Neurotrophins and Proneurotrophins as Biomarkers for Overactive Bladder Syndrome in Aging Females

<b>Background/Objectives</b>: Overactive bladder (OAB), common in elderly women, involves urgency, frequency, and nocturia, with complex phenotypes. The use of neurotrophins as non-invasive urinary biomarkers has been previously explored. The objective of this study was to assess the dia...

Full description

Saved in:
Bibliographic Details
Main Authors: Claudia Covarrubias, Philippe G. Cammisotto, Lysanne Campeau
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Metabolites
Subjects:
Online Access:https://www.mdpi.com/2218-1989/15/7/429
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1849419126555017216
author Claudia Covarrubias
Philippe G. Cammisotto
Lysanne Campeau
author_facet Claudia Covarrubias
Philippe G. Cammisotto
Lysanne Campeau
author_sort Claudia Covarrubias
collection DOAJ
description <b>Background/Objectives</b>: Overactive bladder (OAB), common in elderly women, involves urgency, frequency, and nocturia, with complex phenotypes. The use of neurotrophins as non-invasive urinary biomarkers has been previously explored. The objective of this study was to assess the diagnostic and therapeutic utility of urinary biomarkers in a Canadian population of aging female OAB patients. <b>Methods</b>: We conducted a single-center prospective study of aging female patients diagnosed with OAB and age-matched healthy controls, where we conducted pre- and post-treatment assessments using a combination of clinical questionnaires, voiding diaries, and urinary biomarkers nerve growth factor (NGF), proform of NGF (proNGF), brain-derived neurotrophic factor (BDNF), proform of BDNF (proBDNF), and neurotrophin receptor p75 extracellular domain (p75<sup>ECD</sup>)) quantified using ELISA. Baseline and post-treatment urinary biomarker levels in OAB patients were compared with those of controls. <b>Results</b>: OAB patients and controls at baseline displayed significant differences in neurotrophin levels and in their ratios of mature/precursors. In the post-treatment OAB cohort, only NGF and proNGF exhibited significant improvement correlating with clinical symptom relief. Biomarkers in non-responders remained unchanged, suggesting heterogeneity in therapeutic response. <b>Conclusions</b>: Urinary neurotrophins show promise as non-invasive diagnostic markers of OAB and monitoring treatment response in aging female patients. While this study focused on patients broadly diagnosed with OAB, future research should aim to classify OAB subtypes—such as those based on urodynamic studies or underlying pathophysiology—to better understand how urinary neurotrophins can differentiate between mechanisms like detrusor overactivity, detrusor underactivity, or bladder outlet obstruction. This will enhance their relevance in guiding personalized treatment strategies and predicting outcomes.
format Article
id doaj-art-67da54870355495b918d48adffdabd7f
institution Kabale University
issn 2218-1989
language English
publishDate 2025-06-01
publisher MDPI AG
record_format Article
series Metabolites
spelling doaj-art-67da54870355495b918d48adffdabd7f2025-08-20T03:32:14ZengMDPI AGMetabolites2218-19892025-06-0115742910.3390/metabo15070429Neurotrophins and Proneurotrophins as Biomarkers for Overactive Bladder Syndrome in Aging FemalesClaudia Covarrubias0Philippe G. Cammisotto1Lysanne Campeau2Lady Davis Institute, McGill University, Montreal, QC H3T1E2, CanadaLady Davis Institute, McGill University, Montreal, QC H3T1E2, CanadaLady Davis Institute, McGill University, Montreal, QC H3T1E2, Canada<b>Background/Objectives</b>: Overactive bladder (OAB), common in elderly women, involves urgency, frequency, and nocturia, with complex phenotypes. The use of neurotrophins as non-invasive urinary biomarkers has been previously explored. The objective of this study was to assess the diagnostic and therapeutic utility of urinary biomarkers in a Canadian population of aging female OAB patients. <b>Methods</b>: We conducted a single-center prospective study of aging female patients diagnosed with OAB and age-matched healthy controls, where we conducted pre- and post-treatment assessments using a combination of clinical questionnaires, voiding diaries, and urinary biomarkers nerve growth factor (NGF), proform of NGF (proNGF), brain-derived neurotrophic factor (BDNF), proform of BDNF (proBDNF), and neurotrophin receptor p75 extracellular domain (p75<sup>ECD</sup>)) quantified using ELISA. Baseline and post-treatment urinary biomarker levels in OAB patients were compared with those of controls. <b>Results</b>: OAB patients and controls at baseline displayed significant differences in neurotrophin levels and in their ratios of mature/precursors. In the post-treatment OAB cohort, only NGF and proNGF exhibited significant improvement correlating with clinical symptom relief. Biomarkers in non-responders remained unchanged, suggesting heterogeneity in therapeutic response. <b>Conclusions</b>: Urinary neurotrophins show promise as non-invasive diagnostic markers of OAB and monitoring treatment response in aging female patients. While this study focused on patients broadly diagnosed with OAB, future research should aim to classify OAB subtypes—such as those based on urodynamic studies or underlying pathophysiology—to better understand how urinary neurotrophins can differentiate between mechanisms like detrusor overactivity, detrusor underactivity, or bladder outlet obstruction. This will enhance their relevance in guiding personalized treatment strategies and predicting outcomes.https://www.mdpi.com/2218-1989/15/7/429overactive bladderbiomarkersneurotrophinsNGFBDNF
spellingShingle Claudia Covarrubias
Philippe G. Cammisotto
Lysanne Campeau
Neurotrophins and Proneurotrophins as Biomarkers for Overactive Bladder Syndrome in Aging Females
Metabolites
overactive bladder
biomarkers
neurotrophins
NGF
BDNF
title Neurotrophins and Proneurotrophins as Biomarkers for Overactive Bladder Syndrome in Aging Females
title_full Neurotrophins and Proneurotrophins as Biomarkers for Overactive Bladder Syndrome in Aging Females
title_fullStr Neurotrophins and Proneurotrophins as Biomarkers for Overactive Bladder Syndrome in Aging Females
title_full_unstemmed Neurotrophins and Proneurotrophins as Biomarkers for Overactive Bladder Syndrome in Aging Females
title_short Neurotrophins and Proneurotrophins as Biomarkers for Overactive Bladder Syndrome in Aging Females
title_sort neurotrophins and proneurotrophins as biomarkers for overactive bladder syndrome in aging females
topic overactive bladder
biomarkers
neurotrophins
NGF
BDNF
url https://www.mdpi.com/2218-1989/15/7/429
work_keys_str_mv AT claudiacovarrubias neurotrophinsandproneurotrophinsasbiomarkersforoveractivebladdersyndromeinagingfemales
AT philippegcammisotto neurotrophinsandproneurotrophinsasbiomarkersforoveractivebladdersyndromeinagingfemales
AT lysannecampeau neurotrophinsandproneurotrophinsasbiomarkersforoveractivebladdersyndromeinagingfemales