Study of Ultra-weak CW and Amplitude-Modulated Microwaves effects on stem Cell Proliferation: an Experimental and Hypothetical Approach

Weak microwave radiation (WMR) in our environment has raised health concerns in the public. Among those, communication frequencies are more than ever becoming widespread and their effects need thorough studies. A correct understanding of these effects in-vivo by in-vitro experiments shall preferenti...

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Main Authors: Yashar Rouzbahani, Mousa Kehtari, Mahdi Imani, Mehrdad Saviz, Iman Shabani, Farzad Moradikhah, Farhad Alizadeh, Mohammad-Hossein Karami, Ehsan Seyedjafari, Najmeh Jooyan, Zahra Mostajabi, Reza Faraji-Dana
Format: Article
Language:English
Published: Amirkabir University of Technology 2020-06-01
Series:AUT Journal of Electrical Engineering
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Online Access:https://eej.aut.ac.ir/article_3860_c17e42da17ed5b005b245ea8d617b512.pdf
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author Yashar Rouzbahani
Mousa Kehtari
Mahdi Imani
Mehrdad Saviz
Iman Shabani
Farzad Moradikhah
Farhad Alizadeh
Mohammad-Hossein Karami
Ehsan Seyedjafari
Najmeh Jooyan
Zahra Mostajabi
Reza Faraji-Dana
author_facet Yashar Rouzbahani
Mousa Kehtari
Mahdi Imani
Mehrdad Saviz
Iman Shabani
Farzad Moradikhah
Farhad Alizadeh
Mohammad-Hossein Karami
Ehsan Seyedjafari
Najmeh Jooyan
Zahra Mostajabi
Reza Faraji-Dana
author_sort Yashar Rouzbahani
collection DOAJ
description Weak microwave radiation (WMR) in our environment has raised health concerns in the public. Among those, communication frequencies are more than ever becoming widespread and their effects need thorough studies. A correct understanding of these effects in-vivo by in-vitro experiments shall preferentially use primary cells. In this study we compared non-modulated (CW) and modulated WMR exposure of biological cells in-vitro. Human ADMSC (Adipose-Derived Mesenchymal Stem Cells) were exposed to very weak non-thermal levels of microwave Electromagnetic fields at 1135 MHz ,SAR (Specific Absorption Rate) 0.002 W/kg (Watt per Kilogram) for 30 minutes daily for 4 days. A statistically significant decrease in proliferation rate of these stem cells was observed compared to the control group with no exposure. When amplitude-modulated exposure (15 Hz (Hertz) with a depth of 80%) was used with the same carrier frequency of 1135 MHz (Mega Hertz) and consistent average power, the cell numbers showed no statistically significant difference from the non-modulated exposure, but were nevertheless lower than the not-exposed control. The observed decrease in proliferation in response to -weak microwavefields supports the hypothesis that non-excitable cells, such as undifferentiated mesenchymal stem cells can interact with, and respond to weak electromagnetic radiation at communication frequencies. Possible mechanisms responsible for the observed results have been hypothesized and directions provided for future research.
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spelling doaj-art-67a17c0177d5419b8aa67526a65415f22025-08-20T03:31:52ZengAmirkabir University of TechnologyAUT Journal of Electrical Engineering2588-29102588-29292020-06-015213810.22060/eej.2020.16230.52833860Study of Ultra-weak CW and Amplitude-Modulated Microwaves effects on stem Cell Proliferation: an Experimental and Hypothetical ApproachYashar Rouzbahani0Mousa Kehtari1Mahdi Imani2Mehrdad Saviz3Iman Shabani4Farzad Moradikhah5Farhad Alizadeh6Mohammad-Hossein Karami7Ehsan Seyedjafari8Najmeh Jooyan9Zahra Mostajabi10Reza Faraji-Dana11Biomedical Engineering Department, Amirkabir University of Technology (Tehran Polytechnic), Tehran, IranDevelopmental Biology Laboratory, School of Biology, College of Science, University of Tehran, Tehran, IranBiomedical Engineering Department, Amirkabir University of Technology (Tehran Polytechnic), Tehran, IranBiomedical Engineering Department, Amirkabir University of Technology (Tehran Polytechnic)Biomedical Engineering Department, Amirkabir University of Technology (Tehran Polytechnic), Tehran, IranBiomedical Engineering Department, Amirkabir University of Technology (Tehran Polytechnic), Tehran, IranBiomedical Engineering Department, Amirkabir University of Technology (Tehran Polytechnic), Tehran, IranDevelopmental Biology Laboratory, School of Biology, College of Science, University of Tehran, Tehran, IranDepartment of Biotechnology, College of Science, University of Tehran, Tehran, IranBioelectromagnetics Laboratory, School of Electrical and Computer Engineering, University of TehranSchool of Electrical and Computer Engineering, University of Tehran, Tehran, IranINSF Chair of Computational Electromagnetics and Bio-electromagnetics, School of Electrical and Computer Engineering, University of Tehran, Tehran, Iran.Weak microwave radiation (WMR) in our environment has raised health concerns in the public. Among those, communication frequencies are more than ever becoming widespread and their effects need thorough studies. A correct understanding of these effects in-vivo by in-vitro experiments shall preferentially use primary cells. In this study we compared non-modulated (CW) and modulated WMR exposure of biological cells in-vitro. Human ADMSC (Adipose-Derived Mesenchymal Stem Cells) were exposed to very weak non-thermal levels of microwave Electromagnetic fields at 1135 MHz ,SAR (Specific Absorption Rate) 0.002 W/kg (Watt per Kilogram) for 30 minutes daily for 4 days. A statistically significant decrease in proliferation rate of these stem cells was observed compared to the control group with no exposure. When amplitude-modulated exposure (15 Hz (Hertz) with a depth of 80%) was used with the same carrier frequency of 1135 MHz (Mega Hertz) and consistent average power, the cell numbers showed no statistically significant difference from the non-modulated exposure, but were nevertheless lower than the not-exposed control. The observed decrease in proliferation in response to -weak microwavefields supports the hypothesis that non-excitable cells, such as undifferentiated mesenchymal stem cells can interact with, and respond to weak electromagnetic radiation at communication frequencies. Possible mechanisms responsible for the observed results have been hypothesized and directions provided for future research.https://eej.aut.ac.ir/article_3860_c17e42da17ed5b005b245ea8d617b512.pdfmicrowave em fieldsstem cellweak fieldnon-thermal effectsproliferation
spellingShingle Yashar Rouzbahani
Mousa Kehtari
Mahdi Imani
Mehrdad Saviz
Iman Shabani
Farzad Moradikhah
Farhad Alizadeh
Mohammad-Hossein Karami
Ehsan Seyedjafari
Najmeh Jooyan
Zahra Mostajabi
Reza Faraji-Dana
Study of Ultra-weak CW and Amplitude-Modulated Microwaves effects on stem Cell Proliferation: an Experimental and Hypothetical Approach
AUT Journal of Electrical Engineering
microwave em fields
stem cell
weak field
non-thermal effects
proliferation
title Study of Ultra-weak CW and Amplitude-Modulated Microwaves effects on stem Cell Proliferation: an Experimental and Hypothetical Approach
title_full Study of Ultra-weak CW and Amplitude-Modulated Microwaves effects on stem Cell Proliferation: an Experimental and Hypothetical Approach
title_fullStr Study of Ultra-weak CW and Amplitude-Modulated Microwaves effects on stem Cell Proliferation: an Experimental and Hypothetical Approach
title_full_unstemmed Study of Ultra-weak CW and Amplitude-Modulated Microwaves effects on stem Cell Proliferation: an Experimental and Hypothetical Approach
title_short Study of Ultra-weak CW and Amplitude-Modulated Microwaves effects on stem Cell Proliferation: an Experimental and Hypothetical Approach
title_sort study of ultra weak cw and amplitude modulated microwaves effects on stem cell proliferation an experimental and hypothetical approach
topic microwave em fields
stem cell
weak field
non-thermal effects
proliferation
url https://eej.aut.ac.ir/article_3860_c17e42da17ed5b005b245ea8d617b512.pdf
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