Organ‐on‐chip for advancing CAR therapy

Abstract Despite great strides of progress, at least 60% of the responding patients relapse to CAR therapy across the blood malignancies. Off‐tumor toxicity apart from functional deficits, cytopenia and infection are the major unfavourable effect of CAR therapy. Models, which faithfully recapitulate...

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Main Authors: Lightson Ngashangva, Sunil Martin
Format: Article
Language:English
Published: Wiley 2025-01-01
Series:Clinical & Translational Immunology
Subjects:
Online Access:https://doi.org/10.1002/cti2.70024
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author Lightson Ngashangva
Sunil Martin
author_facet Lightson Ngashangva
Sunil Martin
author_sort Lightson Ngashangva
collection DOAJ
description Abstract Despite great strides of progress, at least 60% of the responding patients relapse to CAR therapy across the blood malignancies. Off‐tumor toxicity apart from functional deficits, cytopenia and infection are the major unfavourable effect of CAR therapy. Models, which faithfully recapitulate the physiology and complexities of immunocompetent tumor microenvironment (TME), paused challenges in capturing potential off‐tumor effects of CAR therapy. Importantly, a landmark change in the legislation allows US Food and Drug Administration and New Drugs and Clinical Trial Rules in India encourages researchers to replace animal testing with cell culture approaches relevant to human system. Organ‐on‐chip (OOC) based on microfluidics technology can potentially emulate multiple biochemical and biophysical intricacies of blood and lymph flow at microscale. Nonetheless, how the evolving microfluidics technology can be enabling to real‐time testing of cell and gene is yet to be realised.
format Article
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institution DOAJ
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publisher Wiley
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series Clinical & Translational Immunology
spelling doaj-art-686ce29243d84d3ebda4ad0dab6611d62025-08-20T02:45:33ZengWileyClinical & Translational Immunology2050-00682025-01-01142n/an/a10.1002/cti2.70024Organ‐on‐chip for advancing CAR therapyLightson Ngashangva0Sunil Martin1Biosensors and Biomicrofluidics Laboratory, Transdisciplinary Biology Program Rajiv Gandhi Centre for Biotechnology (RGCB) Thiruvananthapuram Kerala IndiaSynthetic Immunology Laboratory, Division of Cancer Research Rajiv Gandhi Centre for Biotechnology (RGCB) Thiruvananthapuram Kerala IndiaAbstract Despite great strides of progress, at least 60% of the responding patients relapse to CAR therapy across the blood malignancies. Off‐tumor toxicity apart from functional deficits, cytopenia and infection are the major unfavourable effect of CAR therapy. Models, which faithfully recapitulate the physiology and complexities of immunocompetent tumor microenvironment (TME), paused challenges in capturing potential off‐tumor effects of CAR therapy. Importantly, a landmark change in the legislation allows US Food and Drug Administration and New Drugs and Clinical Trial Rules in India encourages researchers to replace animal testing with cell culture approaches relevant to human system. Organ‐on‐chip (OOC) based on microfluidics technology can potentially emulate multiple biochemical and biophysical intricacies of blood and lymph flow at microscale. Nonetheless, how the evolving microfluidics technology can be enabling to real‐time testing of cell and gene is yet to be realised.https://doi.org/10.1002/cti2.70024CAR therapymicrofluidicsorgan‐on‐chiptumor immune microenvironment
spellingShingle Lightson Ngashangva
Sunil Martin
Organ‐on‐chip for advancing CAR therapy
Clinical & Translational Immunology
CAR therapy
microfluidics
organ‐on‐chip
tumor immune microenvironment
title Organ‐on‐chip for advancing CAR therapy
title_full Organ‐on‐chip for advancing CAR therapy
title_fullStr Organ‐on‐chip for advancing CAR therapy
title_full_unstemmed Organ‐on‐chip for advancing CAR therapy
title_short Organ‐on‐chip for advancing CAR therapy
title_sort organ on chip for advancing car therapy
topic CAR therapy
microfluidics
organ‐on‐chip
tumor immune microenvironment
url https://doi.org/10.1002/cti2.70024
work_keys_str_mv AT lightsonngashangva organonchipforadvancingcartherapy
AT sunilmartin organonchipforadvancingcartherapy