Histopathologic observations in a coccidiosis model of Eimeria tenella
Abstract Background Species of the genus Eimeria cause coccidiosis in chickens, resulting in a huge burden to the poultry industry worldwide. Eimeria tenella is one of the most prevalent chicken coccidia in China, and E. tenella infection causes hemorrhagic cecitis. Methods Using an established mode...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-12-01
|
| Series: | Animal Models and Experimental Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ame2.12463 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850238677183102976 |
|---|---|
| author | Zhixin Sun Linlin Chen Mengyu Lai Xixi Zhang Jie Li Zhiping Li Dongming Yang Mengyang Zhao Dongdong Wang Pei Wen Fengting Gou Yuexin Dai Yilan Ji Wen Li Deming Zhao Xianyong Liu Lifeng Yang |
| author_facet | Zhixin Sun Linlin Chen Mengyu Lai Xixi Zhang Jie Li Zhiping Li Dongming Yang Mengyang Zhao Dongdong Wang Pei Wen Fengting Gou Yuexin Dai Yilan Ji Wen Li Deming Zhao Xianyong Liu Lifeng Yang |
| author_sort | Zhixin Sun |
| collection | DOAJ |
| description | Abstract Background Species of the genus Eimeria cause coccidiosis in chickens, resulting in a huge burden to the poultry industry worldwide. Eimeria tenella is one of the most prevalent chicken coccidia in China, and E. tenella infection causes hemorrhagic cecitis. Methods Using an established model of coccidiosis in chickens combined with necropsy, imaging of pathological tissue sections, and other techniques, we evaluated the gross and microscopic lesions of cecal tissue within 15 days after inoculation with sporulated oocysts and described the endogenetic developmental process and relationship between E. tenella infection and enteritis development in chickens. Results We observed three generations of merogony and gamogony in E. tenella. We observed gross lesions in the cecum from 84 hpi (hours post inoculation) and microscopic lesions from 60 hpi. The lesions in the cecum mainly exhibited hemorrhagic enteritis. Their severity increased with the onset of the second generation of merogony. The lesions began to alleviate by the end of the endogenous stages of E. tenella. Conclusion We show, for the first time, the complete observation of a series of changes in enteritis caused by 5 × 103 E. tenella oocysts. This study provides reference materials for E. tenella research and pathological diagnosis. |
| format | Article |
| id | doaj-art-6831cb7fe125490da48765f168b126d2 |
| institution | OA Journals |
| issn | 2576-2095 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Animal Models and Experimental Medicine |
| spelling | doaj-art-6831cb7fe125490da48765f168b126d22025-08-20T02:01:24ZengWileyAnimal Models and Experimental Medicine2576-20952024-12-017689390310.1002/ame2.12463Histopathologic observations in a coccidiosis model of Eimeria tenellaZhixin Sun0Linlin Chen1Mengyu Lai2Xixi Zhang3Jie Li4Zhiping Li5Dongming Yang6Mengyang Zhao7Dongdong Wang8Pei Wen9Fengting Gou10Yuexin Dai11Yilan Ji12Wen Li13Deming Zhao14Xianyong Liu15Lifeng Yang16National Key Laboratory of Veterinary Public Health and Safety, Key Laboratory of Animal Epidemiology of the Ministry of Agriculture and Rural Affairs, National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine China Agricultural University Beijing ChinaNational Key Laboratory of Veterinary Public Health Security, Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, National Animal Protozoa Laboratory and College of Veterinary Medicine China Agricultural University Beijing ChinaNational Key Laboratory of Veterinary Public Health and Safety, Key Laboratory of Animal Epidemiology of the Ministry of Agriculture and Rural Affairs, National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine China Agricultural University Beijing ChinaNational Key Laboratory of Veterinary Public Health and Safety, Key Laboratory of Animal Epidemiology of the Ministry of Agriculture and Rural Affairs, National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine China Agricultural University Beijing ChinaNational Key Laboratory of Veterinary Public Health and Safety, Key Laboratory of Animal Epidemiology of the Ministry of Agriculture and Rural Affairs, National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine China Agricultural University Beijing ChinaNational Key Laboratory of Veterinary Public Health and Safety, Key Laboratory of Animal Epidemiology of the Ministry of Agriculture and Rural Affairs, National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine China Agricultural University Beijing ChinaNational Key Laboratory of Veterinary Public Health and Safety, Key Laboratory of Animal Epidemiology of the Ministry of Agriculture and Rural Affairs, National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine China Agricultural University Beijing ChinaNational Key Laboratory of Veterinary Public Health and Safety, Key Laboratory of Animal Epidemiology of the Ministry of Agriculture and Rural Affairs, National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine China Agricultural University Beijing ChinaNational Key Laboratory of Veterinary Public Health and Safety, Key Laboratory of Animal Epidemiology of the Ministry of Agriculture and Rural Affairs, National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine China Agricultural University Beijing ChinaNational Key Laboratory of Veterinary Public Health and Safety, Key Laboratory of Animal Epidemiology of the Ministry of Agriculture and Rural Affairs, National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine China Agricultural University Beijing ChinaNational Key Laboratory of Veterinary Public Health and Safety, Key Laboratory of Animal Epidemiology of the Ministry of Agriculture and Rural Affairs, National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine China Agricultural University Beijing ChinaNational Key Laboratory of Veterinary Public Health and Safety, Key Laboratory of Animal Epidemiology of the Ministry of Agriculture and Rural Affairs, National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine China Agricultural University Beijing ChinaNational Key Laboratory of Veterinary Public Health and Safety, Key Laboratory of Animal Epidemiology of the Ministry of Agriculture and Rural Affairs, National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine China Agricultural University Beijing ChinaNational Key Laboratory of Veterinary Public Health and Safety, Key Laboratory of Animal Epidemiology of the Ministry of Agriculture and Rural Affairs, National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine China Agricultural University Beijing ChinaNational Key Laboratory of Veterinary Public Health and Safety, Key Laboratory of Animal Epidemiology of the Ministry of Agriculture and Rural Affairs, National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine China Agricultural University Beijing ChinaNational Key Laboratory of Veterinary Public Health Security, Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture, National Animal Protozoa Laboratory and College of Veterinary Medicine China Agricultural University Beijing ChinaNational Key Laboratory of Veterinary Public Health and Safety, Key Laboratory of Animal Epidemiology of the Ministry of Agriculture and Rural Affairs, National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine China Agricultural University Beijing ChinaAbstract Background Species of the genus Eimeria cause coccidiosis in chickens, resulting in a huge burden to the poultry industry worldwide. Eimeria tenella is one of the most prevalent chicken coccidia in China, and E. tenella infection causes hemorrhagic cecitis. Methods Using an established model of coccidiosis in chickens combined with necropsy, imaging of pathological tissue sections, and other techniques, we evaluated the gross and microscopic lesions of cecal tissue within 15 days after inoculation with sporulated oocysts and described the endogenetic developmental process and relationship between E. tenella infection and enteritis development in chickens. Results We observed three generations of merogony and gamogony in E. tenella. We observed gross lesions in the cecum from 84 hpi (hours post inoculation) and microscopic lesions from 60 hpi. The lesions in the cecum mainly exhibited hemorrhagic enteritis. Their severity increased with the onset of the second generation of merogony. The lesions began to alleviate by the end of the endogenous stages of E. tenella. Conclusion We show, for the first time, the complete observation of a series of changes in enteritis caused by 5 × 103 E. tenella oocysts. This study provides reference materials for E. tenella research and pathological diagnosis.https://doi.org/10.1002/ame2.12463cecitischickenEimeria tenellagamontshistopathologymeronts |
| spellingShingle | Zhixin Sun Linlin Chen Mengyu Lai Xixi Zhang Jie Li Zhiping Li Dongming Yang Mengyang Zhao Dongdong Wang Pei Wen Fengting Gou Yuexin Dai Yilan Ji Wen Li Deming Zhao Xianyong Liu Lifeng Yang Histopathologic observations in a coccidiosis model of Eimeria tenella Animal Models and Experimental Medicine cecitis chicken Eimeria tenella gamonts histopathology meronts |
| title | Histopathologic observations in a coccidiosis model of Eimeria tenella |
| title_full | Histopathologic observations in a coccidiosis model of Eimeria tenella |
| title_fullStr | Histopathologic observations in a coccidiosis model of Eimeria tenella |
| title_full_unstemmed | Histopathologic observations in a coccidiosis model of Eimeria tenella |
| title_short | Histopathologic observations in a coccidiosis model of Eimeria tenella |
| title_sort | histopathologic observations in a coccidiosis model of eimeria tenella |
| topic | cecitis chicken Eimeria tenella gamonts histopathology meronts |
| url | https://doi.org/10.1002/ame2.12463 |
| work_keys_str_mv | AT zhixinsun histopathologicobservationsinacoccidiosismodelofeimeriatenella AT linlinchen histopathologicobservationsinacoccidiosismodelofeimeriatenella AT mengyulai histopathologicobservationsinacoccidiosismodelofeimeriatenella AT xixizhang histopathologicobservationsinacoccidiosismodelofeimeriatenella AT jieli histopathologicobservationsinacoccidiosismodelofeimeriatenella AT zhipingli histopathologicobservationsinacoccidiosismodelofeimeriatenella AT dongmingyang histopathologicobservationsinacoccidiosismodelofeimeriatenella AT mengyangzhao histopathologicobservationsinacoccidiosismodelofeimeriatenella AT dongdongwang histopathologicobservationsinacoccidiosismodelofeimeriatenella AT peiwen histopathologicobservationsinacoccidiosismodelofeimeriatenella AT fengtinggou histopathologicobservationsinacoccidiosismodelofeimeriatenella AT yuexindai histopathologicobservationsinacoccidiosismodelofeimeriatenella AT yilanji histopathologicobservationsinacoccidiosismodelofeimeriatenella AT wenli histopathologicobservationsinacoccidiosismodelofeimeriatenella AT demingzhao histopathologicobservationsinacoccidiosismodelofeimeriatenella AT xianyongliu histopathologicobservationsinacoccidiosismodelofeimeriatenella AT lifengyang histopathologicobservationsinacoccidiosismodelofeimeriatenella |