Erythrocytic α-Synuclein in Parkinson’s Disease and Progressive Supranuclear Palsy—A Pilot Study
<b>Background/Objectives:</b> The current research examines the accuracy of α-synuclein in RBCs as a diagnostic biomarker for PD and PSP, despite their distinct molecular etiologies. <b>Methods:</b> We used ELISA to measure total, oligomeric, and p129-α-synuclein levels in er...
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2024-11-01
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| author | Costanza Maria Cristiani Luana Scaramuzzino Elvira Immacolata Parrotta Giovanni Cuda Aldo Quattrone Andrea Quattrone |
| author_facet | Costanza Maria Cristiani Luana Scaramuzzino Elvira Immacolata Parrotta Giovanni Cuda Aldo Quattrone Andrea Quattrone |
| author_sort | Costanza Maria Cristiani |
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| description | <b>Background/Objectives:</b> The current research examines the accuracy of α-synuclein in RBCs as a diagnostic biomarker for PD and PSP, despite their distinct molecular etiologies. <b>Methods:</b> We used ELISA to measure total, oligomeric, and p129-α-synuclein levels in erythrocytes from 8 PSP patients, 19 PD patients, and 18 healthy controls (HCs). The classification performances of RBC α-synuclein levels were investigated by receiver operator characteristic (ROC) curve. We also evaluated a possible correlation between RBC α-synuclein level and the biological and clinical features of our cohorts. <b>Results:</b> RBC total α-synuclein was higher in PSP patients compared to both PD patients and HCs, achieving good classification performance (AUC: 0.853) in distinguishing PSP patients from PD patients, with a sensitivity of 100% and a specificity of 70.6%; moreover, the levels of this biomarker positively correlated with disease severity in PSP group. Regarding oligomeric α-synuclein and p129-α-synuclein, the latter was slightly increased in RBCs from PSP patients compared to HCs, but no correlations were detected. <b>Conclusions:</b> Although these findings need to be confirmed in larger studies, our pilot work suggests that RBC total α-synuclein may represent a potential molecular biomarker for the differential diagnosis and clinical staging of PSP. |
| format | Article |
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| institution | OA Journals |
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| language | English |
| publishDate | 2024-11-01 |
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| spelling | doaj-art-68b3f58b69004191b3ff4f4ce91828db2025-08-20T02:28:07ZengMDPI AGBiomedicines2227-90592024-11-011211251010.3390/biomedicines12112510Erythrocytic α-Synuclein in Parkinson’s Disease and Progressive Supranuclear Palsy—A Pilot StudyCostanza Maria Cristiani0Luana Scaramuzzino1Elvira Immacolata Parrotta2Giovanni Cuda3Aldo Quattrone4Andrea Quattrone5Neuroscience Research Center, Department of Medical and Surgical Sciences, University “Magna Graecia”, 88100 Catanzaro, ItalyNeuroscience Research Center, Department of Medical and Surgical Sciences, University “Magna Graecia”, 88100 Catanzaro, ItalyInstitute of Molecular Biology, Department of Medical and Surgical Sciences, University “Magna Graecia”, 88100 Catanzaro, ItalyResearch Centre for Advanced Biochemistry and Molecular Biology, Department of Clinical and Experimental Medicine, University “Magna Graecia”, 88100 Catanzaro, ItalyNeuroscience Research Center, Department of Medical and Surgical Sciences, University “Magna Graecia”, 88100 Catanzaro, ItalyNeuroscience Research Center, Department of Medical and Surgical Sciences, University “Magna Graecia”, 88100 Catanzaro, Italy<b>Background/Objectives:</b> The current research examines the accuracy of α-synuclein in RBCs as a diagnostic biomarker for PD and PSP, despite their distinct molecular etiologies. <b>Methods:</b> We used ELISA to measure total, oligomeric, and p129-α-synuclein levels in erythrocytes from 8 PSP patients, 19 PD patients, and 18 healthy controls (HCs). The classification performances of RBC α-synuclein levels were investigated by receiver operator characteristic (ROC) curve. We also evaluated a possible correlation between RBC α-synuclein level and the biological and clinical features of our cohorts. <b>Results:</b> RBC total α-synuclein was higher in PSP patients compared to both PD patients and HCs, achieving good classification performance (AUC: 0.853) in distinguishing PSP patients from PD patients, with a sensitivity of 100% and a specificity of 70.6%; moreover, the levels of this biomarker positively correlated with disease severity in PSP group. Regarding oligomeric α-synuclein and p129-α-synuclein, the latter was slightly increased in RBCs from PSP patients compared to HCs, but no correlations were detected. <b>Conclusions:</b> Although these findings need to be confirmed in larger studies, our pilot work suggests that RBC total α-synuclein may represent a potential molecular biomarker for the differential diagnosis and clinical staging of PSP.https://www.mdpi.com/2227-9059/12/11/2510Parkinson’s diseaseprogressive supranuclear palsyα-synucleinoligomeric α-synucleinphosphorylated α-synucleinred blood cells |
| spellingShingle | Costanza Maria Cristiani Luana Scaramuzzino Elvira Immacolata Parrotta Giovanni Cuda Aldo Quattrone Andrea Quattrone Erythrocytic α-Synuclein in Parkinson’s Disease and Progressive Supranuclear Palsy—A Pilot Study Biomedicines Parkinson’s disease progressive supranuclear palsy α-synuclein oligomeric α-synuclein phosphorylated α-synuclein red blood cells |
| title | Erythrocytic α-Synuclein in Parkinson’s Disease and Progressive Supranuclear Palsy—A Pilot Study |
| title_full | Erythrocytic α-Synuclein in Parkinson’s Disease and Progressive Supranuclear Palsy—A Pilot Study |
| title_fullStr | Erythrocytic α-Synuclein in Parkinson’s Disease and Progressive Supranuclear Palsy—A Pilot Study |
| title_full_unstemmed | Erythrocytic α-Synuclein in Parkinson’s Disease and Progressive Supranuclear Palsy—A Pilot Study |
| title_short | Erythrocytic α-Synuclein in Parkinson’s Disease and Progressive Supranuclear Palsy—A Pilot Study |
| title_sort | erythrocytic α synuclein in parkinson s disease and progressive supranuclear palsy a pilot study |
| topic | Parkinson’s disease progressive supranuclear palsy α-synuclein oligomeric α-synuclein phosphorylated α-synuclein red blood cells |
| url | https://www.mdpi.com/2227-9059/12/11/2510 |
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