Synchrotron-based X-ray 3D phase contrast imaging and analysis of transmural myocardial tissue from heart failure patients
Abstract Synchrotron-based X-ray phase contrast imaging (X-PCI) is a non-destructive imaging modality that can provide high resolution three-dimensional (3D) visualisation of transmural myocardial tissue, collagen matrix reconstruction, and quantification of myocyte aggregate orientation (‘myomappin...
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Nature Portfolio
2025-07-01
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| Online Access: | https://doi.org/10.1038/s41598-025-04012-5 |
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| author | Nikola Skreb Filip Loncaric Kan Yan Chloe Li Anne Bonnin Hector Dejea Patricia Garcia-Canadilla Ivana Ilic Hrvoje Gasparovic Davor Milicic Bart Bijnens Andrew C. Cook Ivo Planinc Maja Cikes |
| author_facet | Nikola Skreb Filip Loncaric Kan Yan Chloe Li Anne Bonnin Hector Dejea Patricia Garcia-Canadilla Ivana Ilic Hrvoje Gasparovic Davor Milicic Bart Bijnens Andrew C. Cook Ivo Planinc Maja Cikes |
| author_sort | Nikola Skreb |
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| description | Abstract Synchrotron-based X-ray phase contrast imaging (X-PCI) is a non-destructive imaging modality that can provide high resolution three-dimensional (3D) visualisation of transmural myocardial tissue, collagen matrix reconstruction, and quantification of myocyte aggregate orientation (‘myomapping’). We aimed to use X-PCI to analyse microstructural features in transmural myocardial samples from patients with advanced heart failure. Six patients were included: two receiving a left ventricular assist device (LVAD) for ischaemic (ICM) and dilated cardiomyopathy (DCM), and four undergoing heart transplantation (HTx), two for the ICM, one for DCM and one for toxic cardiomyopathy. Samples were obtained by left ventricular (LV) apical coring (LVAD group) or from the LV free wall of the explanted hearts (HTx group) and imaged by X-PCI using a multi-scale setup (maximal resolution at 0.65 µm pixel size). The 3D image datasets were analysed via two-dimensional orthogonal cuts in different layers. Visualisation and quantification of the myocyte aggregates orientation showed a disruption in epicardial-to-endocardial transition in DCM, whereas the collagen matrix reconstruction identified characteristic fibrosis patterns amongst different HF aetiologies. In conclusion, X-PCI is a 3D imaging method that can extend the amount of information available from ex-vivo tissue analysis and, as an addition to multimodal imaging protocols, potentially improve disease phenotyping. |
| format | Article |
| id | doaj-art-688a0d56107b419a8ec4e6dd3a37817d |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-688a0d56107b419a8ec4e6dd3a37817d2025-08-20T04:02:56ZengNature PortfolioScientific Reports2045-23222025-07-0115111110.1038/s41598-025-04012-5Synchrotron-based X-ray 3D phase contrast imaging and analysis of transmural myocardial tissue from heart failure patientsNikola Skreb0Filip Loncaric1Kan Yan Chloe Li2Anne Bonnin3Hector Dejea4Patricia Garcia-Canadilla5Ivana Ilic6Hrvoje Gasparovic7Davor Milicic8Bart Bijnens9Andrew C. Cook10Ivo Planinc11Maja Cikes12Department of Cardiovascular Diseases, University of Zagreb School of Medicine, University Hospital Centre ZagrebDepartment of Cardiovascular Diseases, University of Zagreb School of Medicine, University Hospital Centre ZagrebInstitute of Cardiovascular Science, University College LondonSwiss Light Source, Paul Scherrer InstituteEuropean Synchrotron Radiation FacilityCardiovascular Diseases and Child Development, Sant Joan de Déu Research Institute (IRSJD)Department of Pathology and Cytology, University of Zagreb School of Medicine, University Hospital Centre ZagrebDepartment of Cardiac Surgery, University of Zagreb School of Medicine, University Hospital Centre ZagrebDepartment of Cardiovascular Diseases, University of Zagreb School of Medicine, University Hospital Centre ZagrebCatalan Institution for Research and Advanced Studies, ICREAInstitute of Cardiovascular Science, University College LondonDepartment of Cardiovascular Diseases, University of Zagreb School of Medicine, University Hospital Centre ZagrebDepartment of Cardiovascular Diseases, University of Zagreb School of Medicine, University Hospital Centre ZagrebAbstract Synchrotron-based X-ray phase contrast imaging (X-PCI) is a non-destructive imaging modality that can provide high resolution three-dimensional (3D) visualisation of transmural myocardial tissue, collagen matrix reconstruction, and quantification of myocyte aggregate orientation (‘myomapping’). We aimed to use X-PCI to analyse microstructural features in transmural myocardial samples from patients with advanced heart failure. Six patients were included: two receiving a left ventricular assist device (LVAD) for ischaemic (ICM) and dilated cardiomyopathy (DCM), and four undergoing heart transplantation (HTx), two for the ICM, one for DCM and one for toxic cardiomyopathy. Samples were obtained by left ventricular (LV) apical coring (LVAD group) or from the LV free wall of the explanted hearts (HTx group) and imaged by X-PCI using a multi-scale setup (maximal resolution at 0.65 µm pixel size). The 3D image datasets were analysed via two-dimensional orthogonal cuts in different layers. Visualisation and quantification of the myocyte aggregates orientation showed a disruption in epicardial-to-endocardial transition in DCM, whereas the collagen matrix reconstruction identified characteristic fibrosis patterns amongst different HF aetiologies. In conclusion, X-PCI is a 3D imaging method that can extend the amount of information available from ex-vivo tissue analysis and, as an addition to multimodal imaging protocols, potentially improve disease phenotyping.https://doi.org/10.1038/s41598-025-04012-5Heart failureCardiomyopathyHeart transplantationLeft ventricular assist deviceX-ray phase contrast imagingHistopathology |
| spellingShingle | Nikola Skreb Filip Loncaric Kan Yan Chloe Li Anne Bonnin Hector Dejea Patricia Garcia-Canadilla Ivana Ilic Hrvoje Gasparovic Davor Milicic Bart Bijnens Andrew C. Cook Ivo Planinc Maja Cikes Synchrotron-based X-ray 3D phase contrast imaging and analysis of transmural myocardial tissue from heart failure patients Scientific Reports Heart failure Cardiomyopathy Heart transplantation Left ventricular assist device X-ray phase contrast imaging Histopathology |
| title | Synchrotron-based X-ray 3D phase contrast imaging and analysis of transmural myocardial tissue from heart failure patients |
| title_full | Synchrotron-based X-ray 3D phase contrast imaging and analysis of transmural myocardial tissue from heart failure patients |
| title_fullStr | Synchrotron-based X-ray 3D phase contrast imaging and analysis of transmural myocardial tissue from heart failure patients |
| title_full_unstemmed | Synchrotron-based X-ray 3D phase contrast imaging and analysis of transmural myocardial tissue from heart failure patients |
| title_short | Synchrotron-based X-ray 3D phase contrast imaging and analysis of transmural myocardial tissue from heart failure patients |
| title_sort | synchrotron based x ray 3d phase contrast imaging and analysis of transmural myocardial tissue from heart failure patients |
| topic | Heart failure Cardiomyopathy Heart transplantation Left ventricular assist device X-ray phase contrast imaging Histopathology |
| url | https://doi.org/10.1038/s41598-025-04012-5 |
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