Structural basis for substrate recognition mechanism of human SLC26A7

Abstract Solute carrier family 26 (SLC26) mainly mediates transmembrane transport of various anion ions, including chloride and other halide ions, bicarbonate, oxalate, and sulfate. Many severe hereditary human diseases are correlated with SLC26 protein mutations. Here we report cryo-EM structures o...

Full description

Saved in:
Bibliographic Details
Main Authors: Xiaorong Li, Xiaoxu Yang, Xiaoli Lu, Bingqian Lin, Yuanyuan Zhang, Bangdong Huang, Yutong Zhou, Jing Huang, Kun Wu, Qiang Zhou, Ximin Chi
Format: Article
Language:English
Published: Nature Portfolio 2025-08-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-62792-w
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Solute carrier family 26 (SLC26) mainly mediates transmembrane transport of various anion ions, including chloride and other halide ions, bicarbonate, oxalate, and sulfate. Many severe hereditary human diseases are correlated with SLC26 protein mutations. Here we report cryo-EM structures of human SLC26A7 in apo and iodide binding states. We identify non-canonical binding site for halide ions in SLC26A7. Molecular dynamics simulation and electrophysiological assay confirm the functional importance of key residues involved in iodide and chloride coordination. Together, our discovery marks a step towards an in-depth understanding of SLC26 family protein transport mechanisms.
ISSN:2041-1723