Direct measurement and optimization of the polarization-dependent modulation depth in super-resolution structured illumination microscopy
Maintaining the s-polarization state of laser beams is important to achieve high modulation depth in a laser-interference-based super-resolution structured illumination microscope (SR-SIM). However, the imperfect optical components can depolarize the laser beams hence degenerating the modulation dep...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
World Scientific Publishing
2025-07-01
|
| Series: | Journal of Innovative Optical Health Sciences |
| Subjects: | |
| Online Access: | https://www.worldscientific.com/doi/10.1142/S1793545825500051 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850253830033244160 |
|---|---|
| author | Linbo Wang Simin Li Xiaohu Chen Xin Jin Jie Zhang Hui Li Gang Wen |
| author_facet | Linbo Wang Simin Li Xiaohu Chen Xin Jin Jie Zhang Hui Li Gang Wen |
| author_sort | Linbo Wang |
| collection | DOAJ |
| description | Maintaining the s-polarization state of laser beams is important to achieve high modulation depth in a laser-interference-based super-resolution structured illumination microscope (SR-SIM). However, the imperfect optical components can depolarize the laser beams hence degenerating the modulation depth. Here, we first presented a direct measurement method designed to estimate the modulation depth more precisely by shifting illumination patterns with equal phase steps. This measurement method greatly reduces the dependence of modulation depths on the samples, and then developed a polarization optimization method to achieve high modulation depth at all orientations by actively and quantitatively compensating for the additional phase difference using a combination of waveplate and a liquid crystal variable retarder (LCVR). Experimental results demonstrate that our method can achieve illumination patterns with modulation depth higher than 0.94 at three orientations with only one LCVR voltage, which enables isotropic resolution improvement. |
| format | Article |
| id | doaj-art-689077b426f54f299207571aa76d3fbf |
| institution | OA Journals |
| issn | 1793-5458 1793-7205 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | World Scientific Publishing |
| record_format | Article |
| series | Journal of Innovative Optical Health Sciences |
| spelling | doaj-art-689077b426f54f299207571aa76d3fbf2025-08-20T01:57:16ZengWorld Scientific PublishingJournal of Innovative Optical Health Sciences1793-54581793-72052025-07-01180410.1142/S1793545825500051Direct measurement and optimization of the polarization-dependent modulation depth in super-resolution structured illumination microscopyLinbo Wang0Simin Li1Xiaohu Chen2Xin Jin3Jie Zhang4Hui Li5Gang Wen6School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230026, P. R. ChinaCollege of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Jinan 250014, P. R. ChinaSuzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, P. R. ChinaSuzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, P. R. ChinaSuzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, P. R. ChinaSchool of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230026, P. R. ChinaSchool of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230026, P. R. ChinaMaintaining the s-polarization state of laser beams is important to achieve high modulation depth in a laser-interference-based super-resolution structured illumination microscope (SR-SIM). However, the imperfect optical components can depolarize the laser beams hence degenerating the modulation depth. Here, we first presented a direct measurement method designed to estimate the modulation depth more precisely by shifting illumination patterns with equal phase steps. This measurement method greatly reduces the dependence of modulation depths on the samples, and then developed a polarization optimization method to achieve high modulation depth at all orientations by actively and quantitatively compensating for the additional phase difference using a combination of waveplate and a liquid crystal variable retarder (LCVR). Experimental results demonstrate that our method can achieve illumination patterns with modulation depth higher than 0.94 at three orientations with only one LCVR voltage, which enables isotropic resolution improvement.https://www.worldscientific.com/doi/10.1142/S1793545825500051Structured illumination microscopydepolarizationmodulation depthphase compensation |
| spellingShingle | Linbo Wang Simin Li Xiaohu Chen Xin Jin Jie Zhang Hui Li Gang Wen Direct measurement and optimization of the polarization-dependent modulation depth in super-resolution structured illumination microscopy Journal of Innovative Optical Health Sciences Structured illumination microscopy depolarization modulation depth phase compensation |
| title | Direct measurement and optimization of the polarization-dependent modulation depth in super-resolution structured illumination microscopy |
| title_full | Direct measurement and optimization of the polarization-dependent modulation depth in super-resolution structured illumination microscopy |
| title_fullStr | Direct measurement and optimization of the polarization-dependent modulation depth in super-resolution structured illumination microscopy |
| title_full_unstemmed | Direct measurement and optimization of the polarization-dependent modulation depth in super-resolution structured illumination microscopy |
| title_short | Direct measurement and optimization of the polarization-dependent modulation depth in super-resolution structured illumination microscopy |
| title_sort | direct measurement and optimization of the polarization dependent modulation depth in super resolution structured illumination microscopy |
| topic | Structured illumination microscopy depolarization modulation depth phase compensation |
| url | https://www.worldscientific.com/doi/10.1142/S1793545825500051 |
| work_keys_str_mv | AT linbowang directmeasurementandoptimizationofthepolarizationdependentmodulationdepthinsuperresolutionstructuredilluminationmicroscopy AT siminli directmeasurementandoptimizationofthepolarizationdependentmodulationdepthinsuperresolutionstructuredilluminationmicroscopy AT xiaohuchen directmeasurementandoptimizationofthepolarizationdependentmodulationdepthinsuperresolutionstructuredilluminationmicroscopy AT xinjin directmeasurementandoptimizationofthepolarizationdependentmodulationdepthinsuperresolutionstructuredilluminationmicroscopy AT jiezhang directmeasurementandoptimizationofthepolarizationdependentmodulationdepthinsuperresolutionstructuredilluminationmicroscopy AT huili directmeasurementandoptimizationofthepolarizationdependentmodulationdepthinsuperresolutionstructuredilluminationmicroscopy AT gangwen directmeasurementandoptimizationofthepolarizationdependentmodulationdepthinsuperresolutionstructuredilluminationmicroscopy |