Visualization through Magnetic Resonance Imaging of DNA Internalized Following “In Vivo” Electroporation

The ability to visualize plasmid DNA entrapment in muscle cells undergoing an “in vivo” electroporation treatment was investigated on BALB/c mice using a 7-T magnetic resonance imaging (MRI) scanner using the paramagnetic Gd–DOTA–spd complex as imaging reporter. Gd–DOTA–spd bears a tripositively cha...

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Bibliographic Details
Main Authors: Simonetta Geninatti Crich, Stefania Lanzardo, Alessandro Barge, Giovanna Esposito, Lorenzo Tei, Guido Forni, Silvio Aime
Format: Article
Language:English
Published: SAGE Publishing 2005-01-01
Series:Molecular Imaging
Online Access:https://doi.org/10.1162/15353500200504151
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Summary:The ability to visualize plasmid DNA entrapment in muscle cells undergoing an “in vivo” electroporation treatment was investigated on BALB/c mice using a 7-T magnetic resonance imaging (MRI) scanner using the paramagnetic Gd–DOTA–spd complex as imaging reporter. Gd–DOTA–spd bears a tripositively charged spermidine residue that yields a strong binding affinity toward the negatively charged DNA chain (6.4 kb, K a = 2.2 × 10 3 M −1 for approximately 2500 ± 500 binding sites). Cellular colocalization of Gd-DOTA-spd and plasmid DNA has been validated by histological analysis of excised treated muscle. In vivo MRI visualization of Gd-DOTA-spd distribution provides an excellent route to access the cellular entrapment of plasmid DNA upon applying an electroporation pulse.
ISSN:1536-0121