Transmission Raman Spectroscopy for Inner Layers Chemical Analysis of Fresh Produce

Identification of chemical properties in the inner tissues of fresh produce would enable us to identify major issues plaguing the agriculture supply chain, like off-flavors and core rot since these are caused by or accompanied by known chemical elements. We show the development of transmission Raman...

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Main Author: Rani Arielly
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Sensors
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Online Access:https://www.mdpi.com/1424-8220/25/9/2805
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author Rani Arielly
author_facet Rani Arielly
author_sort Rani Arielly
collection DOAJ
description Identification of chemical properties in the inner tissues of fresh produce would enable us to identify major issues plaguing the agriculture supply chain, like off-flavors and core rot since these are caused by or accompanied by known chemical elements. We show the development of transmission Raman spectroscopy system for identifying these elements by addressing several issues: we located an optimal spectral window by conducting optical attenuation measurements and calculated the required LASER power in that range. For apple tissues, this optical window was found in the 700–950 nm range, and the required LASER power range was calculated to be in the 40–700 mW range. We also calculated that the optimal shifted-excitation Raman difference spectroscopy wavelengths should be separated by 0.7 nm in order to optimally produce narrow and high-intensity Raman peak features and eliminate the competing fluorescence signal. Finally, we provide a complete optical system design with exact optimal parameters. In contrast to other fields like pharmaceuticals and medicine, transmission Raman spectroscopy has not been applied extensively in agriculture. Therefore, this study fills a gap in that field’s applicability.
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spelling doaj-art-67ef8efffd7f4f29a14d85a47a9e4f6a2025-08-20T02:31:20ZengMDPI AGSensors1424-82202025-04-01259280510.3390/s25092805Transmission Raman Spectroscopy for Inner Layers Chemical Analysis of Fresh ProduceRani Arielly0Institute of Agricultural and Biosystems Engineering, Agricultural Research Organization–Volcani Institute, Rishon LeZion 7505101, IsraelIdentification of chemical properties in the inner tissues of fresh produce would enable us to identify major issues plaguing the agriculture supply chain, like off-flavors and core rot since these are caused by or accompanied by known chemical elements. We show the development of transmission Raman spectroscopy system for identifying these elements by addressing several issues: we located an optimal spectral window by conducting optical attenuation measurements and calculated the required LASER power in that range. For apple tissues, this optical window was found in the 700–950 nm range, and the required LASER power range was calculated to be in the 40–700 mW range. We also calculated that the optimal shifted-excitation Raman difference spectroscopy wavelengths should be separated by 0.7 nm in order to optimally produce narrow and high-intensity Raman peak features and eliminate the competing fluorescence signal. Finally, we provide a complete optical system design with exact optimal parameters. In contrast to other fields like pharmaceuticals and medicine, transmission Raman spectroscopy has not been applied extensively in agriculture. Therefore, this study fills a gap in that field’s applicability.https://www.mdpi.com/1424-8220/25/9/2805postharvestfood qualityfood safetyopticsRaman spectroscopySERDS
spellingShingle Rani Arielly
Transmission Raman Spectroscopy for Inner Layers Chemical Analysis of Fresh Produce
Sensors
postharvest
food quality
food safety
optics
Raman spectroscopy
SERDS
title Transmission Raman Spectroscopy for Inner Layers Chemical Analysis of Fresh Produce
title_full Transmission Raman Spectroscopy for Inner Layers Chemical Analysis of Fresh Produce
title_fullStr Transmission Raman Spectroscopy for Inner Layers Chemical Analysis of Fresh Produce
title_full_unstemmed Transmission Raman Spectroscopy for Inner Layers Chemical Analysis of Fresh Produce
title_short Transmission Raman Spectroscopy for Inner Layers Chemical Analysis of Fresh Produce
title_sort transmission raman spectroscopy for inner layers chemical analysis of fresh produce
topic postharvest
food quality
food safety
optics
Raman spectroscopy
SERDS
url https://www.mdpi.com/1424-8220/25/9/2805
work_keys_str_mv AT raniarielly transmissionramanspectroscopyforinnerlayerschemicalanalysisoffreshproduce