Capillary Dilation and Rarefaction Are Correlated with Intracapillary Inflammation in Antibody-Mediated Rejection
Antibody-mediated rejection (ABMR) remains one of the major causes of graft loss after renal transplantation. It is dominated by endothelial damage in microcirculation. Clarifying the mechanism of microcirculating damage is obviously a key step to understand the pathogenesis of ABMR. Here we charact...
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Wiley
2014-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2014/582902 |
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| author | Xue Li Qiquan Sun Mingchao Zhang Kenan Xie Jinsong Chen Zhihong Liu |
| author_facet | Xue Li Qiquan Sun Mingchao Zhang Kenan Xie Jinsong Chen Zhihong Liu |
| author_sort | Xue Li |
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| description | Antibody-mediated rejection (ABMR) remains one of the major causes of graft loss after renal transplantation. It is dominated by endothelial damage in microcirculation. Clarifying the mechanism of microcirculating damage is obviously a key step to understand the pathogenesis of ABMR. Here we characterized capillary variation in ABMR and its possible mechanisms. Compared with T cell-mediated rejection and stable grafts, there was a significant dilation and rarefaction in peritubular capillaries (PTCs) of the ABMR group; Image-Pro Plus revealed a significantly larger intra-PTC area. Interestingly, the dilation of PTCs was strongly correlated with the intra-PTC cell counting. Moreover, peritubular capillary inflammation is correlated with in situ T-bet expression, and there was a good correlation between the intra-PTC expression of T-bet and the PTC diameter. HIF-1α up-regulation could be observed in ABMR but it was not necessary for capillary dilation. In general, ABMR is characterized with early capillary dilation and rarefaction; our data confirmed that the dilation is strongly correlated with intracapillary inflammation, which in turn is correlated with in situ T-bet expression. T-bet plays an important role in the development of microcirculating injury, and thus it is a potential target for the treatment of ABMR. |
| format | Article |
| id | doaj-art-683f4a65e99e4fd6a9601a7d2b893ea1 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
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| series | Journal of Immunology Research |
| spelling | doaj-art-683f4a65e99e4fd6a9601a7d2b893ea12025-08-20T03:34:04ZengWileyJournal of Immunology Research2314-88612314-71562014-01-01201410.1155/2014/582902582902Capillary Dilation and Rarefaction Are Correlated with Intracapillary Inflammation in Antibody-Mediated RejectionXue Li0Qiquan Sun1Mingchao Zhang2Kenan Xie3Jinsong Chen4Zhihong Liu5National Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University Clinical School of Medicine, 305 East Zhong Shan Road, Nanjing 210002, ChinaDepartment of Renal Transplantation, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510760, ChinaNational Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University Clinical School of Medicine, 305 East Zhong Shan Road, Nanjing 210002, ChinaNational Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University Clinical School of Medicine, 305 East Zhong Shan Road, Nanjing 210002, ChinaNational Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University Clinical School of Medicine, 305 East Zhong Shan Road, Nanjing 210002, ChinaNational Clinical Research Center of Kidney Diseases, Jinling Hospital, Nanjing University Clinical School of Medicine, 305 East Zhong Shan Road, Nanjing 210002, ChinaAntibody-mediated rejection (ABMR) remains one of the major causes of graft loss after renal transplantation. It is dominated by endothelial damage in microcirculation. Clarifying the mechanism of microcirculating damage is obviously a key step to understand the pathogenesis of ABMR. Here we characterized capillary variation in ABMR and its possible mechanisms. Compared with T cell-mediated rejection and stable grafts, there was a significant dilation and rarefaction in peritubular capillaries (PTCs) of the ABMR group; Image-Pro Plus revealed a significantly larger intra-PTC area. Interestingly, the dilation of PTCs was strongly correlated with the intra-PTC cell counting. Moreover, peritubular capillary inflammation is correlated with in situ T-bet expression, and there was a good correlation between the intra-PTC expression of T-bet and the PTC diameter. HIF-1α up-regulation could be observed in ABMR but it was not necessary for capillary dilation. In general, ABMR is characterized with early capillary dilation and rarefaction; our data confirmed that the dilation is strongly correlated with intracapillary inflammation, which in turn is correlated with in situ T-bet expression. T-bet plays an important role in the development of microcirculating injury, and thus it is a potential target for the treatment of ABMR.http://dx.doi.org/10.1155/2014/582902 |
| spellingShingle | Xue Li Qiquan Sun Mingchao Zhang Kenan Xie Jinsong Chen Zhihong Liu Capillary Dilation and Rarefaction Are Correlated with Intracapillary Inflammation in Antibody-Mediated Rejection Journal of Immunology Research |
| title | Capillary Dilation and Rarefaction Are Correlated with Intracapillary Inflammation in Antibody-Mediated Rejection |
| title_full | Capillary Dilation and Rarefaction Are Correlated with Intracapillary Inflammation in Antibody-Mediated Rejection |
| title_fullStr | Capillary Dilation and Rarefaction Are Correlated with Intracapillary Inflammation in Antibody-Mediated Rejection |
| title_full_unstemmed | Capillary Dilation and Rarefaction Are Correlated with Intracapillary Inflammation in Antibody-Mediated Rejection |
| title_short | Capillary Dilation and Rarefaction Are Correlated with Intracapillary Inflammation in Antibody-Mediated Rejection |
| title_sort | capillary dilation and rarefaction are correlated with intracapillary inflammation in antibody mediated rejection |
| url | http://dx.doi.org/10.1155/2014/582902 |
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