Comparative antioxidant and antimicrobial activities of Nigella sativa flower, leaf, stem, and seed derived silver nanoparticles
This study was aimed to synthesize biogenic silver nanoparticles (AgNPs) using Nigella sativa (N. sativa) plant extracts as bio-reductant. The fabricated nanoparticles were characterized by UV–visible, FTIR, and XRD. The extracts and AgNPs were then evaluated for their antioxidant and antimicrobial...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-10-01
|
| Series: | Results in Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211715624005046 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850275423623053312 |
|---|---|
| author | Fizza Perveen Fakhria A. Al-Joufi Rabia Zeb Naila Qamar Abdul Jabbar Muhammad Naveed Umar Muhammad Zahoor |
| author_facet | Fizza Perveen Fakhria A. Al-Joufi Rabia Zeb Naila Qamar Abdul Jabbar Muhammad Naveed Umar Muhammad Zahoor |
| author_sort | Fizza Perveen |
| collection | DOAJ |
| description | This study was aimed to synthesize biogenic silver nanoparticles (AgNPs) using Nigella sativa (N. sativa) plant extracts as bio-reductant. The fabricated nanoparticles were characterized by UV–visible, FTIR, and XRD. The extracts and AgNPs were then evaluated for their antioxidant and antimicrobial potentials using DPPH (2, 2-diphenyl-1-picrylhydrazyl) assay and agar well diffusion assay correspondingly. The color change from pale yellow to tan brownish confirmed the fabrication of nanoparticles. The broad peak from 420 to 430 in the UV–Visible spectrum also confirmed AgNPs formation. In XRD spectrum the peaks indices (111), (200), (220), and (311) belonging to 2θ values of 38.101, 44.370, 64.179, and 77.549 indicated the presence of silver crystals (JCPDS file no. 00-001-1167). The presence of organic functional groups in the FTIR spectra confirmed the involvement of plant phytochemicals in fabrication of nanoparticles. Comparatively strong antioxidant potential was recorded for silver nanoparticles in comparison to parental extract. The lowest IC50 recorded was in the case flower based AgNPs was 70 µl where it corresponding crude extract resulted in IC50 of 910 µl. Highest IC50 was recorded for stem extract based NPs (100 µl). The antifungal potential of AgNPs from flower was high (24 mm) and that of the stem extract-based NPs was low (19 mm) whereas the antibacterial potential of the stem and seed extract-based extract was comparable however, the leaf and flower-based extract-based NPs were also, substantial. It was concluded from the results that all the whole Nigella sativa plant contained the reductant phytochemicals which be effectively used to fabricate NPs of the desired sizes. The fabricated NPs also exhibited good antioxidant and antimicrobial properties and can be considered as alternative drugs subjected to further verifications of the results in animal model. |
| format | Article |
| id | doaj-art-67c8baf7b1df434f91d1a4ab3d8f4c85 |
| institution | OA Journals |
| issn | 2211-7156 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Chemistry |
| spelling | doaj-art-67c8baf7b1df434f91d1a4ab3d8f4c852025-08-20T01:50:45ZengElsevierResults in Chemistry2211-71562024-10-011110180810.1016/j.rechem.2024.101808Comparative antioxidant and antimicrobial activities of Nigella sativa flower, leaf, stem, and seed derived silver nanoparticlesFizza Perveen0Fakhria A. Al-Joufi1Rabia Zeb2Naila Qamar3Abdul Jabbar4Muhammad Naveed Umar5Muhammad Zahoor6Department of Medical Lab Technology, The University of Haripur, 22620 Khyber, Pakhtunkhwa, PakistanDepartment of Pharmacology, College of Pharmacy, Jouf University, 72341 Aljouf, Saudi ArabiaDepartment of Medical Lab Technology, The University of Haripur, 22620 Khyber, Pakhtunkhwa, PakistanDepartment of Medical Lab Technology, The University of Haripur, 22620 Khyber, Pakhtunkhwa, PakistanDepartment of Medical Lab Technology, The University of Haripur, 22620 Khyber, Pakhtunkhwa, PakistanDepartment of Chemistry, University of Malakand, Chakdara Dir Lower, KPK, 18800, PakistanDepartment of Biochemistry, University of Malakand, Chakdara Dir Lower, KPK, 18800, Pakistan; Corresponding author.This study was aimed to synthesize biogenic silver nanoparticles (AgNPs) using Nigella sativa (N. sativa) plant extracts as bio-reductant. The fabricated nanoparticles were characterized by UV–visible, FTIR, and XRD. The extracts and AgNPs were then evaluated for their antioxidant and antimicrobial potentials using DPPH (2, 2-diphenyl-1-picrylhydrazyl) assay and agar well diffusion assay correspondingly. The color change from pale yellow to tan brownish confirmed the fabrication of nanoparticles. The broad peak from 420 to 430 in the UV–Visible spectrum also confirmed AgNPs formation. In XRD spectrum the peaks indices (111), (200), (220), and (311) belonging to 2θ values of 38.101, 44.370, 64.179, and 77.549 indicated the presence of silver crystals (JCPDS file no. 00-001-1167). The presence of organic functional groups in the FTIR spectra confirmed the involvement of plant phytochemicals in fabrication of nanoparticles. Comparatively strong antioxidant potential was recorded for silver nanoparticles in comparison to parental extract. The lowest IC50 recorded was in the case flower based AgNPs was 70 µl where it corresponding crude extract resulted in IC50 of 910 µl. Highest IC50 was recorded for stem extract based NPs (100 µl). The antifungal potential of AgNPs from flower was high (24 mm) and that of the stem extract-based NPs was low (19 mm) whereas the antibacterial potential of the stem and seed extract-based extract was comparable however, the leaf and flower-based extract-based NPs were also, substantial. It was concluded from the results that all the whole Nigella sativa plant contained the reductant phytochemicals which be effectively used to fabricate NPs of the desired sizes. The fabricated NPs also exhibited good antioxidant and antimicrobial properties and can be considered as alternative drugs subjected to further verifications of the results in animal model.http://www.sciencedirect.com/science/article/pii/S2211715624005046Silver nanoparticlesNigella sativaAntioxidantAntimicrobial activityNanotechnology |
| spellingShingle | Fizza Perveen Fakhria A. Al-Joufi Rabia Zeb Naila Qamar Abdul Jabbar Muhammad Naveed Umar Muhammad Zahoor Comparative antioxidant and antimicrobial activities of Nigella sativa flower, leaf, stem, and seed derived silver nanoparticles Results in Chemistry Silver nanoparticles Nigella sativa Antioxidant Antimicrobial activity Nanotechnology |
| title | Comparative antioxidant and antimicrobial activities of Nigella sativa flower, leaf, stem, and seed derived silver nanoparticles |
| title_full | Comparative antioxidant and antimicrobial activities of Nigella sativa flower, leaf, stem, and seed derived silver nanoparticles |
| title_fullStr | Comparative antioxidant and antimicrobial activities of Nigella sativa flower, leaf, stem, and seed derived silver nanoparticles |
| title_full_unstemmed | Comparative antioxidant and antimicrobial activities of Nigella sativa flower, leaf, stem, and seed derived silver nanoparticles |
| title_short | Comparative antioxidant and antimicrobial activities of Nigella sativa flower, leaf, stem, and seed derived silver nanoparticles |
| title_sort | comparative antioxidant and antimicrobial activities of nigella sativa flower leaf stem and seed derived silver nanoparticles |
| topic | Silver nanoparticles Nigella sativa Antioxidant Antimicrobial activity Nanotechnology |
| url | http://www.sciencedirect.com/science/article/pii/S2211715624005046 |
| work_keys_str_mv | AT fizzaperveen comparativeantioxidantandantimicrobialactivitiesofnigellasativaflowerleafstemandseedderivedsilvernanoparticles AT fakhriaaaljoufi comparativeantioxidantandantimicrobialactivitiesofnigellasativaflowerleafstemandseedderivedsilvernanoparticles AT rabiazeb comparativeantioxidantandantimicrobialactivitiesofnigellasativaflowerleafstemandseedderivedsilvernanoparticles AT nailaqamar comparativeantioxidantandantimicrobialactivitiesofnigellasativaflowerleafstemandseedderivedsilvernanoparticles AT abduljabbar comparativeantioxidantandantimicrobialactivitiesofnigellasativaflowerleafstemandseedderivedsilvernanoparticles AT muhammadnaveedumar comparativeantioxidantandantimicrobialactivitiesofnigellasativaflowerleafstemandseedderivedsilvernanoparticles AT muhammadzahoor comparativeantioxidantandantimicrobialactivitiesofnigellasativaflowerleafstemandseedderivedsilvernanoparticles |