Gustatory sensitivity to amino acids in bumblebee mouthparts

Bees rely on amino acids from nectar and pollen for essential physiological functions. While nectar typically contains low (less than 1 mM) amino acid concentrations, levels in pollen are higher but variable (10–200 mM). Behavioural studies suggest bumblebees have preferences for specific amino acid...

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Main Authors: Sergio Rossoni, Rachel H. Parkinson, Jeremy E. Niven, Elizabeth Nicholls
Format: Article
Language:English
Published: The Royal Society 2025-05-01
Series:Royal Society Open Science
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Online Access:https://royalsocietypublishing.org/doi/10.1098/rsos.250465
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author Sergio Rossoni
Rachel H. Parkinson
Jeremy E. Niven
Elizabeth Nicholls
author_facet Sergio Rossoni
Rachel H. Parkinson
Jeremy E. Niven
Elizabeth Nicholls
author_sort Sergio Rossoni
collection DOAJ
description Bees rely on amino acids from nectar and pollen for essential physiological functions. While nectar typically contains low (less than 1 mM) amino acid concentrations, levels in pollen are higher but variable (10–200 mM). Behavioural studies suggest bumblebees have preferences for specific amino acids but whether such preferences are mediated via gustatory mechanisms remains unclear. This study explores bumblebees’ (Bombus terrestris) gustatory sensitivity to two essential amino acids found in nectar and pollen, valine and lysine, using electrophysiological recordings from gustatory sensilla on their mouthparts. Valine elicited a concentration-dependent response from 0.1 mM, indicating that bumblebees could perceive valine at concentrations found naturally in nectar and pollen. By contrast, lysine failed to evoke a response across tested concentrations (0.1–500 mM). The absence of lysine detection raises questions about the specificity and diversity of amino acid-sensitive receptors in bumblebees. Bees responded to valine at lower concentrations than sucrose, suggesting comparatively higher sensitivity (EC50: 0.7 mM versus 3.91 mM for sucrose). Our findings indicate that bumblebees can evaluate the amino acid content of pollen and nectar using pre-ingestive cues, rather than relying on post-ingestive cues or feedback from their nestmates. Such sensory capabilities probably impact foraging strategies, with implications for plant–bee interactions and pollination.
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spelling doaj-art-67d10e58d50d4b8fab5b7084dffd61792025-08-20T03:44:52ZengThe Royal SocietyRoyal Society Open Science2054-57032025-05-0112510.1098/rsos.250465Gustatory sensitivity to amino acids in bumblebee mouthpartsSergio Rossoni0Rachel H. Parkinson1Jeremy E. Niven2Elizabeth Nicholls3Department of Ecology and Evolution, University of Sussex , Brighton, East Sussex, UKDepartment of Biology, University of Oxford , Oxford, Oxfordshire, UKDepartment of Ecology and Evolution, University of Sussex , Brighton, East Sussex, UKDepartment of Ecology and Evolution, University of Sussex , Brighton, East Sussex, UKBees rely on amino acids from nectar and pollen for essential physiological functions. While nectar typically contains low (less than 1 mM) amino acid concentrations, levels in pollen are higher but variable (10–200 mM). Behavioural studies suggest bumblebees have preferences for specific amino acids but whether such preferences are mediated via gustatory mechanisms remains unclear. This study explores bumblebees’ (Bombus terrestris) gustatory sensitivity to two essential amino acids found in nectar and pollen, valine and lysine, using electrophysiological recordings from gustatory sensilla on their mouthparts. Valine elicited a concentration-dependent response from 0.1 mM, indicating that bumblebees could perceive valine at concentrations found naturally in nectar and pollen. By contrast, lysine failed to evoke a response across tested concentrations (0.1–500 mM). The absence of lysine detection raises questions about the specificity and diversity of amino acid-sensitive receptors in bumblebees. Bees responded to valine at lower concentrations than sucrose, suggesting comparatively higher sensitivity (EC50: 0.7 mM versus 3.91 mM for sucrose). Our findings indicate that bumblebees can evaluate the amino acid content of pollen and nectar using pre-ingestive cues, rather than relying on post-ingestive cues or feedback from their nestmates. Such sensory capabilities probably impact foraging strategies, with implications for plant–bee interactions and pollination.https://royalsocietypublishing.org/doi/10.1098/rsos.250465tastevalinelysineproteinpollennectar
spellingShingle Sergio Rossoni
Rachel H. Parkinson
Jeremy E. Niven
Elizabeth Nicholls
Gustatory sensitivity to amino acids in bumblebee mouthparts
Royal Society Open Science
taste
valine
lysine
protein
pollen
nectar
title Gustatory sensitivity to amino acids in bumblebee mouthparts
title_full Gustatory sensitivity to amino acids in bumblebee mouthparts
title_fullStr Gustatory sensitivity to amino acids in bumblebee mouthparts
title_full_unstemmed Gustatory sensitivity to amino acids in bumblebee mouthparts
title_short Gustatory sensitivity to amino acids in bumblebee mouthparts
title_sort gustatory sensitivity to amino acids in bumblebee mouthparts
topic taste
valine
lysine
protein
pollen
nectar
url https://royalsocietypublishing.org/doi/10.1098/rsos.250465
work_keys_str_mv AT sergiorossoni gustatorysensitivitytoaminoacidsinbumblebeemouthparts
AT rachelhparkinson gustatorysensitivitytoaminoacidsinbumblebeemouthparts
AT jeremyeniven gustatorysensitivitytoaminoacidsinbumblebeemouthparts
AT elizabethnicholls gustatorysensitivitytoaminoacidsinbumblebeemouthparts