The Protective Effects of Maresin 1 in the OVA-Induced Asthma Mouse Model
Asthma is a chronic inflammatory disease that cannot be cured. Maresin 1 (MaR1) is a specific lipid synthesized by macrophages that exhibits powerful anti-inflammatory effects in various inflammatory diseases. The goal of this study was to evaluate the effect of MaR1 on allergic asthma using an oval...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2021/4131420 |
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| author | Guochun Ou Qin Liu Chengxiu Yu Xiaoju Chen Wenbo Zhang Yong Chen Tao Wang Yongqiang Luo Guolu Jiang Mingmei Zhu Hongmei Li Mei Zeng |
| author_facet | Guochun Ou Qin Liu Chengxiu Yu Xiaoju Chen Wenbo Zhang Yong Chen Tao Wang Yongqiang Luo Guolu Jiang Mingmei Zhu Hongmei Li Mei Zeng |
| author_sort | Guochun Ou |
| collection | DOAJ |
| description | Asthma is a chronic inflammatory disease that cannot be cured. Maresin 1 (MaR1) is a specific lipid synthesized by macrophages that exhibits powerful anti-inflammatory effects in various inflammatory diseases. The goal of this study was to evaluate the effect of MaR1 on allergic asthma using an ovalbumin- (OVA-) induced asthma model. Thirty BALB/c mice were randomly allocated to control, OVA, and MaR1 + OVA groups. Mice were sacrificed 24 hours after the end of the last challenge, and serum, bronchoalveolar lavage fluid (BALF), and lung tissue were collected for further analysis. Western blotting was used to measure the protein level of IκBα, the activation of the NF-κB signaling pathway, and the expression of NF-κB downstream inflammatory cytokines. Quantitative real-time polymerase chain reactions (qRT-PCRs) were used to evaluate the expression levels of COX-2 and ICAM-1 in lung tissues. We found that high doses of MaR1 were most effective in preventing OVA-induced inflammatory cell infiltration and excessive mucus production in lung tissue, reducing the number of inflammatory cells in the BALF and inhibiting the expression of serum or BALF-associated inflammatory factors. Furthermore, high-dose MaR1 treatment markedly suppressed the activation of the NF-κB signaling pathway, the degradation of IκBα, and the expression of inflammatory genes downstream of NF-κB, such as COX-2 and ICAM-1, in the OVA-induced asthma mouse model. Our findings indicate that MaR1 may play a critical role in OVA-induced asthma and may be therapeutically useful for the management of asthma. |
| format | Article |
| id | doaj-art-67cb8734d8ad4938a177b92e682cc610 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-67cb8734d8ad4938a177b92e682cc6102025-02-03T01:24:39ZengWileyMediators of Inflammation0962-93511466-18612021-01-01202110.1155/2021/41314204131420The Protective Effects of Maresin 1 in the OVA-Induced Asthma Mouse ModelGuochun Ou0Qin Liu1Chengxiu Yu2Xiaoju Chen3Wenbo Zhang4Yong Chen5Tao Wang6Yongqiang Luo7Guolu Jiang8Mingmei Zhu9Hongmei Li10Mei Zeng11Department of Respiratory and Critical Care Medicine, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, ChinaDepartment of Respiratory and Critical Care Medicine, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, ChinaDepartment of Respiratory and Critical Care Medicine, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, ChinaDepartment of Respiratory and Critical Care Medicine, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, ChinaDepartment of Respiratory and Critical Care Medicine, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, ChinaDepartment of Respiratory and Critical Care Medicine, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, ChinaDepartment of Respiratory and Critical Care Medicine, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, ChinaDepartment of Gastroenterology, Anyue County People’s Hospital, Ziyang, Sichuan 642350, ChinaDepartment of Respiratory and Critical Care Medicine, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, ChinaDepartment of Respiratory and Critical Care Medicine, Suining Central Hospital, Suining, Sichuan 629000, ChinaDepartment of Transfusion, Suining Central Hospital, Suining, Sichuan 629000, ChinaInstitute of Rheumatology and Immunology, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, ChinaAsthma is a chronic inflammatory disease that cannot be cured. Maresin 1 (MaR1) is a specific lipid synthesized by macrophages that exhibits powerful anti-inflammatory effects in various inflammatory diseases. The goal of this study was to evaluate the effect of MaR1 on allergic asthma using an ovalbumin- (OVA-) induced asthma model. Thirty BALB/c mice were randomly allocated to control, OVA, and MaR1 + OVA groups. Mice were sacrificed 24 hours after the end of the last challenge, and serum, bronchoalveolar lavage fluid (BALF), and lung tissue were collected for further analysis. Western blotting was used to measure the protein level of IκBα, the activation of the NF-κB signaling pathway, and the expression of NF-κB downstream inflammatory cytokines. Quantitative real-time polymerase chain reactions (qRT-PCRs) were used to evaluate the expression levels of COX-2 and ICAM-1 in lung tissues. We found that high doses of MaR1 were most effective in preventing OVA-induced inflammatory cell infiltration and excessive mucus production in lung tissue, reducing the number of inflammatory cells in the BALF and inhibiting the expression of serum or BALF-associated inflammatory factors. Furthermore, high-dose MaR1 treatment markedly suppressed the activation of the NF-κB signaling pathway, the degradation of IκBα, and the expression of inflammatory genes downstream of NF-κB, such as COX-2 and ICAM-1, in the OVA-induced asthma mouse model. Our findings indicate that MaR1 may play a critical role in OVA-induced asthma and may be therapeutically useful for the management of asthma.http://dx.doi.org/10.1155/2021/4131420 |
| spellingShingle | Guochun Ou Qin Liu Chengxiu Yu Xiaoju Chen Wenbo Zhang Yong Chen Tao Wang Yongqiang Luo Guolu Jiang Mingmei Zhu Hongmei Li Mei Zeng The Protective Effects of Maresin 1 in the OVA-Induced Asthma Mouse Model Mediators of Inflammation |
| title | The Protective Effects of Maresin 1 in the OVA-Induced Asthma Mouse Model |
| title_full | The Protective Effects of Maresin 1 in the OVA-Induced Asthma Mouse Model |
| title_fullStr | The Protective Effects of Maresin 1 in the OVA-Induced Asthma Mouse Model |
| title_full_unstemmed | The Protective Effects of Maresin 1 in the OVA-Induced Asthma Mouse Model |
| title_short | The Protective Effects of Maresin 1 in the OVA-Induced Asthma Mouse Model |
| title_sort | protective effects of maresin 1 in the ova induced asthma mouse model |
| url | http://dx.doi.org/10.1155/2021/4131420 |
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