Establishment and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Detection of Raccoon Dog in Meat Mixtures

Raccoon dog (Nyctereutes procyonoides) is an economically important animal used for fur production, but consuming its meat is injurious to human health. Currently, no rapid and sensitive method for detecting raccoon dog meat in meat mixtures is available. In this study, we developed an easily applic...

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Main Authors: Jinhua Liu, Yanyu Shi, Siying Teng, Lianpeng Wu, Xinmin Zhang
Format: Article
Language:English
Published: Wiley 2017-01-01
Series:Journal of Food Quality
Online Access:http://dx.doi.org/10.1155/2017/9319035
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author Jinhua Liu
Yanyu Shi
Siying Teng
Lianpeng Wu
Xinmin Zhang
author_facet Jinhua Liu
Yanyu Shi
Siying Teng
Lianpeng Wu
Xinmin Zhang
author_sort Jinhua Liu
collection DOAJ
description Raccoon dog (Nyctereutes procyonoides) is an economically important animal used for fur production, but consuming its meat is injurious to human health. Currently, no rapid and sensitive method for detecting raccoon dog meat in meat mixtures is available. In this study, we developed an easily applicable, rapid, and economically feasible method for identifying the presence of raccoon dog in meat mixtures based on loop-mediated isothermal amplification (LAMP). Four sets of LAMP primers were tested at different temperatures, and the primers that worked best at 62°C (set 2) were determined. In the LAMP assay, there was no cross-reactivity with the meat procured from other species of animals and the detection limit of DNA concentration was 0.1 pg·μL−1, slightly higher than TaqMan real-time PCR (0.01 pg·μL−1), but sensitivity of 0.1 pg·μL−1 complies with most requirements of routine analysis. Moreover, by the LAMP method, the meat mixtures containing more than 0.5% of the raccoon dog component were directly detected (without DNA extraction) in the supernatant isolated from the meat mixtures after performing repeated cycles of thawing and freezing of minced meat mixtures. Our results show that LAMP assay is a valuable, straightforward, and sensitive detection tool for identification of raccoon dog meat in mixtures.
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institution Kabale University
issn 0146-9428
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publishDate 2017-01-01
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spelling doaj-art-67ca6d5e40444a619ee5e0d96bc8cef92025-08-20T03:34:01ZengWileyJournal of Food Quality0146-94281745-45572017-01-01201710.1155/2017/93190359319035Establishment and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Detection of Raccoon Dog in Meat MixturesJinhua Liu0Yanyu Shi1Siying Teng2Lianpeng Wu3Xinmin Zhang4School of Pharmaceutical Sciences, Jilin University, Changchun, Jilin, ChinaJilin Product Quality Supervision Inspection, Changchun, Jilin, ChinaDepartment of Ophthalmology, First Hospital of Jilin University, Changchun, Jilin, ChinaJilin Entry-Exit Inspection and Quarantine Bureau, Changchun, Jilin, ChinaSchool of Pharmaceutical Sciences, Jilin University, Changchun, Jilin, ChinaRaccoon dog (Nyctereutes procyonoides) is an economically important animal used for fur production, but consuming its meat is injurious to human health. Currently, no rapid and sensitive method for detecting raccoon dog meat in meat mixtures is available. In this study, we developed an easily applicable, rapid, and economically feasible method for identifying the presence of raccoon dog in meat mixtures based on loop-mediated isothermal amplification (LAMP). Four sets of LAMP primers were tested at different temperatures, and the primers that worked best at 62°C (set 2) were determined. In the LAMP assay, there was no cross-reactivity with the meat procured from other species of animals and the detection limit of DNA concentration was 0.1 pg·μL−1, slightly higher than TaqMan real-time PCR (0.01 pg·μL−1), but sensitivity of 0.1 pg·μL−1 complies with most requirements of routine analysis. Moreover, by the LAMP method, the meat mixtures containing more than 0.5% of the raccoon dog component were directly detected (without DNA extraction) in the supernatant isolated from the meat mixtures after performing repeated cycles of thawing and freezing of minced meat mixtures. Our results show that LAMP assay is a valuable, straightforward, and sensitive detection tool for identification of raccoon dog meat in mixtures.http://dx.doi.org/10.1155/2017/9319035
spellingShingle Jinhua Liu
Yanyu Shi
Siying Teng
Lianpeng Wu
Xinmin Zhang
Establishment and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Detection of Raccoon Dog in Meat Mixtures
Journal of Food Quality
title Establishment and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Detection of Raccoon Dog in Meat Mixtures
title_full Establishment and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Detection of Raccoon Dog in Meat Mixtures
title_fullStr Establishment and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Detection of Raccoon Dog in Meat Mixtures
title_full_unstemmed Establishment and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Detection of Raccoon Dog in Meat Mixtures
title_short Establishment and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Detection of Raccoon Dog in Meat Mixtures
title_sort establishment and evaluation of a loop mediated isothermal amplification assay for detection of raccoon dog in meat mixtures
url http://dx.doi.org/10.1155/2017/9319035
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