Subspecies distribution and drug-resistance characteristics of Mycobacterium abscessus complex clinical isolates in South China
ABSTRACT Mycobacterium abscessus complex (MABC) is the most common rapidly growing non-tuberculous mycobacterium (NTM) and a significant pathogen responsible for various infections. Subspecies of MABC exhibit differing levels of antibiotic resistance, potentially influencing patient prognosis. In th...
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American Society for Microbiology
2025-05-01
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| Series: | Microbiology Spectrum |
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| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.04103-23 |
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| author | Xinyu Wang Biyi Su Pinru Chen Haobin Kuang Ping Guan Chao Zhang Liping Pan Jialou Zhu Yaoju Tan |
| author_facet | Xinyu Wang Biyi Su Pinru Chen Haobin Kuang Ping Guan Chao Zhang Liping Pan Jialou Zhu Yaoju Tan |
| author_sort | Xinyu Wang |
| collection | DOAJ |
| description | ABSTRACT Mycobacterium abscessus complex (MABC) is the most common rapidly growing non-tuberculous mycobacterium (NTM) and a significant pathogen responsible for various infections. Subspecies of MABC exhibit differing levels of antibiotic resistance, potentially influencing patient prognosis. In this study, 196 MABC clinical isolates were collected from Guangzhou Chest Hospital and subjected to a drug sensitivity test (DST) followed by whole-genome sequencing. In our study, the most common subspecies was Mycobacterium abscessus subsp. abscessus, followed by M. abscessus subsp. massiliense, with M. abscessus subsp. bolletii being the least frequent. The isolates were most susceptible to tigecycline and amikacin, followed by clarithromycin and linezolid. Bedaquiline and clofazimine showed promising anti-bacterial activity, whereas doramapimod and capreomycin demonstrated limited efficacy. The erm(41) gene mutation at position 28 and the rrl gene at position 2270 were identified in clarithromycin-resistant isolates. Additionally, the rrs gene mutation at position 1375 may serve as an indicator of amikacin resistance. Regular molecular surveillance investigation of NTM and DST is recommended for improved management.IMPORTANCEAs Guangdong is a region with a high prevalence of NTM, this study investigates the epidemiological trends of MABC subspecies and their associated drug-resistance profiles, addressing a critical research gap. The findings are crucial for guiding hospital drug management and clinical decision making, enabling more accurate diagnoses and the formulation of personalized, effective treatment plans. Additionally, this study provides a valuable reference for the development of drug-resistance detection reagents and new anti-bacterial agents. |
| format | Article |
| id | doaj-art-68a229b554ea4f1cab301ffe34c689e0 |
| institution | OA Journals |
| issn | 2165-0497 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj-art-68a229b554ea4f1cab301ffe34c689e02025-08-20T02:14:57ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972025-05-0113510.1128/spectrum.04103-23Subspecies distribution and drug-resistance characteristics of Mycobacterium abscessus complex clinical isolates in South ChinaXinyu Wang0Biyi Su1Pinru Chen2Haobin Kuang3Ping Guan4Chao Zhang5Liping Pan6Jialou Zhu7Yaoju Tan8State Key Laboratory of Respiratory Disease, Guangzhou Key Laboratory of Tuberculosis Research, Department of Clinical Laboratory, Guangzhou Chest Hospital, Institute of Tuberculosis, Guangzhou Medical University, Guangzhou, Guangdong, ChinaState Key Laboratory of Respiratory Disease, Guangzhou Key Laboratory of Tuberculosis Research, Department of Clinical Laboratory, Guangzhou Chest Hospital, Institute of Tuberculosis, Guangzhou Medical University, Guangzhou, Guangdong, ChinaState Key Laboratory of Respiratory Disease, Guangzhou Key Laboratory of Tuberculosis Research, Department of Tuberculosis, Guangzhou Chest Hospital, Institute of Tuberculosis, Guangzhou Medical University, Guangzhou, Guangdong, ChinaState Key Laboratory of Respiratory Disease, Guangzhou Key Laboratory of Tuberculosis Research, Department of Tuberculosis, Guangzhou Chest Hospital, Institute of Tuberculosis, Guangzhou Medical University, Guangzhou, Guangdong, ChinaState Key Laboratory of Respiratory Disease, Guangzhou Key Laboratory of Tuberculosis Research, Department of Clinical Laboratory, Guangzhou Chest Hospital, Institute of Tuberculosis, Guangzhou Medical University, Guangzhou, Guangdong, ChinaBeijing Key Laboratory for Drug Resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing, ChinaBeijing Key Laboratory for Drug Resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute, Beijing, ChinaState Key Laboratory of Respiratory Disease, Guangzhou Key Laboratory of Tuberculosis Research, Department of Clinical Laboratory, Guangzhou Chest Hospital, Institute of Tuberculosis, Guangzhou Medical University, Guangzhou, Guangdong, ChinaState Key Laboratory of Respiratory Disease, Guangzhou Key Laboratory of Tuberculosis Research, Department of Clinical Laboratory, Guangzhou Chest Hospital, Institute of Tuberculosis, Guangzhou Medical University, Guangzhou, Guangdong, ChinaABSTRACT Mycobacterium abscessus complex (MABC) is the most common rapidly growing non-tuberculous mycobacterium (NTM) and a significant pathogen responsible for various infections. Subspecies of MABC exhibit differing levels of antibiotic resistance, potentially influencing patient prognosis. In this study, 196 MABC clinical isolates were collected from Guangzhou Chest Hospital and subjected to a drug sensitivity test (DST) followed by whole-genome sequencing. In our study, the most common subspecies was Mycobacterium abscessus subsp. abscessus, followed by M. abscessus subsp. massiliense, with M. abscessus subsp. bolletii being the least frequent. The isolates were most susceptible to tigecycline and amikacin, followed by clarithromycin and linezolid. Bedaquiline and clofazimine showed promising anti-bacterial activity, whereas doramapimod and capreomycin demonstrated limited efficacy. The erm(41) gene mutation at position 28 and the rrl gene at position 2270 were identified in clarithromycin-resistant isolates. Additionally, the rrs gene mutation at position 1375 may serve as an indicator of amikacin resistance. Regular molecular surveillance investigation of NTM and DST is recommended for improved management.IMPORTANCEAs Guangdong is a region with a high prevalence of NTM, this study investigates the epidemiological trends of MABC subspecies and their associated drug-resistance profiles, addressing a critical research gap. The findings are crucial for guiding hospital drug management and clinical decision making, enabling more accurate diagnoses and the formulation of personalized, effective treatment plans. Additionally, this study provides a valuable reference for the development of drug-resistance detection reagents and new anti-bacterial agents.https://journals.asm.org/doi/10.1128/spectrum.04103-23Mycobacterium abscessuswhole-genome sequencingmutationdrug resistancesingle-nucleotide polymorphism |
| spellingShingle | Xinyu Wang Biyi Su Pinru Chen Haobin Kuang Ping Guan Chao Zhang Liping Pan Jialou Zhu Yaoju Tan Subspecies distribution and drug-resistance characteristics of Mycobacterium abscessus complex clinical isolates in South China Microbiology Spectrum Mycobacterium abscessus whole-genome sequencing mutation drug resistance single-nucleotide polymorphism |
| title | Subspecies distribution and drug-resistance characteristics of Mycobacterium abscessus complex clinical isolates in South China |
| title_full | Subspecies distribution and drug-resistance characteristics of Mycobacterium abscessus complex clinical isolates in South China |
| title_fullStr | Subspecies distribution and drug-resistance characteristics of Mycobacterium abscessus complex clinical isolates in South China |
| title_full_unstemmed | Subspecies distribution and drug-resistance characteristics of Mycobacterium abscessus complex clinical isolates in South China |
| title_short | Subspecies distribution and drug-resistance characteristics of Mycobacterium abscessus complex clinical isolates in South China |
| title_sort | subspecies distribution and drug resistance characteristics of mycobacterium abscessus complex clinical isolates in south china |
| topic | Mycobacterium abscessus whole-genome sequencing mutation drug resistance single-nucleotide polymorphism |
| url | https://journals.asm.org/doi/10.1128/spectrum.04103-23 |
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