20T03:31:10ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2023-12-012211120113310.1080/1828051X.2023.22227432222743Analysis of fatty acid composition and miRNA profile in adipose tissue after application of Allium mongolicum Regel extract in diet of sheepZejun Fan0Changjin Ao1Khas Erdene2Chen Bai3Ruli Sa4Qina Cao5Yankai Zheng6College of Animal Science, Inner Mongolia Agricultural University, Key Laboratory of Animal Feed and Nutrition of Inner Mongolia Autonomous RegionCollege of Animal Science, Inner Mongolia Agricultural University, Key Laboratory of Animal Feed and Nutrition of Inner Mongolia Autonomous RegionCollege of Animal Science, Inner Mongolia Agricultural University, Key Laboratory of Animal Feed and Nutrition of Inner Mongolia Autonomous RegionCollege of Animal Science, Inner Mongolia Agricultural University, Key Laboratory of Animal Feed and Nutrition of Inner Mongolia Autonomous RegionCollege of Animal Science, Inner Mongolia Agricultural University, Key Laboratory of Animal Feed and Nutrition of Inner Mongolia Autonomous RegionCollege of Animal Science, Inner Mongolia Agricultural University, Key Laboratory of Animal Feed and Nutrition of Inner Mongolia Autonomous RegionCollege of Animal Science, Inner Mongolia Agricultural University, Key Laboratory of Animal Feed and Nutrition of Inner Mongolia Autonomous RegionExtensive regulation of microRNAs (miRNAs) that belong to a type of endogenous small noncoding RNAs on gene expression in sheep has been recognised. Meanwhile, utilisation of water extract of Allium mongolicum Regel (WEA) in diet of sheep has been proved to regulate fatty acid composition and flavour in mutton. However, the regulatory mechanism remains unclear. We conducted miRNA and mRNA sequencing in this study. Briefly, 30 male small-tailed Han sheep (4.5 months old) were randomly assigned to two groups. A basal diet with 3.4 g·sheep−1·d−1 WEA supplementation (WEA group) or with no WEA supplementation (CON group) was fed to sheep, respectively. Subcutaneous and visceral adipose tissue samples were collected during slaughter after 75-day feeding experiment. We obtained 30 differentially expressed miRNAs (DE-miRNAs) after application of WEA in diet of sheep in subcutaneous adipose tissue. Likewise, 15 DE-miRNAs in visceral adipose tissue were identified. A total of 746 differentially expressed mRNAs (DE-mRNAs) with 90 lipid-related DE-mRNAs in subcutaneous adipose tissue and 569 DE-mRNAs with 73 lipid-related DE-mRNAs in visceral adipose tissue were identified after WEA supplementation. The intersection genes from predicted target genes of DE-miRNAs by databases and actual DE-mRNAs by mRNA sequencing were enriched in several lipid-related pathways both in subcutaneous adipose and in visceral adipose tissue. We constructed differential miRNAs-mRNAs networks by Cytoscape software. Our results will help to explain regulatory mechanism of fatty acid composition in mutton by dietary WEA and will provide a theoretical basis for rational use of the plant extract in diet of sheep.HIGHLIGHTS Utilisation of Allium mongolicum Regel water extract led to alterations of endogenous miRNA and gene expression profiles in subcutaneous and visceral adipose tissue of sheep. Allium mongolicum Regel water extract improved nutritional value and flavour of mutton by changing fatty acid composition in adipose tissue. Establishment of miRNA-mRNA regulatory network with regard to manipulation of fatty acid composition in mutton by Allium mongolicum Regel water extract, and provided a theoretical basis for rational utilisation of this plant extract in diet of sheep.http://dx.doi.org/10.1080/1828051X.2023.2222743plant extract supplementationmirnamrnafatty acidadipose tissue |
| spellingShingle | Zejun Fan Changjin Ao Khas Erdene Chen Bai Ruli Sa Qina Cao Yankai Zheng Analysis of fatty acid composition and miRNA profile in adipose tissue after application of Allium mongolicum Regel extract in diet of sheep Italian Journal of Animal Science plant extract supplementation mirna mrna fatty acid adipose tissue |
| title | Analysis of fatty acid composition and miRNA profile in adipose tissue after application of Allium mongolicum Regel extract in diet of sheep |
| title_full | Analysis of fatty acid composition and miRNA profile in adipose tissue after application of Allium mongolicum Regel extract in diet of sheep |
| title_fullStr | Analysis of fatty acid composition and miRNA profile in adipose tissue after application of Allium mongolicum Regel extract in diet of sheep |
| title_full_unstemmed | Analysis of fatty acid composition and miRNA profile in adipose tissue after application of Allium mongolicum Regel extract in diet of sheep |
| title_short | Analysis of fatty acid composition and miRNA profile in adipose tissue after application of Allium mongolicum Regel extract in diet of sheep |
| title_sort | analysis of fatty acid composition and mirna profile in adipose tissue after application of allium mongolicum regel extract in diet of sheep |
| topic | plant extract supplementation mirna mrna fatty acid adipose tissue |
| url | http://dx.doi.org/10.1080/1828051X.2023.2222743 |
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