Effects on antioxidant responses, stress indicator and immune responses of olive flounder, Paralichthys olivaceus raised in bio-floc culture or seawater culture challenged by Streptococcus iniae

Olive flounders, Paralichthys olivaceus (mean weight 1,027 ± 65 g, mean length 44.03 ± 3.1 cm) were raised in bio-floc culture or seawater culture for 10 months. The P. olivaceus raised in bio-floc culture or seawater culture were inoculated at each S. iniae concentrations at 0, 3.36 × 106, 3.36 × 1...

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Main Authors: Saebom Sohn, A-Hyun Jo, Cheol Young Choi, Ju-Chan Kang, Jun-Hwan Kim
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Comparative Immunology Reports
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Online Access:http://www.sciencedirect.com/science/article/pii/S2950311625000217
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author Saebom Sohn
A-Hyun Jo
Cheol Young Choi
Ju-Chan Kang
Jun-Hwan Kim
author_facet Saebom Sohn
A-Hyun Jo
Cheol Young Choi
Ju-Chan Kang
Jun-Hwan Kim
author_sort Saebom Sohn
collection DOAJ
description Olive flounders, Paralichthys olivaceus (mean weight 1,027 ± 65 g, mean length 44.03 ± 3.1 cm) were raised in bio-floc culture or seawater culture for 10 months. The P. olivaceus raised in bio-floc culture or seawater culture were inoculated at each S. iniae concentrations at 0, 3.36 × 106, 3.36 × 107, 3.36 × 108, and 3.36 × 109 CFU/g fish for 96 h. In antioxidant responses, SOD, CAT and GST activities were significantly increased, whereas GSH level was significantly decreased, with more pronounced changes observed in the seawater culture group. In stress indicators, HSP 70 and cortisol levels were significantly increased in both groups. In immune responses, lysozyme activity and Ig M levels were significantly decreased, with greater reductions in the seawater culture group compared to the bio-floc culture group. These results indicate that S. iniae infection has significant negative effects on antioxidant responses, stress indicators, and immune responses in P. olivaceus, and that these effects are more severe in fish raised in seawater culture than those in bio-floc culture.
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issn 2950-3116
language English
publishDate 2025-06-01
publisher Elsevier
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series Comparative Immunology Reports
spelling doaj-art-688fea3758e541d8afd90f43d352149f2025-08-20T03:45:44ZengElsevierComparative Immunology Reports2950-31162025-06-01820021510.1016/j.cirep.2025.200215Effects on antioxidant responses, stress indicator and immune responses of olive flounder, Paralichthys olivaceus raised in bio-floc culture or seawater culture challenged by Streptococcus iniaeSaebom Sohn0A-Hyun Jo1Cheol Young Choi2Ju-Chan Kang3Jun-Hwan Kim4General Service Division, Regional Office of National Fishery Products Quality Management Service, Busan 49111, South KoreaDepartment of Marine Life Science, Jeju National University, Jeju 63243, Republic of KoreaDivision of Marine BioScience, National Korea Maritime and Ocean University, Busan 49112, Republic of Korea; Corresponding authors.Department of Aquatic Life Medicine, Pukyong National University, Busan, South Korea; Corresponding authors.Department of Marine Life Science, Jeju National University, Jeju 63243, Republic of Korea; Department of Aquatic Life Medicine, College of Ocean Sciences, Jeju National University, Jeju 63243, South Korea; Corresponding authors.Olive flounders, Paralichthys olivaceus (mean weight 1,027 ± 65 g, mean length 44.03 ± 3.1 cm) were raised in bio-floc culture or seawater culture for 10 months. The P. olivaceus raised in bio-floc culture or seawater culture were inoculated at each S. iniae concentrations at 0, 3.36 × 106, 3.36 × 107, 3.36 × 108, and 3.36 × 109 CFU/g fish for 96 h. In antioxidant responses, SOD, CAT and GST activities were significantly increased, whereas GSH level was significantly decreased, with more pronounced changes observed in the seawater culture group. In stress indicators, HSP 70 and cortisol levels were significantly increased in both groups. In immune responses, lysozyme activity and Ig M levels were significantly decreased, with greater reductions in the seawater culture group compared to the bio-floc culture group. These results indicate that S. iniae infection has significant negative effects on antioxidant responses, stress indicators, and immune responses in P. olivaceus, and that these effects are more severe in fish raised in seawater culture than those in bio-floc culture.http://www.sciencedirect.com/science/article/pii/S2950311625000217Paralichthys olivaceusBio-floc cultureStreptococcus iniae challengeAntioxidant and immune responsesStress indicators
spellingShingle Saebom Sohn
A-Hyun Jo
Cheol Young Choi
Ju-Chan Kang
Jun-Hwan Kim
Effects on antioxidant responses, stress indicator and immune responses of olive flounder, Paralichthys olivaceus raised in bio-floc culture or seawater culture challenged by Streptococcus iniae
Comparative Immunology Reports
Paralichthys olivaceus
Bio-floc culture
Streptococcus iniae challenge
Antioxidant and immune responses
Stress indicators
title Effects on antioxidant responses, stress indicator and immune responses of olive flounder, Paralichthys olivaceus raised in bio-floc culture or seawater culture challenged by Streptococcus iniae
title_full Effects on antioxidant responses, stress indicator and immune responses of olive flounder, Paralichthys olivaceus raised in bio-floc culture or seawater culture challenged by Streptococcus iniae
title_fullStr Effects on antioxidant responses, stress indicator and immune responses of olive flounder, Paralichthys olivaceus raised in bio-floc culture or seawater culture challenged by Streptococcus iniae
title_full_unstemmed Effects on antioxidant responses, stress indicator and immune responses of olive flounder, Paralichthys olivaceus raised in bio-floc culture or seawater culture challenged by Streptococcus iniae
title_short Effects on antioxidant responses, stress indicator and immune responses of olive flounder, Paralichthys olivaceus raised in bio-floc culture or seawater culture challenged by Streptococcus iniae
title_sort effects on antioxidant responses stress indicator and immune responses of olive flounder paralichthys olivaceus raised in bio floc culture or seawater culture challenged by streptococcus iniae
topic Paralichthys olivaceus
Bio-floc culture
Streptococcus iniae challenge
Antioxidant and immune responses
Stress indicators
url http://www.sciencedirect.com/science/article/pii/S2950311625000217
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