Engrafting gut bacteriophages have potential to modulate microbial metabolism in fecal microbiota transplantation
Abstract Background Fecal microbiota transplantation (FMT) is widely used to treat severe infections and investigated for the treatment of complex diseases. The therapeutic efficacy of FMT is related to the successful engraftment of bacteriophages from healthy donors to recipients. However, gut bact...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-06-01
|
| Series: | Microbiome |
| Online Access: | https://doi.org/10.1186/s40168-025-02046-5 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849329626484047872 |
|---|---|
| author | Shuai Ji Freed Ahmad Baizhao Peng Ying Yang Mengting Su Xiaoshan Zhao Tommi Vatanen |
| author_facet | Shuai Ji Freed Ahmad Baizhao Peng Ying Yang Mengting Su Xiaoshan Zhao Tommi Vatanen |
| author_sort | Shuai Ji |
| collection | DOAJ |
| description | Abstract Background Fecal microbiota transplantation (FMT) is widely used to treat severe infections and investigated for the treatment of complex diseases. The therapeutic efficacy of FMT is related to the successful engraftment of bacteriophages from healthy donors to recipients. However, gut bacteriophage contributions to FMT engraftment and treatment outcomes remain unclear. Methods The gut phageome from previously published metagenomes of donors and recipients across 23 FMT studies was assembled and functionally annotated for a meta-analysis. Results Gut phageome profiles of FMT recipients, especially those with recurrent Clostridioides difficile infection (rCDI), shifted toward donor phageomes, accompanied by increased phageome alpha diversity. Engraftment of donor phages varied between recipient conditions with the highest engraftment rate, overrepresented by putative temperate phage, in patients with rCDI. Consistently, a higher proportion of auxiliary metabolic genes (AMGs), with the potential to support and modulate bacterial metabolism, were annotated on putative temperate phages. Conclusions FMT leads to significant taxonomic, functional, and lifestyle shifts in recipient phageome composition. Future FMT studies should include gut phageome characterization and consider it as a potential factor in microbial community shifts and treatment outcomes. Video Abstract |
| format | Article |
| id | doaj-art-679eb7a8bfd64159bee2b7efd03568a8 |
| institution | Kabale University |
| issn | 2049-2618 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbiome |
| spelling | doaj-art-679eb7a8bfd64159bee2b7efd03568a82025-08-20T03:47:13ZengBMCMicrobiome2049-26182025-06-0113111610.1186/s40168-025-02046-5Engrafting gut bacteriophages have potential to modulate microbial metabolism in fecal microbiota transplantationShuai Ji0Freed Ahmad1Baizhao Peng2Ying Yang3Mengting Su4Xiaoshan Zhao5Tommi Vatanen6Nanfang Hospital, Southern Medical UniversityInstitute of Biotechnology, Helsinki Institute of Life Science, University of HelsinkiNanfang Hospital, Southern Medical UniversityNanfang Hospital, Southern Medical UniversityCellular & Molecular Diagnostics Center, Sun Yat-Sen Memorial Hospital of Sun Yat-Sen UniversityNanfang Hospital, Southern Medical UniversityInstitute of Biotechnology, Helsinki Institute of Life Science, University of HelsinkiAbstract Background Fecal microbiota transplantation (FMT) is widely used to treat severe infections and investigated for the treatment of complex diseases. The therapeutic efficacy of FMT is related to the successful engraftment of bacteriophages from healthy donors to recipients. However, gut bacteriophage contributions to FMT engraftment and treatment outcomes remain unclear. Methods The gut phageome from previously published metagenomes of donors and recipients across 23 FMT studies was assembled and functionally annotated for a meta-analysis. Results Gut phageome profiles of FMT recipients, especially those with recurrent Clostridioides difficile infection (rCDI), shifted toward donor phageomes, accompanied by increased phageome alpha diversity. Engraftment of donor phages varied between recipient conditions with the highest engraftment rate, overrepresented by putative temperate phage, in patients with rCDI. Consistently, a higher proportion of auxiliary metabolic genes (AMGs), with the potential to support and modulate bacterial metabolism, were annotated on putative temperate phages. Conclusions FMT leads to significant taxonomic, functional, and lifestyle shifts in recipient phageome composition. Future FMT studies should include gut phageome characterization and consider it as a potential factor in microbial community shifts and treatment outcomes. Video Abstracthttps://doi.org/10.1186/s40168-025-02046-5 |
| spellingShingle | Shuai Ji Freed Ahmad Baizhao Peng Ying Yang Mengting Su Xiaoshan Zhao Tommi Vatanen Engrafting gut bacteriophages have potential to modulate microbial metabolism in fecal microbiota transplantation Microbiome |
| title | Engrafting gut bacteriophages have potential to modulate microbial metabolism in fecal microbiota transplantation |
| title_full | Engrafting gut bacteriophages have potential to modulate microbial metabolism in fecal microbiota transplantation |
| title_fullStr | Engrafting gut bacteriophages have potential to modulate microbial metabolism in fecal microbiota transplantation |
| title_full_unstemmed | Engrafting gut bacteriophages have potential to modulate microbial metabolism in fecal microbiota transplantation |
| title_short | Engrafting gut bacteriophages have potential to modulate microbial metabolism in fecal microbiota transplantation |
| title_sort | engrafting gut bacteriophages have potential to modulate microbial metabolism in fecal microbiota transplantation |
| url | https://doi.org/10.1186/s40168-025-02046-5 |
| work_keys_str_mv | AT shuaiji engraftinggutbacteriophageshavepotentialtomodulatemicrobialmetabolisminfecalmicrobiotatransplantation AT freedahmad engraftinggutbacteriophageshavepotentialtomodulatemicrobialmetabolisminfecalmicrobiotatransplantation AT baizhaopeng engraftinggutbacteriophageshavepotentialtomodulatemicrobialmetabolisminfecalmicrobiotatransplantation AT yingyang engraftinggutbacteriophageshavepotentialtomodulatemicrobialmetabolisminfecalmicrobiotatransplantation AT mengtingsu engraftinggutbacteriophageshavepotentialtomodulatemicrobialmetabolisminfecalmicrobiotatransplantation AT xiaoshanzhao engraftinggutbacteriophageshavepotentialtomodulatemicrobialmetabolisminfecalmicrobiotatransplantation AT tommivatanen engraftinggutbacteriophageshavepotentialtomodulatemicrobialmetabolisminfecalmicrobiotatransplantation |