Neuroprotective effects of quercetin on hippocampal CA1 neurons following middle cerebral artery ischemia‒reperfusion in male rats: a behavioral, biochemical, and histological study
Abstract Introduction Cerebral ischemic strokes cause brain damage, primarily through inflammatory factors. One of the regions most affected by middle cerebral artery occlusion (MCAO) is the hippocampus, specifically the CA1 area, which is highly susceptible to ischemia. Previous studies have demons...
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2025-01-01
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| Online Access: | https://doi.org/10.1186/s12883-024-04017-z |
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| author | Mehran Mahyar Erfan Ghadirzadeh Pedram Nezhadnaderi Zahrasadat Moayedi Parniyan Maboud Arvin Ebrahimi Ali Siahposht-Khachaki Narges Karimi |
| author_facet | Mehran Mahyar Erfan Ghadirzadeh Pedram Nezhadnaderi Zahrasadat Moayedi Parniyan Maboud Arvin Ebrahimi Ali Siahposht-Khachaki Narges Karimi |
| author_sort | Mehran Mahyar |
| collection | DOAJ |
| description | Abstract Introduction Cerebral ischemic strokes cause brain damage, primarily through inflammatory factors. One of the regions most affected by middle cerebral artery occlusion (MCAO) is the hippocampus, specifically the CA1 area, which is highly susceptible to ischemia. Previous studies have demonstrated the anti-inflammatory properties of quercetin. Therefore, this study aimed to investigate the neuroprotective effects of quercetin on hippocampal CA1 neurons following MCAO. Materials and methods Fifty-six male Albino Wistar rats were divided into seven groups (intact, sham, stroke, vehicle, and three quercetin-treated groups receiving 5, 10, and 20 mg/kg, respectively), each containing 8 rats. Various assessments, including brain water content, the rotarod test, the Bederson neurological score, the Morris water maze (MWM) test, the shuttle box test, histopathological evaluations, and measurements of interleukin-10 (IL-10) and interleukin-1β (IL-1β) levels, were conducted across the groups. Results Compared with control rats, 5 and 10 mg/kg quercetin-treated rats presented significant improvements in brain water content, neurological function, and motor function and improved performance in the MWM and shuttle box tests. Histopathological analyses revealed better preservation of CA1 neurons in these groups. Additionally, IL-10 levels significantly increased, whereas IL-1β levels significantly decreased. However, the group receiving 20 mg/kg quercetin showed no statistically significant changes in the parameters assessed (P > 0.05). Conclusion Quercetin may help prevent or ameliorate brain injuries caused by acute stroke, suggesting its neuroprotective effects. The reduction in IL-1β and increase in IL-10 may play key roles in quercetin’s protective mechanism. |
| format | Article |
| id | doaj-art-67378fdaf0ab472e8a1ef58f01ee79ed |
| institution | Kabale University |
| issn | 1471-2377 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Neurology |
| spelling | doaj-art-67378fdaf0ab472e8a1ef58f01ee79ed2025-01-12T12:28:45ZengBMCBMC Neurology1471-23772025-01-0125111410.1186/s12883-024-04017-zNeuroprotective effects of quercetin on hippocampal CA1 neurons following middle cerebral artery ischemia‒reperfusion in male rats: a behavioral, biochemical, and histological studyMehran Mahyar0Erfan Ghadirzadeh1Pedram Nezhadnaderi2Zahrasadat Moayedi3Parniyan Maboud4Arvin Ebrahimi5Ali Siahposht-Khachaki6Narges Karimi7Department of Neurology, Mazandaran University of Medical SciencesGastrointestitional Cancer Research Center, Non-Communicable Disease Institute, Mazandaran University of Medical SciencesStudent Research Committee, Shiraz University of Medical SciencesDepartment of Internal Medicine, School of Medicine, Shiraz University of Medical SciencesDepartment of Pharmacology Sciences, School of Pharmacy, Mazandaran University of Medical SciencesDepartment of Pharmacology Sciences, School of Pharmacy, Mazandaran University of Medical SciencesImmunogenetics Research Center, Department of Physiology, Mazandaran University of Medical SciencesDepartment of Neurology, School of Medicine, Immunogenetic Research Center, Mazandaran University of Medical SciencesAbstract Introduction Cerebral ischemic strokes cause brain damage, primarily through inflammatory factors. One of the regions most affected by middle cerebral artery occlusion (MCAO) is the hippocampus, specifically the CA1 area, which is highly susceptible to ischemia. Previous studies have demonstrated the anti-inflammatory properties of quercetin. Therefore, this study aimed to investigate the neuroprotective effects of quercetin on hippocampal CA1 neurons following MCAO. Materials and methods Fifty-six male Albino Wistar rats were divided into seven groups (intact, sham, stroke, vehicle, and three quercetin-treated groups receiving 5, 10, and 20 mg/kg, respectively), each containing 8 rats. Various assessments, including brain water content, the rotarod test, the Bederson neurological score, the Morris water maze (MWM) test, the shuttle box test, histopathological evaluations, and measurements of interleukin-10 (IL-10) and interleukin-1β (IL-1β) levels, were conducted across the groups. Results Compared with control rats, 5 and 10 mg/kg quercetin-treated rats presented significant improvements in brain water content, neurological function, and motor function and improved performance in the MWM and shuttle box tests. Histopathological analyses revealed better preservation of CA1 neurons in these groups. Additionally, IL-10 levels significantly increased, whereas IL-1β levels significantly decreased. However, the group receiving 20 mg/kg quercetin showed no statistically significant changes in the parameters assessed (P > 0.05). Conclusion Quercetin may help prevent or ameliorate brain injuries caused by acute stroke, suggesting its neuroprotective effects. The reduction in IL-1β and increase in IL-10 may play key roles in quercetin’s protective mechanism.https://doi.org/10.1186/s12883-024-04017-zQuercetinCerebral ischemiaNeuroprotectionFocal cerebral ischemiaMiddle cerebral artery occlusion |
| spellingShingle | Mehran Mahyar Erfan Ghadirzadeh Pedram Nezhadnaderi Zahrasadat Moayedi Parniyan Maboud Arvin Ebrahimi Ali Siahposht-Khachaki Narges Karimi Neuroprotective effects of quercetin on hippocampal CA1 neurons following middle cerebral artery ischemia‒reperfusion in male rats: a behavioral, biochemical, and histological study BMC Neurology Quercetin Cerebral ischemia Neuroprotection Focal cerebral ischemia Middle cerebral artery occlusion |
| title | Neuroprotective effects of quercetin on hippocampal CA1 neurons following middle cerebral artery ischemia‒reperfusion in male rats: a behavioral, biochemical, and histological study |
| title_full | Neuroprotective effects of quercetin on hippocampal CA1 neurons following middle cerebral artery ischemia‒reperfusion in male rats: a behavioral, biochemical, and histological study |
| title_fullStr | Neuroprotective effects of quercetin on hippocampal CA1 neurons following middle cerebral artery ischemia‒reperfusion in male rats: a behavioral, biochemical, and histological study |
| title_full_unstemmed | Neuroprotective effects of quercetin on hippocampal CA1 neurons following middle cerebral artery ischemia‒reperfusion in male rats: a behavioral, biochemical, and histological study |
| title_short | Neuroprotective effects of quercetin on hippocampal CA1 neurons following middle cerebral artery ischemia‒reperfusion in male rats: a behavioral, biochemical, and histological study |
| title_sort | neuroprotective effects of quercetin on hippocampal ca1 neurons following middle cerebral artery ischemia reperfusion in male rats a behavioral biochemical and histological study |
| topic | Quercetin Cerebral ischemia Neuroprotection Focal cerebral ischemia Middle cerebral artery occlusion |
| url | https://doi.org/10.1186/s12883-024-04017-z |
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