Transcriptome profiling of intrahepatocytic Plasmodium and their host hepatocytes based on the infection phase and the zonation of the liver
Intrahepatocytic development is a key stage in human Plasmodium infection, in which sporozoites replicate and transform into merozoites. Due to technological limitations, however, previous gene expression studies on malaria parasite liver infection were mostly conducted in vitro. In order to bridge...
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Frontiers Media S.A.
2025-04-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2025.1548487/full |
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| author | Zhuning Mo Zhuning Mo Yuhong Chen Yuhong Chen Yujuan Qin Yujuan Qin Jian Song Jian Song Jian Song |
| author_facet | Zhuning Mo Zhuning Mo Yuhong Chen Yuhong Chen Yujuan Qin Yujuan Qin Jian Song Jian Song Jian Song |
| author_sort | Zhuning Mo |
| collection | DOAJ |
| description | Intrahepatocytic development is a key stage in human Plasmodium infection, in which sporozoites replicate and transform into merozoites. Due to technological limitations, however, previous gene expression studies on malaria parasite liver infection were mostly conducted in vitro. In order to bridge these gaps, our current study compared the gene expression of in vitro-infected parasites at different time points with that of in vivo-infected parasites and revealed distinct patterns between parasite subpopulations in vitro and in vivo. A joint investigation of the Plasmodium transcriptome and their host transcriptome was carried out to confer a comprehensive analysis of gene expression in the liver stage of Plasmodium infection in vivo, which is similar to the disease setting, and therefore deepen our understanding of parasite and host transcriptional dynamics during intrahepatocytic infection. |
| format | Article |
| id | doaj-art-67d5da0ce7fb45fb9a1bcd6415b6cb49 |
| institution | OA Journals |
| issn | 1664-8021 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj-art-67d5da0ce7fb45fb9a1bcd6415b6cb492025-08-20T02:27:45ZengFrontiers Media S.A.Frontiers in Genetics1664-80212025-04-011610.3389/fgene.2025.15484871548487Transcriptome profiling of intrahepatocytic Plasmodium and their host hepatocytes based on the infection phase and the zonation of the liverZhuning Mo0Zhuning Mo1Yuhong Chen2Yuhong Chen3Yujuan Qin4Yujuan Qin5Jian Song6Jian Song7Jian Song8Institute of Cardiovascular Sciences, Guangxi Academy of Medical Sciences, Nanning, ChinaDepartment of Blood Transfusion, The People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, ChinaInstitute of Cardiovascular Sciences, Guangxi Academy of Medical Sciences, Nanning, ChinaDepartment of Blood Transfusion, The People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, ChinaInstitute of Cardiovascular Sciences, Guangxi Academy of Medical Sciences, Nanning, ChinaDepartment of Blood Transfusion, The People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, ChinaInstitute of Cardiovascular Sciences, Guangxi Academy of Medical Sciences, Nanning, ChinaDepartment of Blood Transfusion, The People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, ChinaDepartment of Radiation Oncology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaIntrahepatocytic development is a key stage in human Plasmodium infection, in which sporozoites replicate and transform into merozoites. Due to technological limitations, however, previous gene expression studies on malaria parasite liver infection were mostly conducted in vitro. In order to bridge these gaps, our current study compared the gene expression of in vitro-infected parasites at different time points with that of in vivo-infected parasites and revealed distinct patterns between parasite subpopulations in vitro and in vivo. A joint investigation of the Plasmodium transcriptome and their host transcriptome was carried out to confer a comprehensive analysis of gene expression in the liver stage of Plasmodium infection in vivo, which is similar to the disease setting, and therefore deepen our understanding of parasite and host transcriptional dynamics during intrahepatocytic infection.https://www.frontiersin.org/articles/10.3389/fgene.2025.1548487/fullPlasmodiumintraerythrocytic stagedual transcriptomespatiotemporal analysescontrast between in vivo and in vitro |
| spellingShingle | Zhuning Mo Zhuning Mo Yuhong Chen Yuhong Chen Yujuan Qin Yujuan Qin Jian Song Jian Song Jian Song Transcriptome profiling of intrahepatocytic Plasmodium and their host hepatocytes based on the infection phase and the zonation of the liver Frontiers in Genetics Plasmodium intraerythrocytic stage dual transcriptome spatiotemporal analyses contrast between in vivo and in vitro |
| title | Transcriptome profiling of intrahepatocytic Plasmodium and their host hepatocytes based on the infection phase and the zonation of the liver |
| title_full | Transcriptome profiling of intrahepatocytic Plasmodium and their host hepatocytes based on the infection phase and the zonation of the liver |
| title_fullStr | Transcriptome profiling of intrahepatocytic Plasmodium and their host hepatocytes based on the infection phase and the zonation of the liver |
| title_full_unstemmed | Transcriptome profiling of intrahepatocytic Plasmodium and their host hepatocytes based on the infection phase and the zonation of the liver |
| title_short | Transcriptome profiling of intrahepatocytic Plasmodium and their host hepatocytes based on the infection phase and the zonation of the liver |
| title_sort | transcriptome profiling of intrahepatocytic plasmodium and their host hepatocytes based on the infection phase and the zonation of the liver |
| topic | Plasmodium intraerythrocytic stage dual transcriptome spatiotemporal analyses contrast between in vivo and in vitro |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2025.1548487/full |
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