Changes in microbiome composition during ontogeny and dispersal of the coral boring sponge Thoosa mismalolli
Abstract Dispersal is an important life history trait that plays a key role in the demography and evolution of species. We employed a combined approach of DNA sequencing and transmission electron microscopy to examine the changes in the microbiome during the ontogeny and dispersal of the coral-excav...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-025-85622-x |
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| author | Eric Bautista-Guerrero José Luis Carballo Fabián Alejandro Rodríguez Zaragoza Bruno Goméz-Gil Alejandra García-Gasca Alma P. Rodríguez-Troncoso Adrián González-Castillo |
| author_facet | Eric Bautista-Guerrero José Luis Carballo Fabián Alejandro Rodríguez Zaragoza Bruno Goméz-Gil Alejandra García-Gasca Alma P. Rodríguez-Troncoso Adrián González-Castillo |
| author_sort | Eric Bautista-Guerrero |
| collection | DOAJ |
| description | Abstract Dispersal is an important life history trait that plays a key role in the demography and evolution of species. We employed a combined approach of DNA sequencing and transmission electron microscopy to examine the changes in the microbiome during the ontogeny and dispersal of the coral-excavating sponge Thoosa mismalolli. The results show that sponge can acquired their associated bacteria via both vertical (VT) and horizontal transmission (HT). Adult sponges, brooding larvae, and early free-swimming sponge larvae harbor a similar high-diversity microbial assemblage, dominated by Proteobacteria and Chloroflexi, which change throughout the larval dispersal phase. Larvae collected offshore showed a reorganization of their microbiome with a significant reduction of the dominance of inherited bacteria (Proteobacteria and Chloroflexi), and an enrichment of environmentally derived bacteria taxa (Bacteroidetes, Tenericutes, and Firmicutes). TEM confirmed a substantial change in cell structure and microbial composition, attributed to symbionts’ massive phagocytosis. This research provides information on microbiome dynamics through the sponge ontogeny and sheds on their possible role in the dispersal capacity of their larvae. |
| format | Article |
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| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-6748a7daeb0949adbab9c73cf1f4eea02025-01-19T12:17:26ZengNature PortfolioScientific Reports2045-23222025-01-0115111810.1038/s41598-025-85622-xChanges in microbiome composition during ontogeny and dispersal of the coral boring sponge Thoosa mismalolliEric Bautista-Guerrero0José Luis Carballo1Fabián Alejandro Rodríguez Zaragoza2Bruno Goméz-Gil3Alejandra García-Gasca4Alma P. Rodríguez-Troncoso5Adrián González-Castillo6Laboratorio de Ecología Marina, Centro de Investigaciones Costeras, Centro Universitario de la Costa, Universidad de GuadalajaraLaboratorio de Biología Marina, Departamento de Zoología, Facultad de Biología, Universidad de SevillaLaboratorio de Ecología Molecular, Microbiología y Taxonomía (LEMITAX), Departamento de Ecología Aplicada, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de GuadalajaraCentro de Investigación en Alimentación y Desarrollo, Mazatlán Unit for Aquaculture and Environmental ManagementLaboratorio de Biología Molecular, Centro de Investigación en Alimentación y Desarrollo, A.C.Laboratorio de Ecología Marina, Centro de Investigaciones Costeras, Centro Universitario de la Costa, Universidad de GuadalajaraLaboratorio de Bioinformática Microbiana, Programa Académico de Ingeniería en Biotecnología, Universidad Politécnica de SinaloaAbstract Dispersal is an important life history trait that plays a key role in the demography and evolution of species. We employed a combined approach of DNA sequencing and transmission electron microscopy to examine the changes in the microbiome during the ontogeny and dispersal of the coral-excavating sponge Thoosa mismalolli. The results show that sponge can acquired their associated bacteria via both vertical (VT) and horizontal transmission (HT). Adult sponges, brooding larvae, and early free-swimming sponge larvae harbor a similar high-diversity microbial assemblage, dominated by Proteobacteria and Chloroflexi, which change throughout the larval dispersal phase. Larvae collected offshore showed a reorganization of their microbiome with a significant reduction of the dominance of inherited bacteria (Proteobacteria and Chloroflexi), and an enrichment of environmentally derived bacteria taxa (Bacteroidetes, Tenericutes, and Firmicutes). TEM confirmed a substantial change in cell structure and microbial composition, attributed to symbionts’ massive phagocytosis. This research provides information on microbiome dynamics through the sponge ontogeny and sheds on their possible role in the dispersal capacity of their larvae.https://doi.org/10.1038/s41598-025-85622-xDispersalLarvaMicrobiomeVertical transmissionPhagocytosis |
| spellingShingle | Eric Bautista-Guerrero José Luis Carballo Fabián Alejandro Rodríguez Zaragoza Bruno Goméz-Gil Alejandra García-Gasca Alma P. Rodríguez-Troncoso Adrián González-Castillo Changes in microbiome composition during ontogeny and dispersal of the coral boring sponge Thoosa mismalolli Scientific Reports Dispersal Larva Microbiome Vertical transmission Phagocytosis |
| title | Changes in microbiome composition during ontogeny and dispersal of the coral boring sponge Thoosa mismalolli |
| title_full | Changes in microbiome composition during ontogeny and dispersal of the coral boring sponge Thoosa mismalolli |
| title_fullStr | Changes in microbiome composition during ontogeny and dispersal of the coral boring sponge Thoosa mismalolli |
| title_full_unstemmed | Changes in microbiome composition during ontogeny and dispersal of the coral boring sponge Thoosa mismalolli |
| title_short | Changes in microbiome composition during ontogeny and dispersal of the coral boring sponge Thoosa mismalolli |
| title_sort | changes in microbiome composition during ontogeny and dispersal of the coral boring sponge thoosa mismalolli |
| topic | Dispersal Larva Microbiome Vertical transmission Phagocytosis |
| url | https://doi.org/10.1038/s41598-025-85622-x |
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