Enzyme Inhibitory Properties, Antioxidant Activities, and Phytochemical Profile of Three Medicinal Plants from Turkey
We aimed to investigate the inhibitory potential of three medicinal plants (Hedysarum varium, Onobrychis hypargyrea, and Vicia truncatula) from Turkey against key enzymes involved in human pathologies, namely, diabetes (α-amylase and α-glucosidase), neurodegenerative disorders (tyrosinase, acetylcho...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Advances in Pharmacological Sciences |
| Online Access: | http://dx.doi.org/10.1155/2015/410675 |
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| author | Gokhan Zengin Gokalp Ozmen Guler Abdurrahman Aktumsek Ramazan Ceylan Carene Marie Nancy Picot M. Fawzi Mahomoodally |
| author_facet | Gokhan Zengin Gokalp Ozmen Guler Abdurrahman Aktumsek Ramazan Ceylan Carene Marie Nancy Picot M. Fawzi Mahomoodally |
| author_sort | Gokhan Zengin |
| collection | DOAJ |
| description | We aimed to investigate the inhibitory potential of three medicinal plants (Hedysarum varium, Onobrychis hypargyrea, and Vicia truncatula) from Turkey against key enzymes involved in human pathologies, namely, diabetes (α-amylase and α-glucosidase), neurodegenerative disorders (tyrosinase, acetylcholinesterase, and butyrylcholinesterase), and hyperpigmentation (tyrosinase). The antioxidant potential, phenolic and flavonoid content of ethyl acetate, and methanolic and aqueous extracts were investigated using in vitro assays. The total antioxidant capacity (TAC), β-carotene/linoleic acid bleaching activity, 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH•), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+), cupric ion reducing antioxidant capacity (CUPRAC), ferric reducing antioxidant power (FRAP), and metal chelating activity on ferrous ions were used to evaluate the antioxidant capabilities of the extracts. The half-maximal inhibitory concentrations (IC50) of the extracts on cholinesterase, tyrosinase, and α-amylase were significantly higher than the references, galantamine, kojic acid, and acarbose, respectively. The half-maximal effective concentrations (EC50) of the extracts on TAC, CUPRAC, and FRAP were significantly higher than trolox. The phenol and flavonoid contents of the plant extracts were in the range 20.90±0.190–83.25±0.914 mg gallic acid equivalent/g extract and 1.45±0.200–39.71±0.092 mg rutin equivalent/g extract, respectively. The plants were found to possess moderate antioxidant capacities and interesting inhibitory action against key enzymes. |
| format | Article |
| id | doaj-art-67808739a8db4a4faaaf94b8a31fcaf8 |
| institution | OA Journals |
| issn | 1687-6334 1687-6342 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Pharmacological Sciences |
| spelling | doaj-art-67808739a8db4a4faaaf94b8a31fcaf82025-08-20T02:23:49ZengWileyAdvances in Pharmacological Sciences1687-63341687-63422015-01-01201510.1155/2015/410675410675Enzyme Inhibitory Properties, Antioxidant Activities, and Phytochemical Profile of Three Medicinal Plants from TurkeyGokhan Zengin0Gokalp Ozmen Guler1Abdurrahman Aktumsek2Ramazan Ceylan3Carene Marie Nancy Picot4M. Fawzi Mahomoodally5Department of Biology, Science Faculty, Selçuk University Campus, 42250 Konya, TurkeyDepartment of Biological Education, Ahmet Keleşoğlu Education Faculty, Necmettin Erbakan University, 42090 Konya, TurkeyDepartment of Biology, Science Faculty, Selçuk University Campus, 42250 Konya, TurkeyDepartment of Biology, Science Faculty, Selçuk University Campus, 42250 Konya, TurkeyDepartment of Health Sciences, Faculty of Science, University of Mauritius, 230 Réduit, MauritiusDepartment of Health Sciences, Faculty of Science, University of Mauritius, 230 Réduit, MauritiusWe aimed to investigate the inhibitory potential of three medicinal plants (Hedysarum varium, Onobrychis hypargyrea, and Vicia truncatula) from Turkey against key enzymes involved in human pathologies, namely, diabetes (α-amylase and α-glucosidase), neurodegenerative disorders (tyrosinase, acetylcholinesterase, and butyrylcholinesterase), and hyperpigmentation (tyrosinase). The antioxidant potential, phenolic and flavonoid content of ethyl acetate, and methanolic and aqueous extracts were investigated using in vitro assays. The total antioxidant capacity (TAC), β-carotene/linoleic acid bleaching activity, 1,1-diphenyl-2-picrylhydrazyl free radical (DPPH•), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+), cupric ion reducing antioxidant capacity (CUPRAC), ferric reducing antioxidant power (FRAP), and metal chelating activity on ferrous ions were used to evaluate the antioxidant capabilities of the extracts. The half-maximal inhibitory concentrations (IC50) of the extracts on cholinesterase, tyrosinase, and α-amylase were significantly higher than the references, galantamine, kojic acid, and acarbose, respectively. The half-maximal effective concentrations (EC50) of the extracts on TAC, CUPRAC, and FRAP were significantly higher than trolox. The phenol and flavonoid contents of the plant extracts were in the range 20.90±0.190–83.25±0.914 mg gallic acid equivalent/g extract and 1.45±0.200–39.71±0.092 mg rutin equivalent/g extract, respectively. The plants were found to possess moderate antioxidant capacities and interesting inhibitory action against key enzymes.http://dx.doi.org/10.1155/2015/410675 |
| spellingShingle | Gokhan Zengin Gokalp Ozmen Guler Abdurrahman Aktumsek Ramazan Ceylan Carene Marie Nancy Picot M. Fawzi Mahomoodally Enzyme Inhibitory Properties, Antioxidant Activities, and Phytochemical Profile of Three Medicinal Plants from Turkey Advances in Pharmacological Sciences |
| title | Enzyme Inhibitory Properties, Antioxidant Activities, and Phytochemical Profile of Three Medicinal Plants from Turkey |
| title_full | Enzyme Inhibitory Properties, Antioxidant Activities, and Phytochemical Profile of Three Medicinal Plants from Turkey |
| title_fullStr | Enzyme Inhibitory Properties, Antioxidant Activities, and Phytochemical Profile of Three Medicinal Plants from Turkey |
| title_full_unstemmed | Enzyme Inhibitory Properties, Antioxidant Activities, and Phytochemical Profile of Three Medicinal Plants from Turkey |
| title_short | Enzyme Inhibitory Properties, Antioxidant Activities, and Phytochemical Profile of Three Medicinal Plants from Turkey |
| title_sort | enzyme inhibitory properties antioxidant activities and phytochemical profile of three medicinal plants from turkey |
| url | http://dx.doi.org/10.1155/2015/410675 |
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