Orofacial functions with emphasis on breathing and chewing patterns in individuals with and without molar incisor hypomineralisation: observational study protocol conducted in a dental clinic in Brazil

Introduction Molar incisor hypomineralisation (MIH) is a qualitative developmental defect of the enamel with a complex, multifactorial nature and a significant genetic component. Individuals with MIH have a compromised stomatognathic system manifested by muscle hyperactivity under postural and dynam...

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Main Authors: Ana Paula Taboada Sobral, Lara Jansiski Motta, Anna Carolina Ratto Tempestini Horliana, Kristianne Porta Santos Fernandes, Raquel Agnelli Mesquita-Ferrari, Sandra Kalil Bussadori, Amanda Rafaelly Honório Mandetta, Cinthya Cosme Gutierrez Duran, Caroline Diniz Pagani Vieira Ribeiro, Fabiana Car Pernomiam, Caroline Cristina Batista Camargo, Iara Maria Freitas Romano
Format: Article
Language:English
Published: BMJ Publishing Group 2025-06-01
Series:BMJ Open
Online Access:https://bmjopen.bmj.com/content/15/6/e101725.full
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author Ana Paula Taboada Sobral
Lara Jansiski Motta
Anna Carolina Ratto Tempestini Horliana
Kristianne Porta Santos Fernandes
Raquel Agnelli Mesquita-Ferrari
Sandra Kalil Bussadori
Amanda Rafaelly Honório Mandetta
Cinthya Cosme Gutierrez Duran
Caroline Diniz Pagani Vieira Ribeiro
Fabiana Car Pernomiam
Caroline Cristina Batista Camargo
Iara Maria Freitas Romano
author_facet Ana Paula Taboada Sobral
Lara Jansiski Motta
Anna Carolina Ratto Tempestini Horliana
Kristianne Porta Santos Fernandes
Raquel Agnelli Mesquita-Ferrari
Sandra Kalil Bussadori
Amanda Rafaelly Honório Mandetta
Cinthya Cosme Gutierrez Duran
Caroline Diniz Pagani Vieira Ribeiro
Fabiana Car Pernomiam
Caroline Cristina Batista Camargo
Iara Maria Freitas Romano
author_sort Ana Paula Taboada Sobral
collection DOAJ
description Introduction Molar incisor hypomineralisation (MIH) is a qualitative developmental defect of the enamel with a complex, multifactorial nature and a significant genetic component. Individuals with MIH have a compromised stomatognathic system manifested by muscle hyperactivity under postural and dynamic conditions. However, there is a gap in knowledge on the specific functional abnormalities that these individuals experience. Early identification and intervention, with a focus on the prevention of orofacial dysfunctions and deviations in facial growth and development, are aspects of the utmost importance. Therefore, the aim of the proposed study is to perform a comparative analysis of orofacial functions with an emphasis on breathing and chewing patterns in individuals with and without MIH. The secondary objective is to assess whether dentin hypersensitivity and the severity of MIH lesions are associated with alterations in orofacial functions.Methods and analysis Assessments will be performed using the Nordic Orofacial Test-Screening (NOT-S). Descriptive analyses will characterise the sample. The Shapiro-Wilk test will assess normality. For normally distributed data, analysis of variance and Tukey’s post hoc test will be used. For non-normal data, the Mann-Whitney U test will be applied. The χ2 test will analyse categorical variables and compare NOT-S domains between groups. Potential confounders (eg, age, sex, socioeconomic status) will be controlled through stratification or as covariates. Logistic and Poisson regressions will model associations for categorical and count-based outcomes, respectively. Statistical significance will be set at p<0.05.Ethics and dissemination This protocol has been approved by the Human Research Ethics Committee of Nove de Julho University (certificate number: 83969924.2.0000.5511; approval date: 22 November 2024). Participants will agree to take part in the study by signing an informed consent form. The findings will be published in a peer-reviewed journal. The collected data will be available on request.Trial registration number NCT06692257.
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spelling doaj-art-68db5af76b714bca8be156089e36f6272025-08-20T03:24:33ZengBMJ Publishing GroupBMJ Open2044-60552025-06-0115610.1136/bmjopen-2025-101725Orofacial functions with emphasis on breathing and chewing patterns in individuals with and without molar incisor hypomineralisation: observational study protocol conducted in a dental clinic in BrazilAna Paula Taboada Sobral0Lara Jansiski Motta1Anna Carolina Ratto Tempestini Horliana2Kristianne Porta Santos Fernandes3Raquel Agnelli Mesquita-Ferrari4Sandra Kalil Bussadori5Amanda Rafaelly Honório Mandetta6Cinthya Cosme Gutierrez Duran7Caroline Diniz Pagani Vieira Ribeiro8Fabiana Car Pernomiam9Caroline Cristina Batista Camargo10Iara Maria Freitas Romano11Postgraduation Programme in Health and Environment, Universidade Metropolitana de Santos, Santos, SP, BrazilBiophotonics Medicine Postgraduate Program, Universidade Nove de Julho - Campus Vergueiro, Sao Paulo, SP, BrazilBiophotonics Medicine Postgraduate Program, Universidade Nove de Julho - Campus Vergueiro, Sao Paulo, SP, BrazilBiophotonics Medicine Postgraduate Program, Universidade Nove de Julho - Campus Vergueiro, Sao Paulo, SP, BrazilBiophotonics Medicine Postgraduate Program, Universidade Nove de Julho - Campus Vergueiro, Sao Paulo, SP, BrazilPostgraduate Programme in Rehabilitation Sciences, Universidade Nove de Julho - Campus Vergueiro, São Paulo, SP, BrazilBiophotonics Medicine Postgraduate Program, Universidade Nove de Julho - Campus Vergueiro, Sao Paulo, SP, BrazilBiophotonics Medicine Postgraduate Program, Universidade Nove de Julho - Campus Vergueiro, Sao Paulo, SP, BrazilBiophotonics Medicine Postgraduate Program, Universidade Nove de Julho - Campus Vergueiro, Sao Paulo, SP, BrazilPostgraduate Programme in Rehabilitation Sciences, Universidade Nove de Julho - Campus Vergueiro, São Paulo, SP, BrazilPostgraduate Programme in Rehabilitation Sciences, Universidade Nove de Julho - Campus Vergueiro, São Paulo, SP, BrazilBiophotonics Medicine Postgraduate Program, Universidade Nove de Julho - Campus Vergueiro, Sao Paulo, SP, BrazilIntroduction Molar incisor hypomineralisation (MIH) is a qualitative developmental defect of the enamel with a complex, multifactorial nature and a significant genetic component. Individuals with MIH have a compromised stomatognathic system manifested by muscle hyperactivity under postural and dynamic conditions. However, there is a gap in knowledge on the specific functional abnormalities that these individuals experience. Early identification and intervention, with a focus on the prevention of orofacial dysfunctions and deviations in facial growth and development, are aspects of the utmost importance. Therefore, the aim of the proposed study is to perform a comparative analysis of orofacial functions with an emphasis on breathing and chewing patterns in individuals with and without MIH. The secondary objective is to assess whether dentin hypersensitivity and the severity of MIH lesions are associated with alterations in orofacial functions.Methods and analysis Assessments will be performed using the Nordic Orofacial Test-Screening (NOT-S). Descriptive analyses will characterise the sample. The Shapiro-Wilk test will assess normality. For normally distributed data, analysis of variance and Tukey’s post hoc test will be used. For non-normal data, the Mann-Whitney U test will be applied. The χ2 test will analyse categorical variables and compare NOT-S domains between groups. Potential confounders (eg, age, sex, socioeconomic status) will be controlled through stratification or as covariates. Logistic and Poisson regressions will model associations for categorical and count-based outcomes, respectively. Statistical significance will be set at p<0.05.Ethics and dissemination This protocol has been approved by the Human Research Ethics Committee of Nove de Julho University (certificate number: 83969924.2.0000.5511; approval date: 22 November 2024). Participants will agree to take part in the study by signing an informed consent form. The findings will be published in a peer-reviewed journal. The collected data will be available on request.Trial registration number NCT06692257.https://bmjopen.bmj.com/content/15/6/e101725.full
spellingShingle Ana Paula Taboada Sobral
Lara Jansiski Motta
Anna Carolina Ratto Tempestini Horliana
Kristianne Porta Santos Fernandes
Raquel Agnelli Mesquita-Ferrari
Sandra Kalil Bussadori
Amanda Rafaelly Honório Mandetta
Cinthya Cosme Gutierrez Duran
Caroline Diniz Pagani Vieira Ribeiro
Fabiana Car Pernomiam
Caroline Cristina Batista Camargo
Iara Maria Freitas Romano
Orofacial functions with emphasis on breathing and chewing patterns in individuals with and without molar incisor hypomineralisation: observational study protocol conducted in a dental clinic in Brazil
BMJ Open
title Orofacial functions with emphasis on breathing and chewing patterns in individuals with and without molar incisor hypomineralisation: observational study protocol conducted in a dental clinic in Brazil
title_full Orofacial functions with emphasis on breathing and chewing patterns in individuals with and without molar incisor hypomineralisation: observational study protocol conducted in a dental clinic in Brazil
title_fullStr Orofacial functions with emphasis on breathing and chewing patterns in individuals with and without molar incisor hypomineralisation: observational study protocol conducted in a dental clinic in Brazil
title_full_unstemmed Orofacial functions with emphasis on breathing and chewing patterns in individuals with and without molar incisor hypomineralisation: observational study protocol conducted in a dental clinic in Brazil
title_short Orofacial functions with emphasis on breathing and chewing patterns in individuals with and without molar incisor hypomineralisation: observational study protocol conducted in a dental clinic in Brazil
title_sort orofacial functions with emphasis on breathing and chewing patterns in individuals with and without molar incisor hypomineralisation observational study protocol conducted in a dental clinic in brazil
url https://bmjopen.bmj.com/content/15/6/e101725.full
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