CRMP4 and CRMP2 Interact to Coordinate Cytoskeleton Dynamics, Regulating Growth Cone Development and Axon Elongation
Cytoskeleton dynamics are critical phenomena that underpin many fundamental cellular processes. Collapsin response mediator proteins (CRMPs) are highly expressed in the developing nervous system, mediating growth cone guidance, neuronal polarity, and axonal elongation. However, whether and how CRMPs...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2015/947423 |
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| author | Minghui Tan Caihui Cha Yongheng Ye Jifeng Zhang Sumei Li Fengming Wu Sitang Gong Guoqing Guo |
| author_facet | Minghui Tan Caihui Cha Yongheng Ye Jifeng Zhang Sumei Li Fengming Wu Sitang Gong Guoqing Guo |
| author_sort | Minghui Tan |
| collection | DOAJ |
| description | Cytoskeleton dynamics are critical phenomena that underpin many fundamental cellular processes. Collapsin response mediator proteins (CRMPs) are highly expressed in the developing nervous system, mediating growth cone guidance, neuronal polarity, and axonal elongation. However, whether and how CRMPs associate with microtubules and actin coordinated cytoskeletal dynamics remain unknown. In this study, we demonstrated that CRMP2 and CRMP4 interacted with tubulin and actin in vitro and colocalized with the cytoskeleton in the transition-zone in developing growth cones. CRMP2 and CRMP4 also interacted with one another coordinately to promote growth cone development and axonal elongation. Genetic silencing of CRMP2 enhanced, whereas overexpression of CRMP2 suppressed, the inhibitory effects of CRMP4 knockdown on axonal development. In addition, knockdown of CRMP2 or overexpression of truncated CRMP2 reversed the promoting effect of CRMP4. With the overexpression of truncated CRMP2 or CRMP4 lacking the cytoskeleton interaction domain, the promoting effect of CRMP was suppressed. These data suggest a model in which CRMP2 and CRMP4 form complexes to bridge microtubules and actin and thus work cooperatively to regulate growth cone development and axonal elongation. |
| format | Article |
| id | doaj-art-67a7f1e42b934af8b7440a0009b49b2d |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-67a7f1e42b934af8b7440a0009b49b2d2025-02-03T01:20:33ZengWileyNeural Plasticity2090-59041687-54432015-01-01201510.1155/2015/947423947423CRMP4 and CRMP2 Interact to Coordinate Cytoskeleton Dynamics, Regulating Growth Cone Development and Axon ElongationMinghui Tan0Caihui Cha1Yongheng Ye2Jifeng Zhang3Sumei Li4Fengming Wu5Sitang Gong6Guoqing Guo7Department of Anatomy, Medical College of Jinan University, Guangzhou 510630, ChinaDepartment of Pediatrics, The First Affiliated Hospital of Jinan University, Guangzhou 510630, ChinaDepartment of Anatomy, Medical College of Jinan University, Guangzhou 510630, ChinaDepartment of Anatomy, Medical College of Jinan University, Guangzhou 510630, ChinaDepartment of Anatomy, Medical College of Jinan University, Guangzhou 510630, ChinaDepartment of Anatomy, Medical College of Jinan University, Guangzhou 510630, ChinaDepartment of Pediatrics, The First Affiliated Hospital of Jinan University, Guangzhou 510630, ChinaDepartment of Anatomy, Medical College of Jinan University, Guangzhou 510630, ChinaCytoskeleton dynamics are critical phenomena that underpin many fundamental cellular processes. Collapsin response mediator proteins (CRMPs) are highly expressed in the developing nervous system, mediating growth cone guidance, neuronal polarity, and axonal elongation. However, whether and how CRMPs associate with microtubules and actin coordinated cytoskeletal dynamics remain unknown. In this study, we demonstrated that CRMP2 and CRMP4 interacted with tubulin and actin in vitro and colocalized with the cytoskeleton in the transition-zone in developing growth cones. CRMP2 and CRMP4 also interacted with one another coordinately to promote growth cone development and axonal elongation. Genetic silencing of CRMP2 enhanced, whereas overexpression of CRMP2 suppressed, the inhibitory effects of CRMP4 knockdown on axonal development. In addition, knockdown of CRMP2 or overexpression of truncated CRMP2 reversed the promoting effect of CRMP4. With the overexpression of truncated CRMP2 or CRMP4 lacking the cytoskeleton interaction domain, the promoting effect of CRMP was suppressed. These data suggest a model in which CRMP2 and CRMP4 form complexes to bridge microtubules and actin and thus work cooperatively to regulate growth cone development and axonal elongation.http://dx.doi.org/10.1155/2015/947423 |
| spellingShingle | Minghui Tan Caihui Cha Yongheng Ye Jifeng Zhang Sumei Li Fengming Wu Sitang Gong Guoqing Guo CRMP4 and CRMP2 Interact to Coordinate Cytoskeleton Dynamics, Regulating Growth Cone Development and Axon Elongation Neural Plasticity |
| title | CRMP4 and CRMP2 Interact to Coordinate Cytoskeleton Dynamics, Regulating Growth Cone Development and Axon Elongation |
| title_full | CRMP4 and CRMP2 Interact to Coordinate Cytoskeleton Dynamics, Regulating Growth Cone Development and Axon Elongation |
| title_fullStr | CRMP4 and CRMP2 Interact to Coordinate Cytoskeleton Dynamics, Regulating Growth Cone Development and Axon Elongation |
| title_full_unstemmed | CRMP4 and CRMP2 Interact to Coordinate Cytoskeleton Dynamics, Regulating Growth Cone Development and Axon Elongation |
| title_short | CRMP4 and CRMP2 Interact to Coordinate Cytoskeleton Dynamics, Regulating Growth Cone Development and Axon Elongation |
| title_sort | crmp4 and crmp2 interact to coordinate cytoskeleton dynamics regulating growth cone development and axon elongation |
| url | http://dx.doi.org/10.1155/2015/947423 |
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