Impacts of sulfate polysaccharide JCS1S2 on retinal neovascularization in oxygen-induced retinopathy rats
Retinal neovascularization, a pathological form of angiogenesis, is a leading cause of blindness. This study investigated the effects of Dendrobium sulfate polysaccharide JCS1S2, derived from Dendrobium chrysogenum, on oxygen-induced retinopathy (OIR) in rat models and Müller cells of the rat retina...
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Frontiers Media S.A.
2025-04-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2025.1499420/full |
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| author | Yang Bai Rui Wang Can Jin Can Jin Lili Wang Yun Tang Han Wang Kan Ding Kan Ding Shanjun Cai |
| author_facet | Yang Bai Rui Wang Can Jin Can Jin Lili Wang Yun Tang Han Wang Kan Ding Kan Ding Shanjun Cai |
| author_sort | Yang Bai |
| collection | DOAJ |
| description | Retinal neovascularization, a pathological form of angiogenesis, is a leading cause of blindness. This study investigated the effects of Dendrobium sulfate polysaccharide JCS1S2, derived from Dendrobium chrysogenum, on oxygen-induced retinopathy (OIR) in rat models and Müller cells of the rat retina. To this end, we established an OIR rat model and divided the rats into three primary groups, namely, Group I (control), Group II (OIR), and Group III (OIR + JCS1S2). Group III was further subdivided into three subgroups treated with different concentrations of JCS1S2 (10 μg/μL, 20 μg/μL, and 40 μg/μL). After finding the optimal concentration of JCS1S2 by ADP and HE, PCR, Western blot and transcriptome sequencing were used to analyze the role of JCS1S2 in Müller cells of OIR rats and rat retinas. ADP and hematoxylin and eosin (HE) staining revealed that JCS1S2 dose-dependently inhibited retinal neovascularization. Quantitative polymerase chain reaction (qPCR) and Western blot analyses showed significant downregulation of vascular endothelial growth factor A (VEGF-A), VEGF-B, VEGF-D, VEGF receptor 1 (VEGF-R1), and VEGF receptor 2 (VEGF-R2) following JCS1S2 treatment. Transcriptome analysis suggested that JCS1S2 may suppress the activation of the Toll-like receptor (TLR) signaling pathway, regulate the expression of genes associated with endothelial activation and angiogenesis, and participate in the inflammatory and metabolic pathways of the retina. Western blotting data indicate that JCS1S2 can markedly reduce abnormal retinal angiogenesis and Müller cell activation in OIR rats through the TLR4/p-NF-κB/VEGF pathway, JCS1S2 may have the potential as a therapeutic agent for retinal neovascularization. |
| format | Article |
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| institution | OA Journals |
| issn | 1663-9812 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Pharmacology |
| spelling | doaj-art-68b31005123740f8a2682432bfc158012025-08-20T02:16:29ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-04-011610.3389/fphar.2025.14994201499420Impacts of sulfate polysaccharide JCS1S2 on retinal neovascularization in oxygen-induced retinopathy ratsYang Bai0Rui Wang1Can Jin2Can Jin3Lili Wang4Yun Tang5Han Wang6Kan Ding7Kan Ding8Shanjun Cai9Department of Ophthalmology, Special Key Laboratory of Ocular Diseases of Guizhou Province, Affiliated Hospital of Zunyi Medical University, Guizhou Eye Hospital, Guizhou Provincial Branch of National Eye Disease Clinical Research Center, Zunyi Medical University, Zunyi, Guizhou Province, ChinaDepartment of Ophthalmology, Special Key Laboratory of Ocular Diseases of Guizhou Province, Affiliated Hospital of Zunyi Medical University, Guizhou Eye Hospital, Guizhou Provincial Branch of National Eye Disease Clinical Research Center, Zunyi Medical University, Zunyi, Guizhou Province, ChinaZhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica, Chinese Academy of Science, Zhongshan, ChinaGlycochemistry and Glycobiology Lab, CAS Key Laboratory of Receptor Research, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Beijing, ChinaDepartment of Ophthalmology, Special Key Laboratory of Ocular Diseases of Guizhou Province, Affiliated Hospital of Zunyi Medical University, Guizhou Eye Hospital, Guizhou Provincial Branch of National Eye Disease Clinical Research Center, Zunyi Medical University, Zunyi, Guizhou Province, ChinaDepartment of Ophthalmology, Special Key Laboratory of Ocular Diseases of Guizhou Province, Affiliated Hospital of Zunyi Medical University, Guizhou Eye Hospital, Guizhou Provincial Branch of National Eye Disease Clinical Research Center, Zunyi Medical University, Zunyi, Guizhou Province, ChinaDepartment of Ophthalmology, Special Key Laboratory of Ocular Diseases of Guizhou Province, Affiliated Hospital of Zunyi Medical University, Guizhou Eye Hospital, Guizhou Provincial Branch of National Eye Disease Clinical Research Center, Zunyi Medical University, Zunyi, Guizhou Province, ChinaZhongshan Institute for Drug Discovery, Shanghai Institute of Materia Medica, Chinese Academy of Science, Zhongshan, ChinaGlycochemistry and Glycobiology Lab, CAS Key Laboratory of Receptor Research, State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Beijing, ChinaDepartment of Ophthalmology, Special Key Laboratory of Ocular Diseases of Guizhou Province, Affiliated Hospital of Zunyi Medical University, Guizhou Eye Hospital, Guizhou Provincial Branch of National Eye Disease Clinical Research Center, Zunyi Medical University, Zunyi, Guizhou Province, ChinaRetinal neovascularization, a pathological form of angiogenesis, is a leading cause of blindness. This study investigated the effects of Dendrobium sulfate polysaccharide JCS1S2, derived from Dendrobium chrysogenum, on oxygen-induced retinopathy (OIR) in rat models and Müller cells of the rat retina. To this end, we established an OIR rat model and divided the rats into three primary groups, namely, Group I (control), Group II (OIR), and Group III (OIR + JCS1S2). Group III was further subdivided into three subgroups treated with different concentrations of JCS1S2 (10 μg/μL, 20 μg/μL, and 40 μg/μL). After finding the optimal concentration of JCS1S2 by ADP and HE, PCR, Western blot and transcriptome sequencing were used to analyze the role of JCS1S2 in Müller cells of OIR rats and rat retinas. ADP and hematoxylin and eosin (HE) staining revealed that JCS1S2 dose-dependently inhibited retinal neovascularization. Quantitative polymerase chain reaction (qPCR) and Western blot analyses showed significant downregulation of vascular endothelial growth factor A (VEGF-A), VEGF-B, VEGF-D, VEGF receptor 1 (VEGF-R1), and VEGF receptor 2 (VEGF-R2) following JCS1S2 treatment. Transcriptome analysis suggested that JCS1S2 may suppress the activation of the Toll-like receptor (TLR) signaling pathway, regulate the expression of genes associated with endothelial activation and angiogenesis, and participate in the inflammatory and metabolic pathways of the retina. Western blotting data indicate that JCS1S2 can markedly reduce abnormal retinal angiogenesis and Müller cell activation in OIR rats through the TLR4/p-NF-κB/VEGF pathway, JCS1S2 may have the potential as a therapeutic agent for retinal neovascularization.https://www.frontiersin.org/articles/10.3389/fphar.2025.1499420/fullDendrobium nobile Lindlpolysaccharide sulfate JCS1S2retinaoxygen-induced retinopathyvascular endothelial growth factor |
| spellingShingle | Yang Bai Rui Wang Can Jin Can Jin Lili Wang Yun Tang Han Wang Kan Ding Kan Ding Shanjun Cai Impacts of sulfate polysaccharide JCS1S2 on retinal neovascularization in oxygen-induced retinopathy rats Frontiers in Pharmacology Dendrobium nobile Lindl polysaccharide sulfate JCS1S2 retina oxygen-induced retinopathy vascular endothelial growth factor |
| title | Impacts of sulfate polysaccharide JCS1S2 on retinal neovascularization in oxygen-induced retinopathy rats |
| title_full | Impacts of sulfate polysaccharide JCS1S2 on retinal neovascularization in oxygen-induced retinopathy rats |
| title_fullStr | Impacts of sulfate polysaccharide JCS1S2 on retinal neovascularization in oxygen-induced retinopathy rats |
| title_full_unstemmed | Impacts of sulfate polysaccharide JCS1S2 on retinal neovascularization in oxygen-induced retinopathy rats |
| title_short | Impacts of sulfate polysaccharide JCS1S2 on retinal neovascularization in oxygen-induced retinopathy rats |
| title_sort | impacts of sulfate polysaccharide jcs1s2 on retinal neovascularization in oxygen induced retinopathy rats |
| topic | Dendrobium nobile Lindl polysaccharide sulfate JCS1S2 retina oxygen-induced retinopathy vascular endothelial growth factor |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2025.1499420/full |
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