CRM197-conjugated peptides vaccine of HCMV pp65 and gH induce maturation of DC and effective viral-specific T cell responses
Human cytomegalovirus (HCMV) infection is prevalent worldwide, and there is currently no licenced HCMV vaccine to control it. Therefore, developing an effective HCMV vaccine is a significant priority. Because of their excellent immunogenicity, the crucial components of HCMV, phosphoprotein 65 (pp65)...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2023-12-01
|
| Series: | Virulence |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21505594.2023.2169488 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849418909902438400 |
|---|---|
| author | Shuyun Zhang Fulong Nan Shasha Jiang Xiaoqiong Zhou Delei Niu Jun Li Hui Wang Xueming Zhang Xianjuan Zhang Bin Wang |
| author_facet | Shuyun Zhang Fulong Nan Shasha Jiang Xiaoqiong Zhou Delei Niu Jun Li Hui Wang Xueming Zhang Xianjuan Zhang Bin Wang |
| author_sort | Shuyun Zhang |
| collection | DOAJ |
| description | Human cytomegalovirus (HCMV) infection is prevalent worldwide, and there is currently no licenced HCMV vaccine to control it. Therefore, developing an effective HCMV vaccine is a significant priority. Because of their excellent immunogenicity, the crucial components of HCMV, phosphoprotein 65 (pp65) and glycoproteins H (gH) are potential target proteins for HCMV vaccine design. In this study, we predicted and screened the dominant antigenic epitopes of B and T cells from pp65 and gH conjugated with the carrier protein cross-reacting material 197 (CRM197) to form three peptide-CRM197 vaccines (pp65-CRM197, gH-CRM197, and pp65-CRM197+gH-CRM197). Furthermore, the immunogenicity of the peptide-CRM197 vaccines and their effects on dendritic cells (DCs) were explored. The results showed that three peptide-CRM197 vaccines could induce maturation of DCs through the p38 MAPK signalling pathway and promote the release of proinflammatory factors, such as TNF-α and interleukin (IL) −6. Meanwhile, the peptide-CRM197 vaccines could effectively activate T cell and humoral immunity, which were far better than the inactivated HCMV vaccine. In conclusion, we constructed three peptide-CRM197 vaccines, which could induce multiple immune effects, providing a novel approach for HCMV vaccine design. |
| format | Article |
| id | doaj-art-6777caa65d0f4bf88023cebed8bf6b34 |
| institution | Kabale University |
| issn | 2150-5594 2150-5608 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj-art-6777caa65d0f4bf88023cebed8bf6b342025-08-20T03:32:18ZengTaylor & Francis GroupVirulence2150-55942150-56082023-12-0114110.1080/21505594.2023.2169488CRM197-conjugated peptides vaccine of HCMV pp65 and gH induce maturation of DC and effective viral-specific T cell responsesShuyun Zhang0Fulong Nan1Shasha Jiang2Xiaoqiong Zhou3Delei Niu4Jun Li5Hui Wang6Xueming Zhang7Xianjuan Zhang8Bin Wang9Department of Pathogenic Biology, Department of Special Medicine, School of Basic Medicine, Qingdao University, Qingdao, ChinaDepartment of Pathogenic Biology, Department of Special Medicine, School of Basic Medicine, Qingdao University, Qingdao, ChinaDepartment of Pathogenic Biology, Department of Special Medicine, School of Basic Medicine, Qingdao University, Qingdao, ChinaDepartment of Pathogenic Biology, Department of Special Medicine, School of Basic Medicine, Qingdao University, Qingdao, ChinaDepartment of Pathogenic Biology, Department of Special Medicine, School of Basic Medicine, Qingdao University, Qingdao, ChinaDepartment of Pathogenic Biology, Department of Special Medicine, School of Basic Medicine, Qingdao University, Qingdao, ChinaDepartment of Pathogenic Biology, Department of Special Medicine, School of Basic Medicine, Qingdao University, Qingdao, ChinaDepartment of Pathogenic Biology, Department of Special Medicine, School of Basic Medicine, Qingdao University, Qingdao, ChinaDepartment of Pathogenic Biology, Department of Special Medicine, School of Basic Medicine, Qingdao University, Qingdao, ChinaDepartment of Pathogenic Biology, Department of Special Medicine, School of Basic Medicine, Qingdao University, Qingdao, ChinaHuman cytomegalovirus (HCMV) infection is prevalent worldwide, and there is currently no licenced HCMV vaccine to control it. Therefore, developing an effective HCMV vaccine is a significant priority. Because of their excellent immunogenicity, the crucial components of HCMV, phosphoprotein 65 (pp65) and glycoproteins H (gH) are potential target proteins for HCMV vaccine design. In this study, we predicted and screened the dominant antigenic epitopes of B and T cells from pp65 and gH conjugated with the carrier protein cross-reacting material 197 (CRM197) to form three peptide-CRM197 vaccines (pp65-CRM197, gH-CRM197, and pp65-CRM197+gH-CRM197). Furthermore, the immunogenicity of the peptide-CRM197 vaccines and their effects on dendritic cells (DCs) were explored. The results showed that three peptide-CRM197 vaccines could induce maturation of DCs through the p38 MAPK signalling pathway and promote the release of proinflammatory factors, such as TNF-α and interleukin (IL) −6. Meanwhile, the peptide-CRM197 vaccines could effectively activate T cell and humoral immunity, which were far better than the inactivated HCMV vaccine. In conclusion, we constructed three peptide-CRM197 vaccines, which could induce multiple immune effects, providing a novel approach for HCMV vaccine design.https://www.tandfonline.com/doi/10.1080/21505594.2023.2169488Human cytomegaloviruspeptide-CRM197 vaccineantigen presentationmaturation of DCsT cell immunity |
| spellingShingle | Shuyun Zhang Fulong Nan Shasha Jiang Xiaoqiong Zhou Delei Niu Jun Li Hui Wang Xueming Zhang Xianjuan Zhang Bin Wang CRM197-conjugated peptides vaccine of HCMV pp65 and gH induce maturation of DC and effective viral-specific T cell responses Virulence Human cytomegalovirus peptide-CRM197 vaccine antigen presentation maturation of DCs T cell immunity |
| title | CRM197-conjugated peptides vaccine of HCMV pp65 and gH induce maturation of DC and effective viral-specific T cell responses |
| title_full | CRM197-conjugated peptides vaccine of HCMV pp65 and gH induce maturation of DC and effective viral-specific T cell responses |
| title_fullStr | CRM197-conjugated peptides vaccine of HCMV pp65 and gH induce maturation of DC and effective viral-specific T cell responses |
| title_full_unstemmed | CRM197-conjugated peptides vaccine of HCMV pp65 and gH induce maturation of DC and effective viral-specific T cell responses |
| title_short | CRM197-conjugated peptides vaccine of HCMV pp65 and gH induce maturation of DC and effective viral-specific T cell responses |
| title_sort | crm197 conjugated peptides vaccine of hcmv pp65 and gh induce maturation of dc and effective viral specific t cell responses |
| topic | Human cytomegalovirus peptide-CRM197 vaccine antigen presentation maturation of DCs T cell immunity |
| url | https://www.tandfonline.com/doi/10.1080/21505594.2023.2169488 |
| work_keys_str_mv | AT shuyunzhang crm197conjugatedpeptidesvaccineofhcmvpp65andghinducematurationofdcandeffectiveviralspecifictcellresponses AT fulongnan crm197conjugatedpeptidesvaccineofhcmvpp65andghinducematurationofdcandeffectiveviralspecifictcellresponses AT shashajiang crm197conjugatedpeptidesvaccineofhcmvpp65andghinducematurationofdcandeffectiveviralspecifictcellresponses AT xiaoqiongzhou crm197conjugatedpeptidesvaccineofhcmvpp65andghinducematurationofdcandeffectiveviralspecifictcellresponses AT deleiniu crm197conjugatedpeptidesvaccineofhcmvpp65andghinducematurationofdcandeffectiveviralspecifictcellresponses AT junli crm197conjugatedpeptidesvaccineofhcmvpp65andghinducematurationofdcandeffectiveviralspecifictcellresponses AT huiwang crm197conjugatedpeptidesvaccineofhcmvpp65andghinducematurationofdcandeffectiveviralspecifictcellresponses AT xuemingzhang crm197conjugatedpeptidesvaccineofhcmvpp65andghinducematurationofdcandeffectiveviralspecifictcellresponses AT xianjuanzhang crm197conjugatedpeptidesvaccineofhcmvpp65andghinducematurationofdcandeffectiveviralspecifictcellresponses AT binwang crm197conjugatedpeptidesvaccineofhcmvpp65andghinducematurationofdcandeffectiveviralspecifictcellresponses |