Extraction, Characterization, and Molecular Weight Determination of Senna tora (L.) Seed Polysaccharide
The objective of the present work was extraction of polysaccharide from Senna tora L. seed and its characterization as a pharmaceutical excipient. Polysaccharide extraction was based on mechanical separation of the endosperm of seeds of Senna tora, water dissolution, centrifugation, and precipitatio...
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Wiley
2015-01-01
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| Series: | International Journal of Biomaterials |
| Online Access: | http://dx.doi.org/10.1155/2015/928679 |
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| author | Harshal A. Pawar K. G. Lalitha |
| author_facet | Harshal A. Pawar K. G. Lalitha |
| author_sort | Harshal A. Pawar |
| collection | DOAJ |
| description | The objective of the present work was extraction of polysaccharide from Senna tora L. seed and its characterization as a pharmaceutical excipient. Polysaccharide extraction was based on mechanical separation of the endosperm of seeds of Senna tora, water dissolution, centrifugation, and precipitation with acetone. Standard procedures were used to study the viscosity, micromeritic properties, and microbial bioburden. Accelerated stability study was carried out on isolated polysaccharide for six months at 40°C/75 RH as per ICH guidelines. The gum obtained from S. tora seeds was an amorphous free flowing odourless powder with dull brown colour (yield = 35% w/w). The bulk density, tapped density, and angle of repose data reveal that S. tora gum possesses good flow property. The intrinsic viscosity obtained was 1.568 dL/g. The average molecular weight of purified S. tora gum was found to be 198 kDa by intrinsic viscosity method. The results indicated that viscosity of gum solution increases with increase in temperature. FTIR study revealed the absence of degradation or decomposition of polysaccharide at accelerated stability conditions for six months. It has been concluded that extracted polysaccharide can be used as pharmaceutical excipient in terms of flow behavior, microbial properties, and stability. |
| format | Article |
| id | doaj-art-68a9c9c6f4354ac0868c9bc66aecdee2 |
| institution | Kabale University |
| issn | 1687-8787 1687-8795 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
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| series | International Journal of Biomaterials |
| spelling | doaj-art-68a9c9c6f4354ac0868c9bc66aecdee22025-02-03T05:53:46ZengWileyInternational Journal of Biomaterials1687-87871687-87952015-01-01201510.1155/2015/928679928679Extraction, Characterization, and Molecular Weight Determination of Senna tora (L.) Seed PolysaccharideHarshal A. Pawar0K. G. Lalitha1Department of Quality Assurance, Dr. L. H. Hiranandani College of Pharmacy, Ulhasnagar, Maharashtra 421003, IndiaDepartment of Pharmaceutical Chemistry, Ultra College of Pharmacy, No. 4/235, College Road, Thasildar Nagar, Madurai, Tamil Nadu 625020, IndiaThe objective of the present work was extraction of polysaccharide from Senna tora L. seed and its characterization as a pharmaceutical excipient. Polysaccharide extraction was based on mechanical separation of the endosperm of seeds of Senna tora, water dissolution, centrifugation, and precipitation with acetone. Standard procedures were used to study the viscosity, micromeritic properties, and microbial bioburden. Accelerated stability study was carried out on isolated polysaccharide for six months at 40°C/75 RH as per ICH guidelines. The gum obtained from S. tora seeds was an amorphous free flowing odourless powder with dull brown colour (yield = 35% w/w). The bulk density, tapped density, and angle of repose data reveal that S. tora gum possesses good flow property. The intrinsic viscosity obtained was 1.568 dL/g. The average molecular weight of purified S. tora gum was found to be 198 kDa by intrinsic viscosity method. The results indicated that viscosity of gum solution increases with increase in temperature. FTIR study revealed the absence of degradation or decomposition of polysaccharide at accelerated stability conditions for six months. It has been concluded that extracted polysaccharide can be used as pharmaceutical excipient in terms of flow behavior, microbial properties, and stability.http://dx.doi.org/10.1155/2015/928679 |
| spellingShingle | Harshal A. Pawar K. G. Lalitha Extraction, Characterization, and Molecular Weight Determination of Senna tora (L.) Seed Polysaccharide International Journal of Biomaterials |
| title | Extraction, Characterization, and Molecular Weight Determination of Senna tora (L.) Seed Polysaccharide |
| title_full | Extraction, Characterization, and Molecular Weight Determination of Senna tora (L.) Seed Polysaccharide |
| title_fullStr | Extraction, Characterization, and Molecular Weight Determination of Senna tora (L.) Seed Polysaccharide |
| title_full_unstemmed | Extraction, Characterization, and Molecular Weight Determination of Senna tora (L.) Seed Polysaccharide |
| title_short | Extraction, Characterization, and Molecular Weight Determination of Senna tora (L.) Seed Polysaccharide |
| title_sort | extraction characterization and molecular weight determination of senna tora l seed polysaccharide |
| url | http://dx.doi.org/10.1155/2015/928679 |
| work_keys_str_mv | AT harshalapawar extractioncharacterizationandmolecularweightdeterminationofsennatoralseedpolysaccharide AT kglalitha extractioncharacterizationandmolecularweightdeterminationofsennatoralseedpolysaccharide |