Pathogenic Menace of MDR Bacteria in Stool and Throat Swab Surveillance Cultures of Hematopoietic Stem Cell Recipients and Evaluation of Post-Transplant Bloodstream Infections: A Cross-Sectional Study from A Tertiary Care Teaching Hospital of Kolkata, India
Background Infections with multidrug-resistant organisms (MDRO) may pose a threat to hematopoietic stem cell transplant recipients. Pre-transplant surveillance culture of stool and throat swabs may guide antibiotic therapy in these groups of patients. We have conducted this study to determine the...
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Medsci Publications
2025-01-01
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| Series: | National Journal of Medical Research |
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| Online Access: | https://njmr.in/index.php/file/article/view/1035 |
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| author | Kabita Choudhury Sonia Deb Suman Kundu Baishali Dhar Tuphan Kanti Dolai Jayanta Bikash Dey Swagata Ganguly Bhattacharjee |
| author_facet | Kabita Choudhury Sonia Deb Suman Kundu Baishali Dhar Tuphan Kanti Dolai Jayanta Bikash Dey Swagata Ganguly Bhattacharjee |
| author_sort | Kabita Choudhury |
| collection | DOAJ |
| description | Background
Infections with multidrug-resistant organisms (MDRO) may pose a threat to hematopoietic stem cell transplant recipients. Pre-transplant surveillance culture of stool and throat swabs may guide antibiotic therapy in these groups of patients. We have conducted this study to determine the profile of organisms isolated from stool and throat swab cultures, their susceptibility pattern, and the presence of MDRO to detect post-transplant bloodstream infections in this tertiary care teaching hospital in Kolkata.
Methods
This cross-sectional study was conducted over a period of one year between 1st January to 31st December 2023. Pre-transplant surveillance culture of stool and throat swabs were performed on seventy patients from the Department of Hematology and processed in the Department of Microbiology using standard laboratory guidelines and their antibiotic susceptibility was done as per CLSI 2023 guidelines. The organisms were phenotypically screened for drug resistance and molecular confirmation was done for carbapenemases producers. Bloodstream infections in these recipients were detected by blood culture.
Results
There were 70 transplant recipients in whom stool surveillance culture yielded maximum incidence of Escherichia coli of which 32.8% were MDRO. Throat surveillance culture yielded Escherichia coli with 51.8% MDRO. blaNDM was the most common carbapenemases gene detected. Post-transplant the incidence of bloodstream infections detected by blood culture was seen in 32 recipients with Acinetobacter baumannii being the predominant Gram-negative isolate.
Conclusion
Colonization with MDRO in HSCT recipients before transplant is a potential threat. Performing stool and throat surveillance cultures before transplantation can inform empirical antibiotic strategies and tailored individualized antibiotic treatment.
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| format | Article |
| id | doaj-art-685bcb9af6474234a160a140a2ff62ea |
| institution | DOAJ |
| issn | 2249-4995 2277-8810 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Medsci Publications |
| record_format | Article |
| series | National Journal of Medical Research |
| spelling | doaj-art-685bcb9af6474234a160a140a2ff62ea2025-08-20T02:44:03ZengMedsci PublicationsNational Journal of Medical Research2249-49952277-88102025-01-01150110.55489/njmr.150120251035Pathogenic Menace of MDR Bacteria in Stool and Throat Swab Surveillance Cultures of Hematopoietic Stem Cell Recipients and Evaluation of Post-Transplant Bloodstream Infections: A Cross-Sectional Study from A Tertiary Care Teaching Hospital of Kolkata, IndiaKabita Choudhury0https://orcid.org/0000-0002-4438-4208Sonia Deb1https://orcid.org/0009-0002-2924-5442Suman Kundu2https://orcid.org/0000-0001-8861-7061Baishali Dhar3Tuphan Kanti Dolai4https://orcid.org/0000-0002-4688-584XJayanta Bikash Dey5Swagata Ganguly Bhattacharjee6https://orcid.org/0000-0002-5978-778XDepartment of Microbiology, Burdwan Medical College, Burdwan, IndiaDepartment of Microbiology, NRS Medical College, Kolkata, IndiaDepartment of Microbiology, NRS Medical College, Kolkata, IndiaDepartment of Microbiology, NRS Medical College, Kolkata, IndiaDepartment of Hematology, NRS Medical College, Kolkata, IndiaDepartment of Microbiology, NRS Medical College, Kolkata, IndiaDepartment of Microbiology, NRS Medical College, Kolkata, IndiaBackground Infections with multidrug-resistant organisms (MDRO) may pose a threat to hematopoietic stem cell transplant recipients. Pre-transplant surveillance culture of stool and throat swabs may guide antibiotic therapy in these groups of patients. We have conducted this study to determine the profile of organisms isolated from stool and throat swab cultures, their susceptibility pattern, and the presence of MDRO to detect post-transplant bloodstream infections in this tertiary care teaching hospital in Kolkata. Methods This cross-sectional study was conducted over a period of one year between 1st January to 31st December 2023. Pre-transplant surveillance culture of stool and throat swabs were performed on seventy patients from the Department of Hematology and processed in the Department of Microbiology using standard laboratory guidelines and their antibiotic susceptibility was done as per CLSI 2023 guidelines. The organisms were phenotypically screened for drug resistance and molecular confirmation was done for carbapenemases producers. Bloodstream infections in these recipients were detected by blood culture. Results There were 70 transplant recipients in whom stool surveillance culture yielded maximum incidence of Escherichia coli of which 32.8% were MDRO. Throat surveillance culture yielded Escherichia coli with 51.8% MDRO. blaNDM was the most common carbapenemases gene detected. Post-transplant the incidence of bloodstream infections detected by blood culture was seen in 32 recipients with Acinetobacter baumannii being the predominant Gram-negative isolate. Conclusion Colonization with MDRO in HSCT recipients before transplant is a potential threat. Performing stool and throat surveillance cultures before transplantation can inform empirical antibiotic strategies and tailored individualized antibiotic treatment. https://njmr.in/index.php/file/article/view/1035Bone marrow cell transplantationStool surveillance cultureDrug ResistanceBacteremia |
| spellingShingle | Kabita Choudhury Sonia Deb Suman Kundu Baishali Dhar Tuphan Kanti Dolai Jayanta Bikash Dey Swagata Ganguly Bhattacharjee Pathogenic Menace of MDR Bacteria in Stool and Throat Swab Surveillance Cultures of Hematopoietic Stem Cell Recipients and Evaluation of Post-Transplant Bloodstream Infections: A Cross-Sectional Study from A Tertiary Care Teaching Hospital of Kolkata, India National Journal of Medical Research Bone marrow cell transplantation Stool surveillance culture Drug Resistance Bacteremia |
| title | Pathogenic Menace of MDR Bacteria in Stool and Throat Swab Surveillance Cultures of Hematopoietic Stem Cell Recipients and Evaluation of Post-Transplant Bloodstream Infections: A Cross-Sectional Study from A Tertiary Care Teaching Hospital of Kolkata, India |
| title_full | Pathogenic Menace of MDR Bacteria in Stool and Throat Swab Surveillance Cultures of Hematopoietic Stem Cell Recipients and Evaluation of Post-Transplant Bloodstream Infections: A Cross-Sectional Study from A Tertiary Care Teaching Hospital of Kolkata, India |
| title_fullStr | Pathogenic Menace of MDR Bacteria in Stool and Throat Swab Surveillance Cultures of Hematopoietic Stem Cell Recipients and Evaluation of Post-Transplant Bloodstream Infections: A Cross-Sectional Study from A Tertiary Care Teaching Hospital of Kolkata, India |
| title_full_unstemmed | Pathogenic Menace of MDR Bacteria in Stool and Throat Swab Surveillance Cultures of Hematopoietic Stem Cell Recipients and Evaluation of Post-Transplant Bloodstream Infections: A Cross-Sectional Study from A Tertiary Care Teaching Hospital of Kolkata, India |
| title_short | Pathogenic Menace of MDR Bacteria in Stool and Throat Swab Surveillance Cultures of Hematopoietic Stem Cell Recipients and Evaluation of Post-Transplant Bloodstream Infections: A Cross-Sectional Study from A Tertiary Care Teaching Hospital of Kolkata, India |
| title_sort | pathogenic menace of mdr bacteria in stool and throat swab surveillance cultures of hematopoietic stem cell recipients and evaluation of post transplant bloodstream infections a cross sectional study from a tertiary care teaching hospital of kolkata india |
| topic | Bone marrow cell transplantation Stool surveillance culture Drug Resistance Bacteremia |
| url | https://njmr.in/index.php/file/article/view/1035 |
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