Structure of an F-type phage tail-like bacteriocin from Listeria monocytogenes
Abstract F-type phage tail-like bacteriocins (PTLBs) are high-molecular-weight protein complexes exhibiting bactericidal activity and share evolutionary similarities with the tails of non-contractile siphoviruses. In this study, we present the atomic structure of monocin, a genetically engineered F-...
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| Format: | Article |
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Nature Portfolio
2025-02-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-57075-3 |
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| _version_ | 1849724070108594176 |
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| author | Zhiwei Gu Xiaofei Ge Jiawei Wang |
| author_facet | Zhiwei Gu Xiaofei Ge Jiawei Wang |
| author_sort | Zhiwei Gu |
| collection | DOAJ |
| description | Abstract F-type phage tail-like bacteriocins (PTLBs) are high-molecular-weight protein complexes exhibiting bactericidal activity and share evolutionary similarities with the tails of non-contractile siphoviruses. In this study, we present the atomic structure of monocin, a genetically engineered F-type PTLB from Listeria monocytogenes. Our detailed atomic-level analysis, excluding two chaperone proteins, provides crucial insights into the molecular architecture of F-type PTLBs. The core structure of monocin resembles TP901-1-like phage tails, featuring three side fibers with receptor-binding domains that connect to the baseplate for host adhesion. Based on these findings, we propose a potential mechanism by which F-type PTLBs induce cell death, offering a foundation for developing targeted antibacterial therapies. |
| format | Article |
| id | doaj-art-68a8bb84fc344736aeb545d41336e4bf |
| institution | DOAJ |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-68a8bb84fc344736aeb545d41336e4bf2025-08-20T03:10:50ZengNature PortfolioNature Communications2041-17232025-02-011611910.1038/s41467-025-57075-3Structure of an F-type phage tail-like bacteriocin from Listeria monocytogenesZhiwei Gu0Xiaofei Ge1Jiawei Wang2State Key Laboratory of Membrane Biology, Beijing Frontier Research Center for Biological Structure, School of Life Sciences, Tsinghua UniversityHealth and Wellness, City University of MacauState Key Laboratory of Membrane Biology, Beijing Frontier Research Center for Biological Structure, School of Life Sciences, Tsinghua UniversityAbstract F-type phage tail-like bacteriocins (PTLBs) are high-molecular-weight protein complexes exhibiting bactericidal activity and share evolutionary similarities with the tails of non-contractile siphoviruses. In this study, we present the atomic structure of monocin, a genetically engineered F-type PTLB from Listeria monocytogenes. Our detailed atomic-level analysis, excluding two chaperone proteins, provides crucial insights into the molecular architecture of F-type PTLBs. The core structure of monocin resembles TP901-1-like phage tails, featuring three side fibers with receptor-binding domains that connect to the baseplate for host adhesion. Based on these findings, we propose a potential mechanism by which F-type PTLBs induce cell death, offering a foundation for developing targeted antibacterial therapies.https://doi.org/10.1038/s41467-025-57075-3 |
| spellingShingle | Zhiwei Gu Xiaofei Ge Jiawei Wang Structure of an F-type phage tail-like bacteriocin from Listeria monocytogenes Nature Communications |
| title | Structure of an F-type phage tail-like bacteriocin from Listeria monocytogenes |
| title_full | Structure of an F-type phage tail-like bacteriocin from Listeria monocytogenes |
| title_fullStr | Structure of an F-type phage tail-like bacteriocin from Listeria monocytogenes |
| title_full_unstemmed | Structure of an F-type phage tail-like bacteriocin from Listeria monocytogenes |
| title_short | Structure of an F-type phage tail-like bacteriocin from Listeria monocytogenes |
| title_sort | structure of an f type phage tail like bacteriocin from listeria monocytogenes |
| url | https://doi.org/10.1038/s41467-025-57075-3 |
| work_keys_str_mv | AT zhiweigu structureofanftypephagetaillikebacteriocinfromlisteriamonocytogenes AT xiaofeige structureofanftypephagetaillikebacteriocinfromlisteriamonocytogenes AT jiaweiwang structureofanftypephagetaillikebacteriocinfromlisteriamonocytogenes |