Hard tissue formation after direct pulp capping with osteostatin and MTA in vivo
ObjectivesIn recent in vitro study, it was reported that osteostatin (OST) has an odontogenic effect and synergistic effect with mineral trioxide aggregate (MTA) in human dental pulp cells. Therefore, the aim of this study was to evaluate whether OST has a synergistic effect with MTA on hard tissue...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Korean Academy of Conservative Dentistry
2021-05-01
|
| Series: | Restorative Dentistry & Endodontics |
| Subjects: | |
| Online Access: | http://rde.ac/upload/pdf/rde-46-e17.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849428103696220160 |
|---|---|
| author | Ji-Hye Yoon Sung-Hyeon Choi Jeong-Tae Koh Bin-Na Lee Hoon-Sang Chang In-Nam Hwang Won-Mann Oh Yun-Chan Hwang |
| author_facet | Ji-Hye Yoon Sung-Hyeon Choi Jeong-Tae Koh Bin-Na Lee Hoon-Sang Chang In-Nam Hwang Won-Mann Oh Yun-Chan Hwang |
| author_sort | Ji-Hye Yoon |
| collection | DOAJ |
| description | ObjectivesIn recent in vitro study, it was reported that osteostatin (OST) has an odontogenic effect and synergistic effect with mineral trioxide aggregate (MTA) in human dental pulp cells. Therefore, the aim of this study was to evaluate whether OST has a synergistic effect with MTA on hard tissue formation in vivo.Materials and MethodsThirty-two maxillary molars of Spraque-Dawley rats were used in this study. An occlusal cavity was prepared and the exposed pulps were randomly divided into 3 groups: group 1 (control; ProRoot MTA), group 2 (OST 100 μM + ProRoot MTA), group 3 (OST 10 mM + ProRoot MTA). Exposed pulps were capped with each material and cavities were restored with resin modified glass ionomer. The animals were sacrificed after 4 weeks. All harvested teeth were scanned with micro-computed tomography (CT). The samples were prepared and hard tissue formation was evaluated histologically. For immunohistochemical analysis, the specimens were sectioned and incubated with primary antibodies against dentin sialoprotein (DSP).ResultsIn the micro-CT analysis, it is revealed that OST with ProRoot MTA groups showed more mineralized bridge than the control (p < 0.05). In the H&E staining, it is showed that more quantity of the mineralized dentin bridge was formed in the OST with ProRoot MTA group compared to the control (p < 0.05). In all groups, DSP was expressed in newly formed reparative dentin area.ConclusionsOST can be a supplementary pulp capping material when used with MTA to make synergistic effect in hard tissue formation. |
| format | Article |
| id | doaj-art-6788667e044d4b89b822e73a8f70967c |
| institution | Kabale University |
| issn | 2234-7658 2234-7666 |
| language | English |
| publishDate | 2021-05-01 |
| publisher | Korean Academy of Conservative Dentistry |
| record_format | Article |
| series | Restorative Dentistry & Endodontics |
| spelling | doaj-art-6788667e044d4b89b822e73a8f70967c2025-08-20T03:28:48ZengKorean Academy of Conservative DentistryRestorative Dentistry & Endodontics2234-76582234-76662021-05-01462e1710.5395/rde.2021.46.e17955Hard tissue formation after direct pulp capping with osteostatin and MTA in vivoJi-Hye Yoon0Sung-Hyeon Choi1Jeong-Tae Koh2Bin-Na Lee3Hoon-Sang Chang4In-Nam Hwang5Won-Mann Oh6Yun-Chan Hwang7Department of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, KoreaDepartment of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, KoreaDepartment of Pharmacology and Dental Therapeutics, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, KoreaDepartment of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, KoreaDepartment of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, KoreaDepartment of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, KoreaDepartment of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, KoreaDepartment of Conservative Dentistry, School of Dentistry, Dental Science Research Institute, Chonnam National University, Gwangju, KoreaObjectivesIn recent in vitro study, it was reported that osteostatin (OST) has an odontogenic effect and synergistic effect with mineral trioxide aggregate (MTA) in human dental pulp cells. Therefore, the aim of this study was to evaluate whether OST has a synergistic effect with MTA on hard tissue formation in vivo.Materials and MethodsThirty-two maxillary molars of Spraque-Dawley rats were used in this study. An occlusal cavity was prepared and the exposed pulps were randomly divided into 3 groups: group 1 (control; ProRoot MTA), group 2 (OST 100 μM + ProRoot MTA), group 3 (OST 10 mM + ProRoot MTA). Exposed pulps were capped with each material and cavities were restored with resin modified glass ionomer. The animals were sacrificed after 4 weeks. All harvested teeth were scanned with micro-computed tomography (CT). The samples were prepared and hard tissue formation was evaluated histologically. For immunohistochemical analysis, the specimens were sectioned and incubated with primary antibodies against dentin sialoprotein (DSP).ResultsIn the micro-CT analysis, it is revealed that OST with ProRoot MTA groups showed more mineralized bridge than the control (p < 0.05). In the H&E staining, it is showed that more quantity of the mineralized dentin bridge was formed in the OST with ProRoot MTA group compared to the control (p < 0.05). In all groups, DSP was expressed in newly formed reparative dentin area.ConclusionsOST can be a supplementary pulp capping material when used with MTA to make synergistic effect in hard tissue formation.http://rde.ac/upload/pdf/rde-46-e17.pdfdirect pulp cappingmineralization, mtaosteostatin |
| spellingShingle | Ji-Hye Yoon Sung-Hyeon Choi Jeong-Tae Koh Bin-Na Lee Hoon-Sang Chang In-Nam Hwang Won-Mann Oh Yun-Chan Hwang Hard tissue formation after direct pulp capping with osteostatin and MTA in vivo Restorative Dentistry & Endodontics direct pulp capping mineralization, mta osteostatin |
| title | Hard tissue formation after direct pulp capping with osteostatin and MTA in vivo |
| title_full | Hard tissue formation after direct pulp capping with osteostatin and MTA in vivo |
| title_fullStr | Hard tissue formation after direct pulp capping with osteostatin and MTA in vivo |
| title_full_unstemmed | Hard tissue formation after direct pulp capping with osteostatin and MTA in vivo |
| title_short | Hard tissue formation after direct pulp capping with osteostatin and MTA in vivo |
| title_sort | hard tissue formation after direct pulp capping with osteostatin and mta in vivo |
| topic | direct pulp capping mineralization, mta osteostatin |
| url | http://rde.ac/upload/pdf/rde-46-e17.pdf |
| work_keys_str_mv | AT jihyeyoon hardtissueformationafterdirectpulpcappingwithosteostatinandmtainvivo AT sunghyeonchoi hardtissueformationafterdirectpulpcappingwithosteostatinandmtainvivo AT jeongtaekoh hardtissueformationafterdirectpulpcappingwithosteostatinandmtainvivo AT binnalee hardtissueformationafterdirectpulpcappingwithosteostatinandmtainvivo AT hoonsangchang hardtissueformationafterdirectpulpcappingwithosteostatinandmtainvivo AT innamhwang hardtissueformationafterdirectpulpcappingwithosteostatinandmtainvivo AT wonmannoh hardtissueformationafterdirectpulpcappingwithosteostatinandmtainvivo AT yunchanhwang hardtissueformationafterdirectpulpcappingwithosteostatinandmtainvivo |