Visualization through Magnetic Resonance Imaging of DNA Internalized Following “In Vivo” Electroporation
The ability to visualize plasmid DNA entrapment in muscle cells undergoing an “in vivo” electroporation treatment was investigated on BALB/c mice using a 7-T magnetic resonance imaging (MRI) scanner using the paramagnetic Gd–DOTA–spd complex as imaging reporter. Gd–DOTA–spd bears a tripositively cha...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2005-01-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.1162/15353500200504151 |
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| _version_ | 1841564462855749632 |
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| author | Simonetta Geninatti Crich Stefania Lanzardo Alessandro Barge Giovanna Esposito Lorenzo Tei Guido Forni Silvio Aime |
| author_facet | Simonetta Geninatti Crich Stefania Lanzardo Alessandro Barge Giovanna Esposito Lorenzo Tei Guido Forni Silvio Aime |
| author_sort | Simonetta Geninatti Crich |
| collection | DOAJ |
| description | The ability to visualize plasmid DNA entrapment in muscle cells undergoing an “in vivo” electroporation treatment was investigated on BALB/c mice using a 7-T magnetic resonance imaging (MRI) scanner using the paramagnetic Gd–DOTA–spd complex as imaging reporter. Gd–DOTA–spd bears a tripositively charged spermidine residue that yields a strong binding affinity toward the negatively charged DNA chain (6.4 kb, K a = 2.2 × 10 3 M −1 for approximately 2500 ± 500 binding sites). Cellular colocalization of Gd-DOTA-spd and plasmid DNA has been validated by histological analysis of excised treated muscle. In vivo MRI visualization of Gd-DOTA-spd distribution provides an excellent route to access the cellular entrapment of plasmid DNA upon applying an electroporation pulse. |
| format | Article |
| id | doaj-art-6811393f5211475f8bca390bf076b66f |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2005-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-6811393f5211475f8bca390bf076b66f2025-01-02T22:38:07ZengSAGE PublishingMolecular Imaging1536-01212005-01-01410.1162/1535350020050415110.1162_15353500200504151Visualization through Magnetic Resonance Imaging of DNA Internalized Following “In Vivo” ElectroporationSimonetta Geninatti CrichStefania LanzardoAlessandro BargeGiovanna EspositoLorenzo TeiGuido ForniSilvio AimeThe ability to visualize plasmid DNA entrapment in muscle cells undergoing an “in vivo” electroporation treatment was investigated on BALB/c mice using a 7-T magnetic resonance imaging (MRI) scanner using the paramagnetic Gd–DOTA–spd complex as imaging reporter. Gd–DOTA–spd bears a tripositively charged spermidine residue that yields a strong binding affinity toward the negatively charged DNA chain (6.4 kb, K a = 2.2 × 10 3 M −1 for approximately 2500 ± 500 binding sites). Cellular colocalization of Gd-DOTA-spd and plasmid DNA has been validated by histological analysis of excised treated muscle. In vivo MRI visualization of Gd-DOTA-spd distribution provides an excellent route to access the cellular entrapment of plasmid DNA upon applying an electroporation pulse.https://doi.org/10.1162/15353500200504151 |
| spellingShingle | Simonetta Geninatti Crich Stefania Lanzardo Alessandro Barge Giovanna Esposito Lorenzo Tei Guido Forni Silvio Aime Visualization through Magnetic Resonance Imaging of DNA Internalized Following “In Vivo” Electroporation Molecular Imaging |
| title | Visualization through Magnetic Resonance Imaging of DNA Internalized Following “In Vivo” Electroporation |
| title_full | Visualization through Magnetic Resonance Imaging of DNA Internalized Following “In Vivo” Electroporation |
| title_fullStr | Visualization through Magnetic Resonance Imaging of DNA Internalized Following “In Vivo” Electroporation |
| title_full_unstemmed | Visualization through Magnetic Resonance Imaging of DNA Internalized Following “In Vivo” Electroporation |
| title_short | Visualization through Magnetic Resonance Imaging of DNA Internalized Following “In Vivo” Electroporation |
| title_sort | visualization through magnetic resonance imaging of dna internalized following in vivo electroporation |
| url | https://doi.org/10.1162/15353500200504151 |
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