Integration of Physiological and Comparative Transcriptomic Analyses Reveal the Toxicity Mechanism of <i>p</i>-Coumaric Acid on <i>Morchella importuna</i>
<i>p</i>-coumaric acid (<i>p</i>-CA) is one of the main allelochemicals of cultivable <i>Morchella</i> mushrooms. However, its toxicity mechanism has not been elucidated. Therefore, we used physiological and comparative transcriptomic analyses to reveal its toxici...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-07-01
|
| Series: | Horticulturae |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-7524/11/7/755 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | <i>p</i>-coumaric acid (<i>p</i>-CA) is one of the main allelochemicals of cultivable <i>Morchella</i> mushrooms. However, its toxicity mechanism has not been elucidated. Therefore, we used physiological and comparative transcriptomic analyses to reveal its toxicity mechanism. The results suggest that the mycelial growth and sclerotial production of <i>M. importuna</i> were promoted under treatment with a low dosage of <i>p</i>-CA (10 μg/mL). The treatment induced moderate reactive oxygen species (ROS) accumulation, with an upregulation of genes associated with antioxidant regulation, energy supply and damage repair. In contrast, oxidative stress induced under treatment with a high dosage of <i>p</i>-CA (50 μg/mL) led to strain ageing. The contents of ROS were significantly increased, along with decreased peroxidase and catalase activity. Moreover, the genes associated with H<sub>2</sub>O<sub>2</sub> synthesis were upregulated, while those responsible for H<sub>2</sub>O<sub>2</sub> decomposition, non-enzymatic antioxidant components and damage repair were downregulated. Meanwhile, the carbohydrate and lipid metabolic pathways, and the signal transduction and cell division pathways, were impaired. Taken together, moderate stress induced under a low concentration of <i>p</i>-CA promotes the mycelial growth and sclerotial metamorphosis of <i>M. importuna</i>. This study provides new insights into the potential mechanisms of continuous cropping obstacles in the cultivation of morel mushrooms, which is of great importance for the practical aspects of mushroom cultivation. |
|---|---|
| ISSN: | 2311-7524 |