Accelerated dynamic light sheet microscopy: unifying time-varying patterned illumination and low-rank and sparsity constrained reconstruction
Light Sheet Fluorescence Microscopy (LSFM) enables rapid and gentle 3D fluorescence imaging of dynamic processes over extended periods in translucent samples at the mesoscopic scale. However, its temporal resolution is constrained by the sequential acquisition of individual two-dimensional planes at...
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| Format: | Article |
| Language: | English |
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IOP Publishing
2025-01-01
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| Series: | JPhys Photonics |
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| Online Access: | https://doi.org/10.1088/2515-7647/adad23 |
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| author | Marco Tobia Vitali Alessia Candeo Andrea Farina Paolo Pozzi Alessia Brix Andrea Bassi Teresa M Correia |
| author_facet | Marco Tobia Vitali Alessia Candeo Andrea Farina Paolo Pozzi Alessia Brix Andrea Bassi Teresa M Correia |
| author_sort | Marco Tobia Vitali |
| collection | DOAJ |
| description | Light Sheet Fluorescence Microscopy (LSFM) enables rapid and gentle 3D fluorescence imaging of dynamic processes over extended periods in translucent samples at the mesoscopic scale. However, its temporal resolution is constrained by the sequential acquisition of individual two-dimensional planes at varying depths, making it challenging to capture rapid dynamics such as the beating of a zebrafish heart. To address this limitation, we recently developed spatially modulated Selective Volume Illumination Microscopy, which utilizes a compressed sensing approach to reconstruct the entire imaging volume from measurements where multiple planes are illuminated simultaneously using spatially modulated light. Building on this advancement, we now introduce a novel spatio-temporal patterned illumination strategy and volume reconstruction method that incorporates low-rank and sparsity constraints, effectively leveraging the temporal and spatial redundancy present in sequential volumetric acquisitions. This method was applied to the volumetric imaging of embryonic zebrafish hearts, achieving an improvement in imaging speed of 4-fold compared to standard LSFM and a 2-fold improvement compared to traditional compressed sensing approaches, while preserving reconstruction accuracy and enabling the visualization of fast dynamic events with a resolution of a few tens of milliseconds. Our approach represents a step forward in enhancing the temporal resolution of LSFM for studying fast biological dynamics. |
| format | Article |
| id | doaj-art-68ba35077f1544228e9d47ebf6404bb3 |
| institution | Kabale University |
| issn | 2515-7647 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | IOP Publishing |
| record_format | Article |
| series | JPhys Photonics |
| spelling | doaj-art-68ba35077f1544228e9d47ebf6404bb32025-02-10T13:15:39ZengIOP PublishingJPhys Photonics2515-76472025-01-017202500510.1088/2515-7647/adad23Accelerated dynamic light sheet microscopy: unifying time-varying patterned illumination and low-rank and sparsity constrained reconstructionMarco Tobia Vitali0https://orcid.org/0009-0002-1436-0675Alessia Candeo1https://orcid.org/0000-0001-9597-3056Andrea Farina2https://orcid.org/0000-0002-3180-6328Paolo Pozzi3https://orcid.org/0000-0002-3420-8593Alessia Brix4https://orcid.org/0000-0002-2679-9127Andrea Bassi5https://orcid.org/0000-0002-5017-0775Teresa M Correia6https://orcid.org/0000-0002-1606-9550Dipartimento di Fisica, Politecnico di Milano , Piazza Leonardo da Vinci 32, I-20133 Milano, ItalyDipartimento di Fisica, Politecnico di Milano , Piazza Leonardo da Vinci 32, I-20133 Milano, ItalyIstituto di Fotonica e Nanotecnologie, Consiglio Nazionale delle Ricerche , Piazza Leonardo da Vinci 32, I-20133 Milano, ItalyDipartimento di Fisica, Politecnico di Milano , Piazza Leonardo da Vinci 32, I-20133 Milano, ItalyDipartimento di Biotecnologie Mediche e Medicina Traslazionale, Universitá degli Studi di Milano, LITA , Via Fratelli Cervi 93, 20054 Segrate, ItalyDipartimento di Fisica, Politecnico di Milano , Piazza Leonardo da Vinci 32, I-20133 Milano, ItalyCentre of Marine Sciences (CCMAR), Universidade do Algarve , Campus de Gambelas, 8005-139 Faro, Portugal; School of Biomedical Engineering and Imaging Sciences, King’s College London , SE1 7EH London, United KingdomLight Sheet Fluorescence Microscopy (LSFM) enables rapid and gentle 3D fluorescence imaging of dynamic processes over extended periods in translucent samples at the mesoscopic scale. However, its temporal resolution is constrained by the sequential acquisition of individual two-dimensional planes at varying depths, making it challenging to capture rapid dynamics such as the beating of a zebrafish heart. To address this limitation, we recently developed spatially modulated Selective Volume Illumination Microscopy, which utilizes a compressed sensing approach to reconstruct the entire imaging volume from measurements where multiple planes are illuminated simultaneously using spatially modulated light. Building on this advancement, we now introduce a novel spatio-temporal patterned illumination strategy and volume reconstruction method that incorporates low-rank and sparsity constraints, effectively leveraging the temporal and spatial redundancy present in sequential volumetric acquisitions. This method was applied to the volumetric imaging of embryonic zebrafish hearts, achieving an improvement in imaging speed of 4-fold compared to standard LSFM and a 2-fold improvement compared to traditional compressed sensing approaches, while preserving reconstruction accuracy and enabling the visualization of fast dynamic events with a resolution of a few tens of milliseconds. Our approach represents a step forward in enhancing the temporal resolution of LSFM for studying fast biological dynamics.https://doi.org/10.1088/2515-7647/adad23light sheetlow-ranksparsitydigital micro-mirror deviceHadamard patterns |
| spellingShingle | Marco Tobia Vitali Alessia Candeo Andrea Farina Paolo Pozzi Alessia Brix Andrea Bassi Teresa M Correia Accelerated dynamic light sheet microscopy: unifying time-varying patterned illumination and low-rank and sparsity constrained reconstruction JPhys Photonics light sheet low-rank sparsity digital micro-mirror device Hadamard patterns |
| title | Accelerated dynamic light sheet microscopy: unifying time-varying patterned illumination and low-rank and sparsity constrained reconstruction |
| title_full | Accelerated dynamic light sheet microscopy: unifying time-varying patterned illumination and low-rank and sparsity constrained reconstruction |
| title_fullStr | Accelerated dynamic light sheet microscopy: unifying time-varying patterned illumination and low-rank and sparsity constrained reconstruction |
| title_full_unstemmed | Accelerated dynamic light sheet microscopy: unifying time-varying patterned illumination and low-rank and sparsity constrained reconstruction |
| title_short | Accelerated dynamic light sheet microscopy: unifying time-varying patterned illumination and low-rank and sparsity constrained reconstruction |
| title_sort | accelerated dynamic light sheet microscopy unifying time varying patterned illumination and low rank and sparsity constrained reconstruction |
| topic | light sheet low-rank sparsity digital micro-mirror device Hadamard patterns |
| url | https://doi.org/10.1088/2515-7647/adad23 |
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