A Mediterranean-like fat blend protects against the development of severe colitis in the mucin-2 deficient murine model
There is a growing appreciation that the interaction between diet, the gut microbiota and the immune system contribute to the development and progression of inflammatory bowel disease (IBD). A mounting body of scientific evidence suggests that high-fat diets exacerbate IBD; however, there is a lack...
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Taylor & Francis Group
2022-12-01
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| Series: | Gut Microbes |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19490976.2022.2055441 |
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| author | Natasha Haskey Jiayu Ye Mehrbod Estaki Andrea A. Verdugo Meza Jacqueline A. Barnett Mitra Yousefi Blake W. Birnie Samantha Gruenheid Sanjoy Ghosh Deanna L. Gibson |
| author_facet | Natasha Haskey Jiayu Ye Mehrbod Estaki Andrea A. Verdugo Meza Jacqueline A. Barnett Mitra Yousefi Blake W. Birnie Samantha Gruenheid Sanjoy Ghosh Deanna L. Gibson |
| author_sort | Natasha Haskey |
| collection | DOAJ |
| description | There is a growing appreciation that the interaction between diet, the gut microbiota and the immune system contribute to the development and progression of inflammatory bowel disease (IBD). A mounting body of scientific evidence suggests that high-fat diets exacerbate IBD; however, there is a lack of information on how specific types of fat impact colitis. The Mediterranean diet (MD) is considered a health-promoting diet containing approximately 40% total fat. It is not known if the blend of fats found in the MD contributes to its beneficial protective effects. Mice deficient in the mucin 2 gene (Muc 2−/−) were weaned to 40% fat, isocaloric, isonitrogenous diets. We compared the MD fat blend (high monounsaturated, 2:1 n-6:n-3 polyunsaturated and moderate saturated fat) to diets composed of corn oil (CO, n-6 polyunsaturated-rich), olive oil (monounsaturated-rich) or milk fat (MF, saturated-rich) on spontaneous colitis development in Muc2−/− mice. The MD resulted in lower clinical and histopathological scores and induced tolerogenic CD103+ CD11b+ dendritic, Th22 and IL-17+ IL-22+ cells necessary for intestinal barrier repair. The MD was associated with beneficial microbes and associated with higher cecal acetic acid levels negatively correlated with colitogenic microbes like Akkermansia muciniphila. In contrast, CO showed a higher prevalence of mucin-degraders including A. muciniphila and Enterobacteriaceae, which have been associated with colitis. A dietary blend of fats mimicking the MD, reduces disease activity, inflammation-related biomarkers and improves metabolic parameters in the Muc2−/− mouse model. Our findings suggest that the MD fat blend could be incorporated into a maintenance diet for colitis. |
| format | Article |
| id | doaj-art-687f641f1a3f4e3ca780f12240888cf3 |
| institution | DOAJ |
| issn | 1949-0976 1949-0984 |
| language | English |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gut Microbes |
| spelling | doaj-art-687f641f1a3f4e3ca780f12240888cf32025-08-20T03:12:50ZengTaylor & Francis GroupGut Microbes1949-09761949-09842022-12-0114110.1080/19490976.2022.2055441A Mediterranean-like fat blend protects against the development of severe colitis in the mucin-2 deficient murine modelNatasha Haskey0Jiayu Ye1Mehrbod Estaki2Andrea A. Verdugo Meza3Jacqueline A. Barnett4Mitra Yousefi5Blake W. Birnie6Samantha Gruenheid7Sanjoy Ghosh8Deanna L. Gibson9Department of Biology, University of British Columbia - Okanagan Campus; Kelowna, British Columbia, CanadaDepartment of Biology, University of British Columbia - Okanagan Campus; Kelowna, British Columbia, CanadaDepartment of Pediatrics, University of California, San Diego, La Jolla, California, USADepartment of Biology, University of British Columbia - Okanagan Campus; Kelowna, British Columbia, CanadaDepartment of Biology, University of British Columbia - Okanagan Campus; Kelowna, British Columbia, CanadaThe Center for Phenogenomics Infection & McGill University Research Centre on Complex Traits; McGill University, Montreal, Quebec, CanadaDepartment of Medicine, University of British Columbia - Okanagan Campus, Kelowna, British Columbia, CanadaAssociate Professor - Department of Microbiology and Immunology, McGill University, Montreal, Quebec, CanadaDepartment of Biology, University of British Columbia - Okanagan Campus; Kelowna, British Columbia, CanadaDepartment of Biology, University of British Columbia - Okanagan Campus; Kelowna, British Columbia, CanadaThere is a growing appreciation that the interaction between diet, the gut microbiota and the immune system contribute to the development and progression of inflammatory bowel disease (IBD). A mounting body of scientific evidence suggests that high-fat diets exacerbate IBD; however, there is a lack of information on how specific types of fat impact colitis. The Mediterranean diet (MD) is considered a health-promoting diet containing approximately 40% total fat. It is not known if the blend of fats found in the MD contributes to its beneficial protective effects. Mice deficient in the mucin 2 gene (Muc 2−/−) were weaned to 40% fat, isocaloric, isonitrogenous diets. We compared the MD fat blend (high monounsaturated, 2:1 n-6:n-3 polyunsaturated and moderate saturated fat) to diets composed of corn oil (CO, n-6 polyunsaturated-rich), olive oil (monounsaturated-rich) or milk fat (MF, saturated-rich) on spontaneous colitis development in Muc2−/− mice. The MD resulted in lower clinical and histopathological scores and induced tolerogenic CD103+ CD11b+ dendritic, Th22 and IL-17+ IL-22+ cells necessary for intestinal barrier repair. The MD was associated with beneficial microbes and associated with higher cecal acetic acid levels negatively correlated with colitogenic microbes like Akkermansia muciniphila. In contrast, CO showed a higher prevalence of mucin-degraders including A. muciniphila and Enterobacteriaceae, which have been associated with colitis. A dietary blend of fats mimicking the MD, reduces disease activity, inflammation-related biomarkers and improves metabolic parameters in the Muc2−/− mouse model. Our findings suggest that the MD fat blend could be incorporated into a maintenance diet for colitis.https://www.tandfonline.com/doi/10.1080/19490976.2022.2055441Colitisulcerative colitisinflammationdietary fatmediterranean dietmucin 2 |
| spellingShingle | Natasha Haskey Jiayu Ye Mehrbod Estaki Andrea A. Verdugo Meza Jacqueline A. Barnett Mitra Yousefi Blake W. Birnie Samantha Gruenheid Sanjoy Ghosh Deanna L. Gibson A Mediterranean-like fat blend protects against the development of severe colitis in the mucin-2 deficient murine model Gut Microbes Colitis ulcerative colitis inflammation dietary fat mediterranean diet mucin 2 |
| title | A Mediterranean-like fat blend protects against the development of severe colitis in the mucin-2 deficient murine model |
| title_full | A Mediterranean-like fat blend protects against the development of severe colitis in the mucin-2 deficient murine model |
| title_fullStr | A Mediterranean-like fat blend protects against the development of severe colitis in the mucin-2 deficient murine model |
| title_full_unstemmed | A Mediterranean-like fat blend protects against the development of severe colitis in the mucin-2 deficient murine model |
| title_short | A Mediterranean-like fat blend protects against the development of severe colitis in the mucin-2 deficient murine model |
| title_sort | mediterranean like fat blend protects against the development of severe colitis in the mucin 2 deficient murine model |
| topic | Colitis ulcerative colitis inflammation dietary fat mediterranean diet mucin 2 |
| url | https://www.tandfonline.com/doi/10.1080/19490976.2022.2055441 |
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