Detection of Efflux Pump Using Ethidium Bromide-Agar Cartwheel Method in Acinetobacter baumannii Clinical Isolates
Introduction: In the past decade, multidrug-resistant Acinetobacter baumannii has become one of the most critical challenges in treating infected patients. The AdeABC efflux pump is the most important among the various resistance mechanisms. This pump can force various antibiotics and ethidium br...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Pasteur Institute of Iran
2022-03-01
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| Series: | Journal of Medical Microbiology and Infectious Diseases |
| Subjects: | |
| Online Access: | https://jommid.pasteur.ac.ir/article-1-410-en.html |
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| Summary: | Introduction: In the past decade, multidrug-resistant Acinetobacter baumannii
has become one of the most critical challenges in treating infected patients. The
AdeABC efflux pump is the most important among the various resistance
mechanisms. This pump can force various antibiotics and ethidium bromide out
of the bacterial cell to the surrounding environment. Methods: In this study,
nine A. baumannii clinical isolates were isolated and identified using different
biochemicals (catalase, oxidase, TSI, hemolysis, growth at 44°C, and indole)
and molecular (blaOxa51 gene) tests. Following the antibiogram test, the
antibiotic resistance changes in the isolates in the presence and absence of efflux
pump inhibitor (CCCP) were determined for tetracycline, and ciprofloxacin
AdeABC efflux pump genes, including adeA, adeB, and adeC, were amplified
by PCR. Finally, the presence of the AdeABC efflux pump was investigated
using the agar ethidium-bromide cartwheel method (AEBCM). Results:
According to the antibiogram test, all isolates were MDR. In the presence of
efflux pump inhibitor, a reduced resistance for tetracycline was observed, but
not for ciprofloxacin. The AdeABC efflux pump genes were detected in all
isolates. An increase in the AdeABC pump activity in four isolates was
confirmed using AEBCM. Conclusion: AEBCM, a fast and convenient tool for
assessing the ethidium bromide secretion in various bacteria, provides a quick
diagnosis and treatment of multidrug-resistant bacteria. |
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| ISSN: | 2345-5349 2345-5330 |