Metabolic Alterations in Crassostrea Gigas After Feeding Selenium-Enriched Yeast Based on Transcriptomic Analysis
This study explores the effects of selenium-enriched yeast supplementation on growth-related and immune-related gene expression in <i>C. gigas</i>, aiming to support feed optimization in oyster aquaculture. Selenium, an essential trace element, is vital for growth, immune function, and m...
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2025-07-01
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| author | Yancheng Zhao Xiaojing Jiang Liming Jiang Yongjie Wang Cuiju Cui Xiumei Liu Zan Li Weijun Wang Jianmin Yang |
| author_facet | Yancheng Zhao Xiaojing Jiang Liming Jiang Yongjie Wang Cuiju Cui Xiumei Liu Zan Li Weijun Wang Jianmin Yang |
| author_sort | Yancheng Zhao |
| collection | DOAJ |
| description | This study explores the effects of selenium-enriched yeast supplementation on growth-related and immune-related gene expression in <i>C. gigas</i>, aiming to support feed optimization in oyster aquaculture. Selenium, an essential trace element, is vital for growth, immune function, and metabolism in animals. Selenium-enriched yeast, an organic form, offers superior bioavailability, enabling efficient absorption and utilization. <i>C. gigas</i>, a commercially significant marine shellfish, is rich in protein and nutrients, but the effects of selenium on mollusks remain insufficiently explored. In this study, oysters were divided into three groups: a control group without selenium (THNP), a 2 ppm selenium group (THMP), and a 4 ppm selenium group (THHP). Transcriptome sequencing yielded 388,679,026 clean reads. GO and KEGG enrichment analyses identified key metabolic signaling pathways, and a PPI analysis was performed on the translation products of DEGs involved in the KEGG pathways. qRT-PCR validated the expression of principal DEGs. The combined results of enrichment and PPI analyses highlighted pathways such as glutathione metabolism and collagen signaling. Additionally, three hub genes—<i>FASN</i>, <i>HRAS</i>, and <i>ABCG5</i>—were identified as central to the selenium response. These findings enhance the understanding of selenium’s molecular impact on oysters and support its application in aquaculture. |
| format | Article |
| id | doaj-art-6814ffef43b8422880b5b967092ca554 |
| institution | DOAJ |
| issn | 2079-7737 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-6814ffef43b8422880b5b967092ca5542025-08-20T03:13:39ZengMDPI AGBiology2079-77372025-07-0114789810.3390/biology14070898Metabolic Alterations in Crassostrea Gigas After Feeding Selenium-Enriched Yeast Based on Transcriptomic AnalysisYancheng Zhao0Xiaojing Jiang1Liming Jiang2Yongjie Wang3Cuiju Cui4Xiumei Liu5Zan Li6Weijun Wang7Jianmin Yang8School of Fisheries, Ludong University, Yantai 264025, ChinaYantai Campus, Binzhou Medical University, Yantai 264003, ChinaYantai Marine Economic Research Institute, Yantai 264003, ChinaSchool of Fisheries, Ludong University, Yantai 264025, ChinaSchool of Fisheries, Ludong University, Yantai 264025, ChinaCollege of Life Sciences, Yantai University, Yantai 264005, ChinaSchool of Fisheries, Ludong University, Yantai 264025, ChinaSchool of Fisheries, Ludong University, Yantai 264025, ChinaSchool of Fisheries, Ludong University, Yantai 264025, ChinaThis study explores the effects of selenium-enriched yeast supplementation on growth-related and immune-related gene expression in <i>C. gigas</i>, aiming to support feed optimization in oyster aquaculture. Selenium, an essential trace element, is vital for growth, immune function, and metabolism in animals. Selenium-enriched yeast, an organic form, offers superior bioavailability, enabling efficient absorption and utilization. <i>C. gigas</i>, a commercially significant marine shellfish, is rich in protein and nutrients, but the effects of selenium on mollusks remain insufficiently explored. In this study, oysters were divided into three groups: a control group without selenium (THNP), a 2 ppm selenium group (THMP), and a 4 ppm selenium group (THHP). Transcriptome sequencing yielded 388,679,026 clean reads. GO and KEGG enrichment analyses identified key metabolic signaling pathways, and a PPI analysis was performed on the translation products of DEGs involved in the KEGG pathways. qRT-PCR validated the expression of principal DEGs. The combined results of enrichment and PPI analyses highlighted pathways such as glutathione metabolism and collagen signaling. Additionally, three hub genes—<i>FASN</i>, <i>HRAS</i>, and <i>ABCG5</i>—were identified as central to the selenium response. These findings enhance the understanding of selenium’s molecular impact on oysters and support its application in aquaculture.https://www.mdpi.com/2079-7737/14/7/898<i>Crassostrea gigas</i>transcriptomicselenium-enriched yeastprotein-protein interaction network |
| spellingShingle | Yancheng Zhao Xiaojing Jiang Liming Jiang Yongjie Wang Cuiju Cui Xiumei Liu Zan Li Weijun Wang Jianmin Yang Metabolic Alterations in Crassostrea Gigas After Feeding Selenium-Enriched Yeast Based on Transcriptomic Analysis Biology <i>Crassostrea gigas</i> transcriptomic selenium-enriched yeast protein-protein interaction network |
| title | Metabolic Alterations in Crassostrea Gigas After Feeding Selenium-Enriched Yeast Based on Transcriptomic Analysis |
| title_full | Metabolic Alterations in Crassostrea Gigas After Feeding Selenium-Enriched Yeast Based on Transcriptomic Analysis |
| title_fullStr | Metabolic Alterations in Crassostrea Gigas After Feeding Selenium-Enriched Yeast Based on Transcriptomic Analysis |
| title_full_unstemmed | Metabolic Alterations in Crassostrea Gigas After Feeding Selenium-Enriched Yeast Based on Transcriptomic Analysis |
| title_short | Metabolic Alterations in Crassostrea Gigas After Feeding Selenium-Enriched Yeast Based on Transcriptomic Analysis |
| title_sort | metabolic alterations in crassostrea gigas after feeding selenium enriched yeast based on transcriptomic analysis |
| topic | <i>Crassostrea gigas</i> transcriptomic selenium-enriched yeast protein-protein interaction network |
| url | https://www.mdpi.com/2079-7737/14/7/898 |
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