Comparative Study on the Effects of Four Plant Protein Sources on the Liver and Intestinal Health of Largemouth Bass, Micropterus salmoides
The effects of plant protein sources (PPSs) on the health of the liver and intestine of the largemouth bass, Micropterus salmoides, were compared to verify the potential damaging effects of dietary fiber (DF). A diet containing 55% fish meal (FM) was used as the control. The test diets contained 25%...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2024-01-01
|
| Series: | Aquaculture Nutrition |
| Online Access: | http://dx.doi.org/10.1155/2024/6337005 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832558594499805184 |
|---|---|
| author | Shibin Yao Wenjian Li Chunfang Cai Chengrui Wang Jia Kang Honglin Hu Ping Wu Xiamin Cao Yuantu Ye |
| author_facet | Shibin Yao Wenjian Li Chunfang Cai Chengrui Wang Jia Kang Honglin Hu Ping Wu Xiamin Cao Yuantu Ye |
| author_sort | Shibin Yao |
| collection | DOAJ |
| description | The effects of plant protein sources (PPSs) on the health of the liver and intestine of the largemouth bass, Micropterus salmoides, were compared to verify the potential damaging effects of dietary fiber (DF). A diet containing 55% fish meal (FM) was used as the control. The test diets contained 25% soybean meal (SBM), rapeseed meal (RSM), cottonseed meal, or peanut meal, and the FM content was decreased to 30%. The protein and lipid contents of these five diets were balanced by casein and oil. Fish were raised for 8 weeks. The fish fed the diet containing PPS showed a trend of decreasing growth and apparent digestibility coefficients. The contents of total bile acid, lipid, and collagen in the liver were increased, and the mRNA expression levels of genes encoding inflammatory factors and enzymes involved in de novo fatty acid synthesis and bile acid synthesis were upregulated. Both the lipid and collagen contents in the liver were positively correlated with the DF content in the diet significantly. Morphology and histology showed reduced liver size, hepatic steatosis, and fibrosis in fish fed diets containing PPS. The lowest hepatosomatic index was observed in fish fed the SBM diet, and the most severe damage was observed in fish fed the RSM diet. No obvious histological abnormalities were observed in the hindgut. The bile acid profile in the liver could be used to distinguish the types of PPS very well by Fisher discriminant analysis. These results indicated that 25% of each of the four PPSs in the diet exceeded the tolerance range of largemouth bass and caused liver damage, which might be mediated by bile acid. DF in PPS might be an important agent contributing to liver damage. |
| format | Article |
| id | doaj-art-678569c468454e4eac59f7fa84265763 |
| institution | Kabale University |
| issn | 1365-2095 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Aquaculture Nutrition |
| spelling | doaj-art-678569c468454e4eac59f7fa842657632025-02-03T01:31:58ZengWileyAquaculture Nutrition1365-20952024-01-01202410.1155/2024/6337005Comparative Study on the Effects of Four Plant Protein Sources on the Liver and Intestinal Health of Largemouth Bass, Micropterus salmoidesShibin Yao0Wenjian Li1Chunfang Cai2Chengrui Wang3Jia Kang4Honglin Hu5Ping Wu6Xiamin Cao7Yuantu Ye8Key Laboratory of Aquatic Animal Nutrition of JiangsuKey Laboratory of Aquatic Animal Nutrition of JiangsuKey Laboratory of Aquatic Animal Nutrition of JiangsuKey Laboratory of Aquatic Animal Nutrition of JiangsuKey Laboratory of Aquatic Animal Nutrition of JiangsuKey Laboratory of Aquatic Animal Nutrition of JiangsuKey Laboratory of Aquatic Animal Nutrition of JiangsuKey Laboratory of Aquatic Animal Nutrition of JiangsuKey Laboratory of Aquatic Animal Nutrition of JiangsuThe effects of plant protein sources (PPSs) on the health of the liver and intestine of the largemouth bass, Micropterus salmoides, were compared to verify the potential damaging effects of dietary fiber (DF). A diet containing 55% fish meal (FM) was used as the control. The test diets contained 25% soybean meal (SBM), rapeseed meal (RSM), cottonseed meal, or peanut meal, and the FM content was decreased to 30%. The protein and lipid contents of these five diets were balanced by casein and oil. Fish were raised for 8 weeks. The fish fed the diet containing PPS showed a trend of decreasing growth and apparent digestibility coefficients. The contents of total bile acid, lipid, and collagen in the liver were increased, and the mRNA expression levels of genes encoding inflammatory factors and enzymes involved in de novo fatty acid synthesis and bile acid synthesis were upregulated. Both the lipid and collagen contents in the liver were positively correlated with the DF content in the diet significantly. Morphology and histology showed reduced liver size, hepatic steatosis, and fibrosis in fish fed diets containing PPS. The lowest hepatosomatic index was observed in fish fed the SBM diet, and the most severe damage was observed in fish fed the RSM diet. No obvious histological abnormalities were observed in the hindgut. The bile acid profile in the liver could be used to distinguish the types of PPS very well by Fisher discriminant analysis. These results indicated that 25% of each of the four PPSs in the diet exceeded the tolerance range of largemouth bass and caused liver damage, which might be mediated by bile acid. DF in PPS might be an important agent contributing to liver damage.http://dx.doi.org/10.1155/2024/6337005 |
| spellingShingle | Shibin Yao Wenjian Li Chunfang Cai Chengrui Wang Jia Kang Honglin Hu Ping Wu Xiamin Cao Yuantu Ye Comparative Study on the Effects of Four Plant Protein Sources on the Liver and Intestinal Health of Largemouth Bass, Micropterus salmoides Aquaculture Nutrition |
| title | Comparative Study on the Effects of Four Plant Protein Sources on the Liver and Intestinal Health of Largemouth Bass, Micropterus salmoides |
| title_full | Comparative Study on the Effects of Four Plant Protein Sources on the Liver and Intestinal Health of Largemouth Bass, Micropterus salmoides |
| title_fullStr | Comparative Study on the Effects of Four Plant Protein Sources on the Liver and Intestinal Health of Largemouth Bass, Micropterus salmoides |
| title_full_unstemmed | Comparative Study on the Effects of Four Plant Protein Sources on the Liver and Intestinal Health of Largemouth Bass, Micropterus salmoides |
| title_short | Comparative Study on the Effects of Four Plant Protein Sources on the Liver and Intestinal Health of Largemouth Bass, Micropterus salmoides |
| title_sort | comparative study on the effects of four plant protein sources on the liver and intestinal health of largemouth bass micropterus salmoides |
| url | http://dx.doi.org/10.1155/2024/6337005 |
| work_keys_str_mv | AT shibinyao comparativestudyontheeffectsoffourplantproteinsourcesontheliverandintestinalhealthoflargemouthbassmicropterussalmoides AT wenjianli comparativestudyontheeffectsoffourplantproteinsourcesontheliverandintestinalhealthoflargemouthbassmicropterussalmoides AT chunfangcai comparativestudyontheeffectsoffourplantproteinsourcesontheliverandintestinalhealthoflargemouthbassmicropterussalmoides AT chengruiwang comparativestudyontheeffectsoffourplantproteinsourcesontheliverandintestinalhealthoflargemouthbassmicropterussalmoides AT jiakang comparativestudyontheeffectsoffourplantproteinsourcesontheliverandintestinalhealthoflargemouthbassmicropterussalmoides AT honglinhu comparativestudyontheeffectsoffourplantproteinsourcesontheliverandintestinalhealthoflargemouthbassmicropterussalmoides AT pingwu comparativestudyontheeffectsoffourplantproteinsourcesontheliverandintestinalhealthoflargemouthbassmicropterussalmoides AT xiamincao comparativestudyontheeffectsoffourplantproteinsourcesontheliverandintestinalhealthoflargemouthbassmicropterussalmoides AT yuantuye comparativestudyontheeffectsoffourplantproteinsourcesontheliverandintestinalhealthoflargemouthbassmicropterussalmoides |