Preparation and quality detection on gene chip of head cDNA from Apis cerana cerana
Through sequencing of the head cDNA library of Chinese honeybee (Apis cerana cerana) and sequence analysis, 8569 high quality EST sequences were obtained. Based on the function annotation, 1025 candidate ESTs used in gene chip were selected to set up database. With the plasmids extracted from the cl...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2009-01-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2009.01.004 |
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| Summary: | Through sequencing of the head cDNA library of Chinese honeybee (Apis cerana cerana) and sequence analysis, 8569 high quality EST sequences were obtained. Based on the function annotation, 1025 candidate ESTs used in gene chip were selected to set up database. With the plasmids extracted from the clones of the cDNA library which were selected according to candidate EST code as templates, 717 valid EST probers were amplified with PCR reaction and detected with gel electrophoresis. These purified ESTs were printed into cDNA chip, which was hybridized with the fluorescent labeling worker head cDNA samples of A. mellifera and A. cerana cerana. The image-scanned and scatter plot analyzed results showed that hybridization signals were normal. The research set up a solid base for studying and digging up honeybee functional genes further. |
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| ISSN: | 1008-9209 2097-5155 |