Intravitreal panitumumab and retinal pigment epithelium proliferation in laser-induced retinal degeneration in rabbits
PurposeThis study aims to examine the effect of intravitreally applied epidermal growth factor (EGF) receptor blocker panitumumab on the proliferation of retinal pigment epithelium cells (RPE) in an experimental model of localized retinal degeneration.MethodsThe experimental study included rabbits w...
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Frontiers Media S.A.
2025-08-01
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| Series: | Frontiers in Ophthalmology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fopht.2025.1641194/full |
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| author | Mukharram M. Bikbov Gyulli M. Kazakbaeva Songhomitra Panda-Jonas Songhomitra Panda-Jonas Songhomitra Panda-Jonas Iskander D. Valishin Aigul M. Nizamutdinova Jost B. Jonas Jost B. Jonas Jost B. Jonas Jost B. Jonas Jost B. Jonas Jost B. Jonas Jost B. Jonas |
| author_facet | Mukharram M. Bikbov Gyulli M. Kazakbaeva Songhomitra Panda-Jonas Songhomitra Panda-Jonas Songhomitra Panda-Jonas Iskander D. Valishin Aigul M. Nizamutdinova Jost B. Jonas Jost B. Jonas Jost B. Jonas Jost B. Jonas Jost B. Jonas Jost B. Jonas Jost B. Jonas |
| author_sort | Mukharram M. Bikbov |
| collection | DOAJ |
| description | PurposeThis study aims to examine the effect of intravitreally applied epidermal growth factor (EGF) receptor blocker panitumumab on the proliferation of retinal pigment epithelium cells (RPE) in an experimental model of localized retinal degeneration.MethodsThe experimental study included rabbits with age of 2 to 3 months and body weight of 2.5–3 kg and which were randomly distributed into a study group and control group. The right eyes received two retinal argon laser coagulation spots (500 mW; diameter, 100 μm; duration, 0.5 s), applied with an interval of 2 min at the same location close to the vascular streak in the posterior fundus region. For five times at 2-day intervals, the rabbits of the study group received intravitreal injections of 1 mg panitumumab (0.10 mL), and the rabbits of the control group had intravitreal injections of 0.10 mL Ringer’s solution. At baseline, at each time point of re-examination, and at study end, the animals were examined by fundus photography and optical coherence tomography of the laser spot.ResultsThe study included 19 rabbits (study group: 10 animals; control group: nine animals). After the third injection and at study end, the laser-induced area of depigmentation + hyperpigmentation combined did not vary significantly between the study group and the control group (1.43 ± 0.63 mm2 versus 1.63 ± 0.77 mm2; P = 0.56; and 1.37 ± 0.63 mm2 versus 1.61 ± 0.74 mm2; P = 0.46, respectively). At the same time points, the area with hyperpigmentation was significantly smaller in the study group than in the control group (0.16 ± 0.15 mm2 versus 0.80 ± 0.59 mm2; P = 0.01; and 0.14 ± 0.14 mm2 versus 0.70 ± 0.56 mm2; P = 0.02, respectively). At the same time points, the ratio of the hyperpigmented area to the combined depigmented + hyperpigmented area was significantly smaller in the study group than in the control group (0.11 ± 0.09 versus 0.43 ± 0.19 mm2; P < 0.001; and 0.10 ± 0.08 versus 0.35 ± 0.23mm2; P = 0.006, respectively)ConclusionsThese findings indicate that intravitreally administered panitumumab was associated with reduced subretinal hyperpigmentation in a laser-induced model of retinal injury. While this may reflect a modulation of the RPE response, including the potential suppression of RPE proliferation, further studies incorporating histological and molecular analyses are warranted to confirm its effect on subretinal fibrosis. |
| format | Article |
| id | doaj-art-67daee62da7f49908275ee8ae233d5d1 |
| institution | Kabale University |
| issn | 2674-0826 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Ophthalmology |
| spelling | doaj-art-67daee62da7f49908275ee8ae233d5d12025-08-20T03:59:40ZengFrontiers Media S.A.Frontiers in Ophthalmology2674-08262025-08-01510.3389/fopht.2025.16411941641194Intravitreal panitumumab and retinal pigment epithelium proliferation in laser-induced retinal degeneration in rabbitsMukharram M. Bikbov0Gyulli M. Kazakbaeva1Songhomitra Panda-Jonas2Songhomitra Panda-Jonas3Songhomitra Panda-Jonas4Iskander D. Valishin5Aigul M. Nizamutdinova6Jost B. Jonas7Jost B. Jonas8Jost B. Jonas9Jost B. Jonas10Jost B. Jonas11Jost B. Jonas12Jost B. Jonas13Ufa Eye Research Institute, Bashkir State Medical University, Ufa, RussiaUfa Eye Research Institute, Bashkir State Medical University, Ufa, RussiaDepartment of Ophthalmology, Medical Faculty Heidelberg, Heidelberg University, Heidelberg, GermanyL V Prasad Eye Institute, Hyderabad, Telangana, IndiaPrivatpraxis Prof Jonas und Dr Panda-Jonas, Heidelberg, GermanyUfa Eye Research Institute, Bashkir State Medical University, Ufa, RussiaUfa Eye Research Institute, Bashkir State Medical University, Ufa, RussiaL V Prasad Eye Institute, Hyderabad, Telangana, IndiaPrivatpraxis Prof Jonas und Dr Panda-Jonas, Heidelberg, GermanyRothschild Foundation Hospital, Institut Français de Myopie, Paris, FranceSingapore Eye Research Institute, Singapore National Eye Center, Singapore, SingaporeBeijing Visual Science and Translational Eye Research Institute (BERI), Beijing Tsinghua Changgung Hospital, Tsinghua Medicine, Tsinghua University, Beijing, ChinaDepartment of Ophthalmology and Visual Sciences, The Chinese University of Hong Kong, Hong Kong, Hong Kong SAR, ChinaNew York Eye and Ear Infirmary of Mount Sinai, Icahn School of Medicine at Mount Sinai, New York, NY, United StatesPurposeThis study aims to examine the effect of intravitreally applied epidermal growth factor (EGF) receptor blocker panitumumab on the proliferation of retinal pigment epithelium cells (RPE) in an experimental model of localized retinal degeneration.MethodsThe experimental study included rabbits with age of 2 to 3 months and body weight of 2.5–3 kg and which were randomly distributed into a study group and control group. The right eyes received two retinal argon laser coagulation spots (500 mW; diameter, 100 μm; duration, 0.5 s), applied with an interval of 2 min at the same location close to the vascular streak in the posterior fundus region. For five times at 2-day intervals, the rabbits of the study group received intravitreal injections of 1 mg panitumumab (0.10 mL), and the rabbits of the control group had intravitreal injections of 0.10 mL Ringer’s solution. At baseline, at each time point of re-examination, and at study end, the animals were examined by fundus photography and optical coherence tomography of the laser spot.ResultsThe study included 19 rabbits (study group: 10 animals; control group: nine animals). After the third injection and at study end, the laser-induced area of depigmentation + hyperpigmentation combined did not vary significantly between the study group and the control group (1.43 ± 0.63 mm2 versus 1.63 ± 0.77 mm2; P = 0.56; and 1.37 ± 0.63 mm2 versus 1.61 ± 0.74 mm2; P = 0.46, respectively). At the same time points, the area with hyperpigmentation was significantly smaller in the study group than in the control group (0.16 ± 0.15 mm2 versus 0.80 ± 0.59 mm2; P = 0.01; and 0.14 ± 0.14 mm2 versus 0.70 ± 0.56 mm2; P = 0.02, respectively). At the same time points, the ratio of the hyperpigmented area to the combined depigmented + hyperpigmented area was significantly smaller in the study group than in the control group (0.11 ± 0.09 versus 0.43 ± 0.19 mm2; P < 0.001; and 0.10 ± 0.08 versus 0.35 ± 0.23mm2; P = 0.006, respectively)ConclusionsThese findings indicate that intravitreally administered panitumumab was associated with reduced subretinal hyperpigmentation in a laser-induced model of retinal injury. While this may reflect a modulation of the RPE response, including the potential suppression of RPE proliferation, further studies incorporating histological and molecular analyses are warranted to confirm its effect on subretinal fibrosis.https://www.frontiersin.org/articles/10.3389/fopht.2025.1641194/fullpanitumumabepidermal growth factor receptor inhibitormacular degenerationretinal pigment epitheliummacular scarintravitreal medication |
| spellingShingle | Mukharram M. Bikbov Gyulli M. Kazakbaeva Songhomitra Panda-Jonas Songhomitra Panda-Jonas Songhomitra Panda-Jonas Iskander D. Valishin Aigul M. Nizamutdinova Jost B. Jonas Jost B. Jonas Jost B. Jonas Jost B. Jonas Jost B. Jonas Jost B. Jonas Jost B. Jonas Intravitreal panitumumab and retinal pigment epithelium proliferation in laser-induced retinal degeneration in rabbits Frontiers in Ophthalmology panitumumab epidermal growth factor receptor inhibitor macular degeneration retinal pigment epithelium macular scar intravitreal medication |
| title | Intravitreal panitumumab and retinal pigment epithelium proliferation in laser-induced retinal degeneration in rabbits |
| title_full | Intravitreal panitumumab and retinal pigment epithelium proliferation in laser-induced retinal degeneration in rabbits |
| title_fullStr | Intravitreal panitumumab and retinal pigment epithelium proliferation in laser-induced retinal degeneration in rabbits |
| title_full_unstemmed | Intravitreal panitumumab and retinal pigment epithelium proliferation in laser-induced retinal degeneration in rabbits |
| title_short | Intravitreal panitumumab and retinal pigment epithelium proliferation in laser-induced retinal degeneration in rabbits |
| title_sort | intravitreal panitumumab and retinal pigment epithelium proliferation in laser induced retinal degeneration in rabbits |
| topic | panitumumab epidermal growth factor receptor inhibitor macular degeneration retinal pigment epithelium macular scar intravitreal medication |
| url | https://www.frontiersin.org/articles/10.3389/fopht.2025.1641194/full |
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