Antimicrobial potential and mechanism of infant feces-derived Enterococcus faecalis in inhibiting Enterotoxigenic Escherichia coli
Enterotoxigenic Escherichia coli (ETEC) is a leading cause of diarrhea in infants and travelers. However, antibiotic treatment is challenged by growing resistance. This study investigated the potential of Enterococcus faecalis, an early colonizer of the infant gut, as a probiotic to combat ETEC. Fro...
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| Language: | English |
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Elsevier
2025-01-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464625000015 |
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| author | Xi Lu Wei Sang Lei Pan |
| author_facet | Xi Lu Wei Sang Lei Pan |
| author_sort | Xi Lu |
| collection | DOAJ |
| description | Enterotoxigenic Escherichia coli (ETEC) is a leading cause of diarrhea in infants and travelers. However, antibiotic treatment is challenged by growing resistance. This study investigated the potential of Enterococcus faecalis, an early colonizer of the infant gut, as a probiotic to combat ETEC. From 125 healthy infant feces, 54 E. faecalis strains were isolated and evaluated for safety and function. E. faecalis LX25 and LX39 showed significant inhibition of ETEC growth, virulence gene (eltA and stA) expression (P < 0.05) and disrupted biofilm formation. Notably, LX39's cell-free supernatant (CFS) increased reactive oxygen species in ETEC, leading to cellular damage. HPLC revealed the three most concentrated organic acids, lactic (10.31 mg/mL), acetic (1.72 mg/mL) and citric acids (1.58 mg/mL) in LX39's CFS, which exceeding or approaching the MIC for ETEC. These findings indicate that E. faecalis LX39 could serve as effective probiotics candidates to inhibit ETEC, highlighting their potential in managing intestinal pathogen infection. |
| format | Article |
| id | doaj-art-676a0ff4703c45baab96f7e88ee5b759 |
| institution | Kabale University |
| issn | 1756-4646 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj-art-676a0ff4703c45baab96f7e88ee5b7592025-01-12T05:24:49ZengElsevierJournal of Functional Foods1756-46462025-01-01124106659Antimicrobial potential and mechanism of infant feces-derived Enterococcus faecalis in inhibiting Enterotoxigenic Escherichia coliXi Lu0Wei Sang1Lei Pan2School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021, China; Corresponding author.School of Food Science and Engineering, Shaanxi University of Science and Technology, Xi'an 710021, ChinaTangdu Hospital, Air Force Military Medical University, Xi'an 710032, ChinaEnterotoxigenic Escherichia coli (ETEC) is a leading cause of diarrhea in infants and travelers. However, antibiotic treatment is challenged by growing resistance. This study investigated the potential of Enterococcus faecalis, an early colonizer of the infant gut, as a probiotic to combat ETEC. From 125 healthy infant feces, 54 E. faecalis strains were isolated and evaluated for safety and function. E. faecalis LX25 and LX39 showed significant inhibition of ETEC growth, virulence gene (eltA and stA) expression (P < 0.05) and disrupted biofilm formation. Notably, LX39's cell-free supernatant (CFS) increased reactive oxygen species in ETEC, leading to cellular damage. HPLC revealed the three most concentrated organic acids, lactic (10.31 mg/mL), acetic (1.72 mg/mL) and citric acids (1.58 mg/mL) in LX39's CFS, which exceeding or approaching the MIC for ETEC. These findings indicate that E. faecalis LX39 could serve as effective probiotics candidates to inhibit ETEC, highlighting their potential in managing intestinal pathogen infection.http://www.sciencedirect.com/science/article/pii/S1756464625000015E. faecalisEnterotoxigenic Escherichia coliROSAntibacterial |
| spellingShingle | Xi Lu Wei Sang Lei Pan Antimicrobial potential and mechanism of infant feces-derived Enterococcus faecalis in inhibiting Enterotoxigenic Escherichia coli Journal of Functional Foods E. faecalis Enterotoxigenic Escherichia coli ROS Antibacterial |
| title | Antimicrobial potential and mechanism of infant feces-derived Enterococcus faecalis in inhibiting Enterotoxigenic Escherichia coli |
| title_full | Antimicrobial potential and mechanism of infant feces-derived Enterococcus faecalis in inhibiting Enterotoxigenic Escherichia coli |
| title_fullStr | Antimicrobial potential and mechanism of infant feces-derived Enterococcus faecalis in inhibiting Enterotoxigenic Escherichia coli |
| title_full_unstemmed | Antimicrobial potential and mechanism of infant feces-derived Enterococcus faecalis in inhibiting Enterotoxigenic Escherichia coli |
| title_short | Antimicrobial potential and mechanism of infant feces-derived Enterococcus faecalis in inhibiting Enterotoxigenic Escherichia coli |
| title_sort | antimicrobial potential and mechanism of infant feces derived enterococcus faecalis in inhibiting enterotoxigenic escherichia coli |
| topic | E. faecalis Enterotoxigenic Escherichia coli ROS Antibacterial |
| url | http://www.sciencedirect.com/science/article/pii/S1756464625000015 |
| work_keys_str_mv | AT xilu antimicrobialpotentialandmechanismofinfantfecesderivedenterococcusfaecalisininhibitingenterotoxigenicescherichiacoli AT weisang antimicrobialpotentialandmechanismofinfantfecesderivedenterococcusfaecalisininhibitingenterotoxigenicescherichiacoli AT leipan antimicrobialpotentialandmechanismofinfantfecesderivedenterococcusfaecalisininhibitingenterotoxigenicescherichiacoli |