The effect of Jian Gan powder on the proliferation, migration and polarization of macrophages and relative mechanism
Context Jian Gan powder (JGP) is a Chinese medicine compound comprised ginseng, Radix Paeoniae Alba, Radix Astragali, Salvia miltiorrhiza, Yujin, Rhizoma Cyperi, Fructus aurantii, Sophora flavescens, Yinchen, Bupleurum and licorice.Objective This study explored the inhibitory effects, polarization a...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Pharmaceutical Biology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/13880209.2024.2309864 |
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| author | Kun Li Xue Zheng Jian Zhang Zhanpeng Yan Yu Ji Fei Ge Fangshi Zhu |
| author_facet | Kun Li Xue Zheng Jian Zhang Zhanpeng Yan Yu Ji Fei Ge Fangshi Zhu |
| author_sort | Kun Li |
| collection | DOAJ |
| description | Context Jian Gan powder (JGP) is a Chinese medicine compound comprised ginseng, Radix Paeoniae Alba, Radix Astragali, Salvia miltiorrhiza, Yujin, Rhizoma Cyperi, Fructus aurantii, Sophora flavescens, Yinchen, Bupleurum and licorice.Objective This study explored the inhibitory effects, polarization and potential mechanisms associated with JGP in macrophages.Materials and methods RAW264.7 cells were randomly divided into six groups for 24 h: control, lipopolysaccharide (LPS), overexpression, 1% JGP, 2% JGP, 4% JGP, 8% JGP and 16% JGP. The effects of JGP on RAW264.7 cell proliferation were assessed using colony formation assays and cell counting kit-8 (CCK-8) assays. The Transwell assay was used to evaluate its impact on RAW264.7 cell migration. Moreover, we analysed the interleukin-6 (IL-6)/signal transducer and activator of the transcription 3 (IL-6/STAT3) signaling pathway using quantitative real-time PCR and Western blotting. Furthermore, we examined the M1/M2 polarization levels.Results Unlike LPS stimulation, JGP serum treatment markedly suppressed macrophage proliferation and migration capacity, while STAT3 overexpression enhanced RAW264.7 cell proliferation and migration. JGP inhibited the proliferation and migration of RAW264.7 cells by attenuating the IL-6/STAT3 signaling pathway. Furthermore, it inhibited macrophage M1 polarization, promoting M2 polarization.Discussion and conclusions JGP effectively suppressed the cellular function of RAW264.7 cells by down-regulating the IL-6/STAT3 signaling pathway and modulating macrophage M1/M2 polarization. These findings provide valuable theoretical and experimental basis for considering the potential clinical application of JGP in the treatment of immune-mediated liver injury in clinical practice. |
| format | Article |
| id | doaj-art-684bf09af02e425f94ee6eb855e3834a |
| institution | OA Journals |
| issn | 1388-0209 1744-5116 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj-art-684bf09af02e425f94ee6eb855e3834a2025-08-20T02:20:52ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162024-12-0162116216910.1080/13880209.2024.2309864The effect of Jian Gan powder on the proliferation, migration and polarization of macrophages and relative mechanismKun Li0Xue Zheng1Jian Zhang2Zhanpeng Yan3Yu Ji4Fei Ge5Fangshi Zhu6Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, PR ChinaAffiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, PR ChinaCollege of Pharmaceutical Science, Soochow University, Suzhou, PR ChinaAffiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, PR ChinaDepartment of Spleen and Stomach Diseases, Hai’an Traditional Chinese Medicine Hospital, Nantong, PR ChinaDepartment of Spleen and Stomach Diseases, Hai’an Traditional Chinese Medicine Hospital, Nantong, PR ChinaAffiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, PR ChinaContext Jian Gan powder (JGP) is a Chinese medicine compound comprised ginseng, Radix Paeoniae Alba, Radix Astragali, Salvia miltiorrhiza, Yujin, Rhizoma Cyperi, Fructus aurantii, Sophora flavescens, Yinchen, Bupleurum and licorice.Objective This study explored the inhibitory effects, polarization and potential mechanisms associated with JGP in macrophages.Materials and methods RAW264.7 cells were randomly divided into six groups for 24 h: control, lipopolysaccharide (LPS), overexpression, 1% JGP, 2% JGP, 4% JGP, 8% JGP and 16% JGP. The effects of JGP on RAW264.7 cell proliferation were assessed using colony formation assays and cell counting kit-8 (CCK-8) assays. The Transwell assay was used to evaluate its impact on RAW264.7 cell migration. Moreover, we analysed the interleukin-6 (IL-6)/signal transducer and activator of the transcription 3 (IL-6/STAT3) signaling pathway using quantitative real-time PCR and Western blotting. Furthermore, we examined the M1/M2 polarization levels.Results Unlike LPS stimulation, JGP serum treatment markedly suppressed macrophage proliferation and migration capacity, while STAT3 overexpression enhanced RAW264.7 cell proliferation and migration. JGP inhibited the proliferation and migration of RAW264.7 cells by attenuating the IL-6/STAT3 signaling pathway. Furthermore, it inhibited macrophage M1 polarization, promoting M2 polarization.Discussion and conclusions JGP effectively suppressed the cellular function of RAW264.7 cells by down-regulating the IL-6/STAT3 signaling pathway and modulating macrophage M1/M2 polarization. These findings provide valuable theoretical and experimental basis for considering the potential clinical application of JGP in the treatment of immune-mediated liver injury in clinical practice.https://www.tandfonline.com/doi/10.1080/13880209.2024.2309864JGPRAW264.7STAT3M1/M2 polarization |
| spellingShingle | Kun Li Xue Zheng Jian Zhang Zhanpeng Yan Yu Ji Fei Ge Fangshi Zhu The effect of Jian Gan powder on the proliferation, migration and polarization of macrophages and relative mechanism Pharmaceutical Biology JGP RAW264.7 STAT3 M1/M2 polarization |
| title | The effect of Jian Gan powder on the proliferation, migration and polarization of macrophages and relative mechanism |
| title_full | The effect of Jian Gan powder on the proliferation, migration and polarization of macrophages and relative mechanism |
| title_fullStr | The effect of Jian Gan powder on the proliferation, migration and polarization of macrophages and relative mechanism |
| title_full_unstemmed | The effect of Jian Gan powder on the proliferation, migration and polarization of macrophages and relative mechanism |
| title_short | The effect of Jian Gan powder on the proliferation, migration and polarization of macrophages and relative mechanism |
| title_sort | effect of jian gan powder on the proliferation migration and polarization of macrophages and relative mechanism |
| topic | JGP RAW264.7 STAT3 M1/M2 polarization |
| url | https://www.tandfonline.com/doi/10.1080/13880209.2024.2309864 |
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