The vine tea flavonoids extraction by ultrasound-enzyme-assisted and its consequences for antioxidant efficacy and gut microbiota in rats

Vine tea is rich in flavonoids, and this study explored their high-value utilization of vine tea flavonoids (VEF). Usingresponse surface methodology (RSM), optimal ultrasound-enzymatic extraction conditions were determined: 75% ethanol, 1:30 solidliquidratio, 56 min ultrasonication, and enzyme conce...

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Main Authors: Hao Shi, Meng-ling Qin, Qing-yuan Liu, Tao Yang, Yu-ling Hu, Yun Wang, Jing Wang
Format: Article
Language:English
Published: Taylor & Francis Group 2025-12-01
Series:Food and Agricultural Immunology
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Online Access:https://www.tandfonline.com/doi/10.1080/09540105.2025.2470131
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author Hao Shi
Meng-ling Qin
Qing-yuan Liu
Tao Yang
Yu-ling Hu
Yun Wang
Jing Wang
author_facet Hao Shi
Meng-ling Qin
Qing-yuan Liu
Tao Yang
Yu-ling Hu
Yun Wang
Jing Wang
author_sort Hao Shi
collection DOAJ
description Vine tea is rich in flavonoids, and this study explored their high-value utilization of vine tea flavonoids (VEF). Usingresponse surface methodology (RSM), optimal ultrasound-enzymatic extraction conditions were determined: 75% ethanol, 1:30 solidliquidratio, 56 min ultrasonication, and enzyme concentrations of 2.1% papain, 2.3% pectinase, and 2.6% cellulase, achieving a 25.10%VEF yield. Purified VEF (PVEF) exhibited superior antioxidant activities, including in vitro, in vivo, and gut microbe-mediated effects.PVEF showed higher free radical scavenging and anti-lipid peroxidation than VC. Additionally, PVEF also enhanced beneficial gutbacteria, GSH-Px activity, and related gene (Gpx1, Gclm) and protein levels while reducing liver ROS in rats, outperforming the Xue ZhiKang group. Specifically, the H-PVEF group showed 34.98% and 49.47% higher GSH-Px activity than the control and model groups,respectively. In conclusion, ultrasound-enzyme-assisted extraction significantly boosts VEF yield, and PVEF demonstrates potentantioxidant properties, supporting its potential in functional foods and pharmaceuticals.
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issn 0954-0105
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publishDate 2025-12-01
publisher Taylor & Francis Group
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spelling doaj-art-68404ef52c904561bcd45d07018e58aa2025-08-20T03:12:24ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432025-12-0136110.1080/09540105.2025.2470131The vine tea flavonoids extraction by ultrasound-enzyme-assisted and its consequences for antioxidant efficacy and gut microbiota in ratsHao Shi0Meng-ling Qin1Qing-yuan Liu2Tao Yang3Yu-ling Hu4Yun Wang5Jing Wang6College of Agriculture and Forestry Technology, Hunan Applied Technology University, Changde, People’s Republic of ChinaCollege of Agriculture and Forestry Technology, Hunan Applied Technology University, Changde, People’s Republic of ChinaCollege of Agriculture and Forestry Technology, Hunan Applied Technology University, Changde, People’s Republic of ChinaCollege of Agriculture and Forestry Technology, Hunan Applied Technology University, Changde, People’s Republic of ChinaCollege of Agriculture and Forestry Technology, Hunan Applied Technology University, Changde, People’s Republic of ChinaCollege of Life and Environmental Sciences, Hunan university of Arts and Sciences, Changde, People’s Republic of ChinaCollege of Agriculture and Forestry Technology, Hunan Applied Technology University, Changde, People’s Republic of ChinaVine tea is rich in flavonoids, and this study explored their high-value utilization of vine tea flavonoids (VEF). Usingresponse surface methodology (RSM), optimal ultrasound-enzymatic extraction conditions were determined: 75% ethanol, 1:30 solidliquidratio, 56 min ultrasonication, and enzyme concentrations of 2.1% papain, 2.3% pectinase, and 2.6% cellulase, achieving a 25.10%VEF yield. Purified VEF (PVEF) exhibited superior antioxidant activities, including in vitro, in vivo, and gut microbe-mediated effects.PVEF showed higher free radical scavenging and anti-lipid peroxidation than VC. Additionally, PVEF also enhanced beneficial gutbacteria, GSH-Px activity, and related gene (Gpx1, Gclm) and protein levels while reducing liver ROS in rats, outperforming the Xue ZhiKang group. Specifically, the H-PVEF group showed 34.98% and 49.47% higher GSH-Px activity than the control and model groups,respectively. In conclusion, ultrasound-enzyme-assisted extraction significantly boosts VEF yield, and PVEF demonstrates potentantioxidant properties, supporting its potential in functional foods and pharmaceuticals.https://www.tandfonline.com/doi/10.1080/09540105.2025.2470131Vine teaFlavonoidsExtractionRatAntioxidant activityGut microbiota
spellingShingle Hao Shi
Meng-ling Qin
Qing-yuan Liu
Tao Yang
Yu-ling Hu
Yun Wang
Jing Wang
The vine tea flavonoids extraction by ultrasound-enzyme-assisted and its consequences for antioxidant efficacy and gut microbiota in rats
Food and Agricultural Immunology
Vine tea
Flavonoids
Extraction
Rat
Antioxidant activity
Gut microbiota
title The vine tea flavonoids extraction by ultrasound-enzyme-assisted and its consequences for antioxidant efficacy and gut microbiota in rats
title_full The vine tea flavonoids extraction by ultrasound-enzyme-assisted and its consequences for antioxidant efficacy and gut microbiota in rats
title_fullStr The vine tea flavonoids extraction by ultrasound-enzyme-assisted and its consequences for antioxidant efficacy and gut microbiota in rats
title_full_unstemmed The vine tea flavonoids extraction by ultrasound-enzyme-assisted and its consequences for antioxidant efficacy and gut microbiota in rats
title_short The vine tea flavonoids extraction by ultrasound-enzyme-assisted and its consequences for antioxidant efficacy and gut microbiota in rats
title_sort vine tea flavonoids extraction by ultrasound enzyme assisted and its consequences for antioxidant efficacy and gut microbiota in rats
topic Vine tea
Flavonoids
Extraction
Rat
Antioxidant activity
Gut microbiota
url https://www.tandfonline.com/doi/10.1080/09540105.2025.2470131
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