miR-134-3p Regulates Cell Proliferation and Apoptosis by Targeting INHBA via Inhibiting the TGF-β/PI3K/AKT Pathway in Sheep Granulosa Cells
Inhibin β-A (<i>INHBA</i>), a TGF-β superfamily member, is crucial for developing follicles. Although miRNAs are essential for post-transcriptional gene regulation, it is not yet known how they affect the expression of <i>INHBA</i> during follicle development. Using bioinform...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-7737/14/1/24 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832589048249581568 |
|---|---|
| author | Xinai Huang Yongjin Bao Fan Yang Xiaodan Li Feng Wang Chunxiang Zhang |
| author_facet | Xinai Huang Yongjin Bao Fan Yang Xiaodan Li Feng Wang Chunxiang Zhang |
| author_sort | Xinai Huang |
| collection | DOAJ |
| description | Inhibin β-A (<i>INHBA</i>), a TGF-β superfamily member, is crucial for developing follicles. Although miRNAs are essential for post-transcriptional gene regulation, it is not yet known how they affect the expression of <i>INHBA</i> during follicle development. Using bioinformatics analyses, miR-134-3p was found, in this investigation, to be a crucial microRNA that targets <i>INHBA</i> in sheep GCs. Furthermore, when the follicular diameter expanded, there was a discernible decline in miR-134-3p expression. The miR-134-3p overexpression markedly reduced the proliferation of GCs, whereas its knockdown augmented it. Moreover, cell cycle progression was enhanced by miR-134-3p overexpression. Furthermore, miR-134-3p overexpression heightened GC apoptosis, while its knockdown reduced it. Importantly, miR-134-3p overexpression blocked the PI3K/AKT/mTOR axis, whereas its knockdown stimulated it. Overall, the outcomes of transfections with <i>INHBA</i> and miR-134-3p showed that, in sheep GCs, miR-134-3p targets <i>INHBA</i> to control cell proliferation and apoptosis. In summary, these results add to our understanding of the molecular mechanisms involving important miRNAs in ewe fecundity by indicating that miR-134-3p influences cell proliferation, cell apoptosis, and the TGF-β/PI3K/AKT/mTOR axis, which, in turn, influences the follicular development of sheep GCs. |
| format | Article |
| id | doaj-art-686c868094204787a142f1ccb9370f5e |
| institution | Kabale University |
| issn | 2079-7737 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj-art-686c868094204787a142f1ccb9370f5e2025-01-24T13:23:20ZengMDPI AGBiology2079-77372024-12-011412410.3390/biology14010024miR-134-3p Regulates Cell Proliferation and Apoptosis by Targeting INHBA via Inhibiting the TGF-β/PI3K/AKT Pathway in Sheep Granulosa CellsXinai Huang0Yongjin Bao1Fan Yang2Xiaodan Li3Feng Wang4Chunxiang Zhang5College of Animal Science, Shanxi Agricultural University, Taigu 030801, ChinaJiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing 210095, ChinaJiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing 210095, ChinaJiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing 210095, ChinaJiangsu Livestock Embryo Engineering Laboratory, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Animal Science, Shanxi Agricultural University, Taigu 030801, ChinaInhibin β-A (<i>INHBA</i>), a TGF-β superfamily member, is crucial for developing follicles. Although miRNAs are essential for post-transcriptional gene regulation, it is not yet known how they affect the expression of <i>INHBA</i> during follicle development. Using bioinformatics analyses, miR-134-3p was found, in this investigation, to be a crucial microRNA that targets <i>INHBA</i> in sheep GCs. Furthermore, when the follicular diameter expanded, there was a discernible decline in miR-134-3p expression. The miR-134-3p overexpression markedly reduced the proliferation of GCs, whereas its knockdown augmented it. Moreover, cell cycle progression was enhanced by miR-134-3p overexpression. Furthermore, miR-134-3p overexpression heightened GC apoptosis, while its knockdown reduced it. Importantly, miR-134-3p overexpression blocked the PI3K/AKT/mTOR axis, whereas its knockdown stimulated it. Overall, the outcomes of transfections with <i>INHBA</i> and miR-134-3p showed that, in sheep GCs, miR-134-3p targets <i>INHBA</i> to control cell proliferation and apoptosis. In summary, these results add to our understanding of the molecular mechanisms involving important miRNAs in ewe fecundity by indicating that miR-134-3p influences cell proliferation, cell apoptosis, and the TGF-β/PI3K/AKT/mTOR axis, which, in turn, influences the follicular development of sheep GCs.https://www.mdpi.com/2079-7737/14/1/24miR-134-3pINHBAproliferationapoptosissheep granulosa cells |
| spellingShingle | Xinai Huang Yongjin Bao Fan Yang Xiaodan Li Feng Wang Chunxiang Zhang miR-134-3p Regulates Cell Proliferation and Apoptosis by Targeting INHBA via Inhibiting the TGF-β/PI3K/AKT Pathway in Sheep Granulosa Cells Biology miR-134-3p INHBA proliferation apoptosis sheep granulosa cells |
| title | miR-134-3p Regulates Cell Proliferation and Apoptosis by Targeting INHBA via Inhibiting the TGF-β/PI3K/AKT Pathway in Sheep Granulosa Cells |
| title_full | miR-134-3p Regulates Cell Proliferation and Apoptosis by Targeting INHBA via Inhibiting the TGF-β/PI3K/AKT Pathway in Sheep Granulosa Cells |
| title_fullStr | miR-134-3p Regulates Cell Proliferation and Apoptosis by Targeting INHBA via Inhibiting the TGF-β/PI3K/AKT Pathway in Sheep Granulosa Cells |
| title_full_unstemmed | miR-134-3p Regulates Cell Proliferation and Apoptosis by Targeting INHBA via Inhibiting the TGF-β/PI3K/AKT Pathway in Sheep Granulosa Cells |
| title_short | miR-134-3p Regulates Cell Proliferation and Apoptosis by Targeting INHBA via Inhibiting the TGF-β/PI3K/AKT Pathway in Sheep Granulosa Cells |
| title_sort | mir 134 3p regulates cell proliferation and apoptosis by targeting inhba via inhibiting the tgf β pi3k akt pathway in sheep granulosa cells |
| topic | miR-134-3p INHBA proliferation apoptosis sheep granulosa cells |
| url | https://www.mdpi.com/2079-7737/14/1/24 |
| work_keys_str_mv | AT xinaihuang mir1343pregulatescellproliferationandapoptosisbytargetinginhbaviainhibitingthetgfbpi3kaktpathwayinsheepgranulosacells AT yongjinbao mir1343pregulatescellproliferationandapoptosisbytargetinginhbaviainhibitingthetgfbpi3kaktpathwayinsheepgranulosacells AT fanyang mir1343pregulatescellproliferationandapoptosisbytargetinginhbaviainhibitingthetgfbpi3kaktpathwayinsheepgranulosacells AT xiaodanli mir1343pregulatescellproliferationandapoptosisbytargetinginhbaviainhibitingthetgfbpi3kaktpathwayinsheepgranulosacells AT fengwang mir1343pregulatescellproliferationandapoptosisbytargetinginhbaviainhibitingthetgfbpi3kaktpathwayinsheepgranulosacells AT chunxiangzhang mir1343pregulatescellproliferationandapoptosisbytargetinginhbaviainhibitingthetgfbpi3kaktpathwayinsheepgranulosacells |